AB3980

Anti-alpha 1 Fetoprotein antibody [AFP-01]

Name: Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980) | Abcam
Brand: Abcam Limited
SKU: AB3980
Price: 435 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(48 Publications)

Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980) is a mouse monoclonal antibody detecting alpha 1 Fetoprotein in ICC/IF. Suitable for Human.

- Over 40 publications
- Trusted since 2003

View Alternative Names

HPAFP, AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (AB3980)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (AB3980)

ICC/IF image of ab3980 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3980, 5μg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor^® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AFP-01

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human AFP.

P02771

Specificity

The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980) is a mouse monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-alpha 1 Fetoprotein [AFP-01] (ab3980) was first used in a scientific publication in 2003 and has been cited over 40 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Purified by precipitation and chromatography

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.

Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

See full target information AFP

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Publications (48)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 11:e16358 PubMed38025691

2023

Comparative study of the efficacy of intra-arterial and intravenous transplantation of human induced pluripotent stem cells-derived neural progenitor cells in experimental stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Elvira Cherkashova,Daria Namestnikova,Georgiy Leonov,Ilya Gubskiy,Kirill Sukhinich,Pavel Melnikov,Vladimir Chekhonin,Konstantin Yarygin,Dmitry Goldshtein,Diana Salikhova

iScience 26:107675 PubMed37680467

2023

Integrative analysis of single-cell RNA-seq and ATAC-seq reveals heterogeneity of induced pluripotent stem cell-derived hepatic organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Jong-Hwan Kim,Seon Ju Mun,Jeong-Hwan Kim,Myung Jin Son,Seon-Young Kim

Translational oncology 36:101737 PubMed37478671

2023

Characteristics of alpha-fetoprotein-positive gastric cancer revealed by analysis of cancer databases and transcriptome sequencing data.

Applications

Unspecified application

Species

Unspecified reactive species

Yansen Li,Yilin Lin,Long Zhao,Changjiang Yang,Bo Wang,Zhidong Gao,Yingjiang Ye,Shan Wang,Zhanlong Shen

eLife 12: PubMed36961502

2023

Multi-targeted therapy resistance via drug-induced secretome fucosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Borris D Aldonza,Junghwa Cha,Insung Yong,Jayoung Ku,Pavel Sinitcyn,Dabin Lee,Ryeong-Eun Cho,Roben D Delos Reyes,Dongwook Kim,Soyeon Kim,Minjeong Kang,Yongsuk Ku,Geonho Park,Hye-Jin Sung,Han Suk Ryu,Sukki Cho,Tae Min Kim,Pilnam Kim,Je-Yoel Cho,Yoosik Kim

Nature communications 14:237 PubMed36646721

2023

Integrative analysis of multiple genomic data from intrahepatic cholangiocarcinoma organoids enables tumor subtyping.

Applications

Unspecified application

Species

Unspecified reactive species

Hee Seung Lee,Dai Hoon Han,Kyungjoo Cho,Soo Been Park,Chanyang Kim,Galam Leem,Dawoon E Jung,Soon Sung Kwon,Chul Hoon Kim,Jung Hyun Jo,Hye Won Lee,Si Young Song,Jun Yong Park

Cancers 14: PubMed35681712

2022

Anti-Cancer Effects of YAP Inhibitor (CA3) in Combination with Sorafenib against Hepatocellular Carcinoma (HCC) in Patient-Derived Multicellular Tumor Spheroid Models (MCTS).

Applications

Unspecified application

Species

Unspecified reactive species

Sojung Han,Ji Yeon Lim,Kyungjoo Cho,Hye Won Lee,Jun Yong Park,Simon Weonsang Ro,Kyung Sik Kim,Haeng Ran Seo,Do Young Kim

Biology 11: PubMed35453693

2022

Elimination of Reprogramming Transgenes Facilitates the Differentiation of Induced Pluripotent Stem Cells into Hepatocyte-like Cells and Hepatic Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Jaemin Jeong,Tae Hun Kim,Myounghoi Kim,Yun Kyung Jung,Kyeong Sik Kim,Sehwan Shim,Hyosun Jang,Won Il Jang,Seung Bum Lee,Dongho Choi

Disease models & mechanisms 15: PubMed35191981

2022

Development and characterization of cell models harbouring mtDNA deletions for in vitro study of Pearson syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Hernández-Ainsa,Ester López-Gallardo,María Concepción García-Jiménez,Francisco José Climent-Alcalá,Carmen Rodríguez-Vigil,Marta García Fernández de Villalta,Rafael Artuch,Julio Montoya,Eduardo Ruiz-Pesini,Sonia Emperador

Oncology letters 23:84 PubMed35126726

2022

Alpha-fetoprotein can promote gastric cancer progression via upregulation of metastasis-associated colon cancer 1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Mao,Jun Wang,Fen Luo

Stem cell research 59:102632 PubMed34973561

2022

Generation of an induced pluripotent stem cell line from a compound heterozygous patient in TK2 gene.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Hernández-Ainsa,Andrés Nascimento,Cristina Jou,Rafael Artuch,Julio Montoya,Eduardo Ruiz-Pesini,Sonia Emperador

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Fetoprotein antibody [AFP-01]

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (AB3980)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (48)

Product promise