AB255813

Anti-alpha 1 Fetoprotein antibody [EPR22560-268]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal alpha 1 Fetoprotein antibody. Suitable for IP, WB and reacts with Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Afp

3 Images

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Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (AB255813)

alpha 1 Fetoprotein was immunoprecipitated from 0.35 mg rat placenta lysate with ab255813 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255813 at 1/1000 dilution (0.56 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at a 1/5000 dilution.

Lane 1 : Rat placenta lysate 10μg
Lane 2 : ab255813 IP in rat placenta lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab255813 in rat placenta lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 3 secs.

All lanes:

Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813)

Predicted band size: 68 kDa

Observed band size: 70 kDa

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Western blot - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (AB255813)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 25355916).

Negative control : Rat brain (PMID : 2410773, 6175646 ).

Exposure times : Lane 1 : 1 sec; Lanes 2-3 : 3 mins.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813) at 1/1000 dilution

Lane 1:

Rat placenta tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 70 kDa

false

Unknown

Western blot - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (AB255813)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 25355916).

Exposure times : Lane 1 : 3 mins; Lane 2 : 5.5 secs.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813) at 1/1000 dilution

Lane 1:

Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Mouse placenta tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 70 kDa

false

Carrier free

Anti-alpha 1 Fetoprotein antibody [EPR22560-268] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22560-268

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/30 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/30 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.

Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds estrogens, fatty acids and metals.

See full target information Afp

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1380 PubMed39443661

2024

Deficiencies in corin and atrial natriuretic peptide-mediated signaling impair endochondral ossification in bone development.

Applications

Unspecified application

Species

Unspecified reactive species

Zibin Zhou,Xiaoyu Mao,Chun Jiang,Wenguo Li,Tiantian Zhou,Meng Liu,Shijin Sun,Mengting Wang,Ningzheng Dong,Qingyu Wu,Haibin Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Fetoprotein antibody [EPR22560-268]

Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (AB255813)

Western blot - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (AB255813)

Western blot - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (AB255813)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise