Anti-alpha 1 Fetoprotein antibody [EPR26548-19] is a Rabbit Monoclonal antibody that is used in alpha 1 Fetoprotein IHC-P, Western Blot. Suitable for Mouse, Rat samples.
Alpha-fetoprotein (AFP) is a key biomarker for diagnosing hepatocellular carcinoma (HCC), with elevated levels indicating liver cancer. It contributes to an immune-tolerant tumor microenvironment, helping cancer cells evade immune detection. AFP is being explored as a therapeutic target for liver cancer using CAR-T cells but the major focus is using AFP as a diagnostic or prognostic marker for liver, colorectal and gastric cancers.
Images
![Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (AB290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--western-blot-img167073.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (AB290637)")
![Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (AB290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--western-blot-img170128.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (AB290637)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (AB290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168588.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (AB290637)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (AB290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165446.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (AB290637)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (AB290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172296.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (AB290637)")
Publications
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- Journal of food biochemistry 46:e145002022Laminarin ameliorates alcohol-induced liver damage and its molecular mechanism in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianyi Guo,Lingfeng Zhu,Yaping Zhou,Shuai Han,Yunyun Cao,Zuomin Hu,Yi Luo,Liyuan Bao,Xiuxiu Wu,Dandan Qin,Qinlu Lin,Feijun LuoPubMed 36515171
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Tested | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Alternative names
Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Afp
Recommended products
Anti-alpha 1 Fetoprotein antibody [EPR26548-19] is a Rabbit Monoclonal antibody that is used in alpha 1 Fetoprotein IHC-P, Western Blot. Suitable for Mouse, Rat samples.
Alpha-fetoprotein (AFP) is a key biomarker for diagnosing hepatocellular carcinoma (HCC), with elevated levels indicating liver cancer. It contributes to an immune-tolerant tumor microenvironment, helping cancer cells evade immune detection. AFP is being explored as a therapeutic target for liver cancer using CAR-T cells but the major focus is using AFP as a diagnostic or prognostic marker for liver, colorectal and gastric cancers.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26548-19
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.
- Biological function summary
Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.
- Pathways
Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.
- Associated diseases and disorders
High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--western-blot-img167073.jpg "Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)")
Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)
Blocking and dilution buffer and concentration was 5% NFDM/TBST.
All lanes: Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637) at 1000 Cells
Lane 1: Untreated Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Hepa1-6 treated with 300ng/ml Brefeldin A for 24h whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 68 kDa
Observed band size: 70 kDa
Exposure time: 37s
![Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--western-blot-img170128.jpg "Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)")
Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)
Blocking and dilution buffer and concentration was 5% NFDM/TBST.
Negative control: liver, brain(PMID: 16708394, PMID: 9891062).
All lanes: Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637) at 1/1000 dilution
Lane 1: Mouse E14 embryo tissue lysate at 20 µg
Lane 2: Mouse placenta tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Mouse brain tissue lysate at 20 µg
Lane 5: Rat placenta tissue lysate at 20 µg
Lane 6: Rat liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 68 kDa
Observed band size: 70 kDa
Exposure time: 8s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168588.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)
Immunohistochemical analysis of paraffin-embedded Mouse liver E16.5 tissue labelling alpha 1 Fetoprotein with ab290637 at 1/1000 (0.534 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse fetal liver (E16.5) (PMID: 15005571). The section was incubated with ab290637 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165446.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue labelling alpha 1 Fetoprotein with ab290637 at 1/1000 (0.534 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining was found on mouse placenta. The section was incubated with ab290637 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172296.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)
Immunohistochemical analysis of paraffin-embedded rat placenta tissue labelling alpha 1 Fetoprotein with ab290637 at 1/1000 (0.534 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining was found on rat placenta. The section was incubated with ab290637 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176362.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling alpha 1 Fetoprotein with ab290637 at 1/1000 (0.534 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on adult mouse liver. The section was incubated with ab290637 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176891.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labelling alpha 1 Fetoprotein with ab290637 at 1/1000 (0.534 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on adult rat liver. The section was incubated with ab290637 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176362.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr26548-19-ab290637--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176891.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637)")
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