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Rabbit Recombinant Monoclonal alpha 1 Fetoprotein antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse samples. Cited in 1 publication.

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Images

Western blot - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (AB199054), expandable thumbnail
  • Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (AB199054), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (AB199054), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Mouse
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Binds estrogens, fatty acids and metals.

Alternative names

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Afp

Recommended products

Rabbit Recombinant Monoclonal alpha 1 Fetoprotein antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19240-133
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.

Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

Associated diseases and disorders

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054), expandable thumbnail

    Western blot - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)

    Exposure time : Lanes 1 and 2: 3 seconds; Lane 3: 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed molecular weight is consistent with the literature (PMID: 18525461).

    All lanes: Western blot - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054) at 1/1000 dilution

    Lane 1: Mouse placenta lysate at 20 µg

    Lane 2: Mouse fetal liver lysate at 20 µg

    Lane 3: Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa

    Observed band size: 70 kDa

  • Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054), expandable thumbnail

    Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)

    alpha 1 Fetoprotein was immunoprecipitated from 0.35 mg of mouse placenta lysate with ab199054 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199054 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse placenta lysate 10 μg (Input).

    Lane 2: ab199054 IP in mouse placenta lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199054 in mouse placenta lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)

    Predicted band size: 68 kDa

    Observed band size: 70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling alpha 1 Fetoprotein with ab199054 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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