Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980) is a mouse monoclonal antibody that is used to detect alpha 1 Fetoprotein in ICC/IF. Suitable for Human samples.
- Over 40 publications
- Trusted since 2003
Images
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (AB3980), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-afp-01-ab3980--immunocytochemistry-immunofluorescence-img105819.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (AB3980)")
Publications
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- PeerJ 11:e163582023Comparative study of the efficacy of intra-arterial and intravenous transplantation of human induced pluripotent stem cells-derived neural progenitor cells in experimental stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElvira Cherkashova,Daria Namestnikova,Georgiy Leonov,Ilya Gubskiy,Kirill Sukhinich,Pavel Melnikov,Vladimir Chekhonin,Konstantin Yarygin,Dmitry Goldshtein,Diana SalikhovaPubMed 38025691
- iScience 26:1076752023Integrative analysis of single-cell RNA-seq and ATAC-seq reveals heterogeneity of induced pluripotent stem cell-derived hepatic organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJong-Hwan Kim,Seon Ju Mun,Jeong-Hwan Kim,Myung Jin Son,Seon-Young KimPubMed 37680467
- Nature communications 14:2372023Integrative analysis of multiple genomic data from intrahepatic cholangiocarcinoma organoids enables tumor subtyping.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHee Seung Lee,Dai Hoon Han,Kyungjoo Cho,Soo Been Park,Chanyang Kim,Galam Leem,Dawoon E Jung,Soon Sung Kwon,Chul Hoon Kim,Jung Hyun Jo,Hye Won Lee,Si Young Song,Jun Yong ParkPubMed 36646721
- Cancers 14:2022Anti-Cancer Effects of YAP Inhibitor (CA3) in Combination with Sorafenib against Hepatocellular Carcinoma (HCC) in Patient-Derived Multicellular Tumor Spheroid Models (MCTS).Applications:Unspecified applicationReactive species:Unspecified reactive speciesSojung Han,Ji Yeon Lim,Kyungjoo Cho,Hye Won Lee,Jun Yong Park,Simon Weonsang Ro,Kyung Sik Kim,Haeng Ran Seo,Do Young KimPubMed 35681712
- Biology 11:2022Elimination of Reprogramming Transgenes Facilitates the Differentiation of Induced Pluripotent Stem Cells into Hepatocyte-like Cells and Hepatic Organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaemin Jeong,Tae Hun Kim,Myounghoi Kim,Yun Kyung Jung,Kyeong Sik Kim,Sehwan Shim,Hyosun Jang,Won Il Jang,Seung Bum Lee,Dongho ChoiPubMed 35453693
- Disease models & mechanisms 15:2022Development and characterization of cell models harbouring mtDNA deletions for in vitro study of Pearson syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmen Hernández-Ainsa,Ester López-Gallardo,María Concepción García-Jiménez,Francisco José Climent-Alcalá,Carmen Rodríguez-Vigil,Marta García Fernández de Villalta,Rafael Artuch,Julio Montoya,Eduardo Ruiz-Pesini,Sonia EmperadorPubMed 35191981
- Oncology letters 23:842022Alpha-fetoprotein can promote gastric cancer progression via upregulation of metastasis-associated colon cancer 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Mao,Jun Wang,Fen LuoPubMed 35126726
- Scientific reports 11:189162021Neuronal mitochondrial dysfunction in sporadic amyotrophic lateral sclerosis is developmentally regulated.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTanisha Singh,Yuanyuan Jiao,Lisa M Ferrando,Svitlana Yablonska,Fang Li,Emily C Horoszko,David Lacomis,Robert M Friedlander,Diane L CarlislePubMed 34556702
- iScience 24:1025522021Dimethyl fumarate reduces hepatocyte senescence following paracetamol exposure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJose Meseguer-Ripolles,Baltasar Lucendo-Villarin,Carl Tucker,Sofia Ferreira-Gonzalez,Natalie Homer,Yu Wang,Philip J Starkey Lewis,Enrique M Toledo,Esther Mellado-Gomez,Joanna Simpson,Oliver Flint,Himjyot Jaiswal,Nicola L Beer,Allan E Karlsen,Stuart J Forbes,James W Dear,Jeremy Hughes,David C HayPubMed 34151225
- International journal of molecular sciences 22:2021Therapeutic Effects of hiPSC-Derived Glial and Neuronal Progenitor Cells-Conditioned Medium in Experimental Ischemic Stroke in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiana Salikhova,Tatiana Bukharova,Elvira Cherkashova,Daria Namestnikova,Georgy Leonov,Maria Nikitina,Ilya Gubskiy,Gevorg Akopyan,Andrey Elchaninov,Konstantin Midiber,Natalia Bulatenco,Victoria Mokrousova,Andrey Makarov,Konstantin Yarygin,Vladimir Chekhonin,Liudmila Mikhaleva,Timur Fatkhudinov,Dmitry GoldshteinPubMed 33946667
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human AFP. Database link P02771
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Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Associated Products
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Target data
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Alternative names
HPAFP, AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin
Recommended products
Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980) is a mouse monoclonal antibody that is used to detect alpha 1 Fetoprotein in ICC/IF. Suitable for Human samples.
- Over 40 publications
- Trusted since 2003
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human AFP. Database link P02771
- Clone number
- AFP-01
- Purification technique
- Proprietary technique
- Specificity
The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
- Light chain type
- unknown
- Concentration
- Purification notes
Purified by precipitation and chromatography
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980) is a mouse monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Trusted by the scientific community
Anti-alpha 1 Fetoprotein [AFP-01] (ab3980) was first used in a scientific publication in 2003 and has been cited over 40 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.
- Biological function summary
Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.
- Pathways
Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.
- Associated diseases and disorders
High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-afp-01-ab3980--immunocytochemistry-immunofluorescence-img105819.jpg "Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)")
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
ICC/IF image of ab3980 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3980, 5μg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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