AB133617

Anti-alpha 1 Fetoprotein antibody [EPAFP61]

RabMAb
Recombinant
20ul selling size
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(11 Publications)

Rabbit Recombinant Monoclonal alpha 1 Fetoprotein antibody. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications.

View Alternative Names

HPAFP, AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling alpha 1 Fetoprotein with Purified ab133617 at 1/250 dilution (0.52 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue sections labeling alpha 1 Fetoprotein with Purified ab133617 at 1/100 dilution (1.3 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

Intracellular Flow Cytometry analysis of HepG2 Human hepatocellular carcinoma epithelial cell cells labeling alpha 1 Fetoprotein with purified ab133617 at 1/100 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

Lab

Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

Purified ab133617 at 1/20 dilution (0.5μg) immunoprecipitating alpha 1 Fetoprotein in HepG2 whole cell lysate.
Lane 1 (input) : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab133617 + HepG2 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab133617 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 70 kDa
Lower bands could be caspase-dependent cleavage fragments. (PMID : 31811908)

All lanes:

Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)

Predicted band size: 68 kDa

false

Unknown

Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

All lanes:

Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617) at 1/10000 dilution

All lanes:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 68 kDa

false

Carrier free

Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-alpha 1 Fetoprotein antibody [EPAFP61]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-alpha 1 Fetoprotein antibody [EPAFP61]
Carrier free

Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPAFP61

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IP, ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98°C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.

Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

See full target information AFP

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1232 PubMed40817274

2025

STRaM: A genetic framework for improved cell product provenance for research and clinical translations.

Applications

Unspecified application

Species

Unspecified reactive species

Binglin Li,Chi Le,Wen Lei,Yingqi Zhou,Ying Zhang,Yanwen Wang,Yanyan Wang,Xian Li,Weiyan Zheng,Jie Sun,Yuanbiao Tu,Wangren Yang,Kuncheng Zhou,Stephene S Meena,Yufei Li,Keying Zhu,Shuqin Zhou,Liyan Liu,Hao Chen,Qing Peng,Wenbin Qian,Ray P S Han,Wei Guo

Nature communications 16:6451 PubMed40651942

2025

Androgen receptor promotes arachidonic acid metabolism and angiogenic microenvironment in AFP-negative hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhilong Lin,Xiaofei Liu,Houwei Wang,Shumin Li,Ziqiang Miao,Jing Yang,Yuting Zhang,Kai Lei,Yifan Wu,Youmei Kang,Ruoyin Zheng,Zonglin Xie,Yixi Wen,Xiaoxue Ren,Chunxiao Liu,Alfred Sze-Lok Cheng,Yubin Xie,Shuling Chen,Ming Kuang,Sui Peng,Zhenwei Peng,Zihao Dai

Cells 11: PubMed35455938

2022

Decellularized Organ-Derived Scaffold Is a Promising Carrier for Human Induced Pluripotent Stem Cells-Derived Hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hideaki Kojima,Hiroshi Yagi,Hiroko Kushige,Yukiko Toda,Kazuo Takayama,Shinako Masuda,Toshinori Morisaku,Tomonori Tsuchida,Kohei Kuroda,Kazuya Hirukawa,Jumpei Inui,Kotaro Nishi,Yutaka Nakano,Masayuki Tanaka,Shutaro Hori,Yasushi Hasegawa,Yuta Abe,Minoru Kitago,Shungo Adachi,Masatoshi Tomi,Katsuhisa Matsuura,Hiroyuki Mizuguchi,Yuko Kitagawa

International journal of molecular sciences 22: PubMed34576243

2021

Profiling of Human Neural Crest Chemoattractant Activity as a Replacement of Fetal Bovine Serum for In Vitro Chemotaxis Assays.

Applications

Unspecified application

Species

Unspecified reactive species

Xenia Dolde,Christiaan Karreman,Marianne Wiechers,Stefan Schildknecht,Marcel Leist

Cells 10: PubMed33918222

2021

Additional Inhibition of Wnt/β-Catenin Signaling by Metformin in DAA Treatments as a Novel Therapeutic Strategy for HCV-Infected Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lin,Venu Reddy,Hanadi Osman,Adriana Lopez,Ali Riza Koksal,Sadeq Mutlab Rhadhi,Srikanta Dash,Yucel Aydin

Scientific reports 10:4272 PubMed32144293

2020

Generation and characterization of human induced pluripotent stem cells (iPSCs) from hand osteoarthritis patient-derived fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

R Castro-Viñuelas,C Sanjurjo-Rodríguez,M Piñeiro-Ramil,T Hermida-Gómez,S Rodríguez-Fernández,N Oreiro,J de Toro,I Fuentes,F J Blanco,S Díaz-Prado

Pediatric research 87:3-6 PubMed31412353

2019

Proliferating infantile hemangioma promotes α-fetoprotein production by HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bede van Schaijik,Jennifer de Jongh,Reginald W Marsh,Matthew Munro,Tinte Itinteang,Swee T Tan

Cellular reprogramming 20:356-364 PubMed30388030

2018

Generation of Induced Pluripotent Stem Cells from Human Epidermal Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hua Yang,Ru-Zhi Zhang,Sai Cheng,Bin Xu,Ting Tian,Hai-Xia Shi,Li Xiao,Ren-He Chen

Scientific reports 8:1799 PubMed29379065

2018

Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver M Russell,Isabelle Fruh,Pavandeep K Rai,David Marcellin,Thierry Doll,Amy Reeve,Mitchel Germain,Julie Bastien,Karolina A Rygiel,Raffaele Cerino,Andreas W Sailer,Majlinda Lako,Robert W Taylor,Matthias Mueller,Robert N Lightowlers,Doug M Turnbull,Stephen B Helliwell

European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes 58:27-39 PubMed27598993

2016

Mesenchymal Stem Cells Contribute to Hepatic Maturation of Human Induced Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chisato Takagi,Hiroshi Yagi,Makiko Hieda,Kazuki Tajima,Taizo Hibi,Yuta Abe,Minoru Kitago,Masahiro Shinoda,Osamu Itano,Yuko Kitagawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Fetoprotein antibody [EPAFP61]

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (AB133617)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise