AB213328

Anti-alpha 1 Fetoprotein antibody [EPR20667]

Name: Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328) | Abcam
Brand: Abcam Limited
SKU: AB213328
Price: 205 USD
Availability: InStock
Rating: 5 (1 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(11 Publications)

Rabbit Recombinant Monoclonal alpha 1 Fetoprotein antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 11 publications.

View Alternative Names

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Afp

3 Images

Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPR20667] (AB213328)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPR20667] (AB213328)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling alpha 1 Fetoprotein with ab213328 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR20667] (AB213328)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR20667] (AB213328)

Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling alpha 1 Fetoprotein with ab213328 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Supplier Data

Western blot - Anti-alpha 1 Fetoprotein antibody [EPR20667] (AB213328)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 10 seconds; Lane 2/4 : 1 second; Lane 3 : 3 seconds.

The observed molecular weight is consistent with the literature (PMID : 18525461).

Lanes 1 - 2:

Western blot - Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328) at 1/1000 dilution

Lanes 3 - 4:

Western blot - Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328) at 1/5000 dilution

Lane 1:

Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate at 20 µg

Lane 2:

Rat placenta lysate at 20 µg

Lane 3:

Mouse fetal liver lysate at 20 µg

Lane 4:

Mouse placenta lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 70 kDa

true

Carrier free

Anti-alpha 1 Fetoprotein antibody [EPR20667] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20667

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.

Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds estrogens, fatty acids and metals.

See full target information Afp

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40564980

2025

Increased Plasma Levels of ACE and Ang II in Prediabetes May Contribute to Adipose Tissue Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Bongeka Cassandra Mkhize,Palesa Mosili,Phikelelani Sethu Ngubane,Ntethelelo Hopewell Sibiya,Andile Khathi

International journal of molecular sciences 24: PubMed36982757

2023

HPF Modulates the Differentiation of BMSCs into HLCs and Promotes the Recovery of Acute Liver Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Yang,Qiu-Lin Luo,Nan Wang,Yan-Ting Hu,Wen-Xin Zheng,Hong Li,Maimaitituxun Maierziya,Jian Gu,Qin Wang

International journal of molecular sciences 23: PubMed36430764

2022

Leucine and Arginine Availability Modulate Mouse Embryonic Stem Cell Proliferation and Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Bibiana Correia,Maria Inês Sousa,Ana Filipa Branco,Ana Sofia Rodrigues,João Ramalho-Santos

World journal of gastrointestinal oncology 14:858-871 PubMed35582105

2022

Bi-specific T1 positive-contrast-enhanced magnetic resonance imaging molecular probe for hepatocellular carcinoma in an orthotopic mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Hong Ma,Kun Chen,Shuang Wang,Si-Yun Liu,Deng-Feng Li,Yong-Tao Mi,Zhi-Yuan Wu,Chun-Feng Qu,Xin-Ming Zhao

Cell death discovery 8:77 PubMed35194023

2022

Long noncoding RNA SNHG1 silencing accelerates hepatocyte-like cell differentiation of bone marrow-derived mesenchymal stem cells to alleviate cirrhosis via the microRNA-15a/SMURF1/UVRAG axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Sun,Xuedong Sun,Sean Hu,Maoqiang Wang,Na Ma,Junhui Chen,Feng Duan

Cell death discovery 8:51 PubMed35136027

2022

ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Liu,Chengqian Lv,Qianqian Huang,Lei Zhao,Xiaoli Sun,Dandan Ning,Jingyang Liu,Yanan Jiang,Shizhu Jin

Archives of toxicology 95:2413-2430 PubMed34050779

2021

Proteomic profiling of murine biliary-derived hepatic organoids and their capacity for drug disposition, bioactivation and detoxification.

Applications

Unspecified application

Species

Unspecified reactive species

Lawrence Howell,Rosalind E Jenkins,Stephen Lynch,Carrie Duckworth,B Kevin Park,Christopher Goldring

Nature communications 11:4283 PubMed32883967

2020

In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyoung Lee,Akito Sutani,Rin Kaneko,Jun Takeuchi,Tetsuo Sasano,Takashi Kohda,Kensuke Ihara,Kentaro Takahashi,Masahiro Yamazoe,Tomohiro Morio,Tetsushi Furukawa,Fumitoshi Ishino

Journal of cellular biochemistry 121:3941-3951 PubMed31696964

2019

Ring1 promotes the transformation of hepatic progenitor cells into cancer stem cells through the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhu,Jiangwei Li,Jun Li,Jin Sun,Ying Guo,Hongwei Tian,Liang Li,Chen Zhang,Mengjiao Shi,Guangyao Kong,Zongfang Li

EBioMedicine 46:119-132 PubMed31375423

2019

Hepatectomy promotes recurrence of liver cancer by enhancing IL-11-STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyao Wang,Xiaohu Zheng,Binqing Fu,Zhigang Nian,Yeben Qian,Rui Sun,Zhigang Tian,Haiming Wei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Fetoprotein antibody [EPR20667]

Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPR20667] (AB213328)

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR20667] (AB213328)

Western blot - Anti-alpha 1 Fetoprotein antibody [EPR20667] (AB213328)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise