Rabbit Recombinant Monoclonal alpha 1 Fetoprotein antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (AB188828), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr9309-bsa-and-azide-free-ab188828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118970.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (AB188828)")
![Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (AB188828), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr9309-bsa-and-azide-free-ab188828--immunoprecipitation-img86574.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (AB188828)")
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (AB188828), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr9309-bsa-and-azide-free-ab188828--immunocytochemistry-immunofluorescence-img19956.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (AB188828)")
![Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (AB188828), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr9309-bsa-and-azide-free-ab188828--flow-cytometry-intracellular-img137668.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (AB188828)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (AB188828), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr9309-bsa-and-azide-free-ab188828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40639.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (AB188828)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376-Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
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1 product for Alternative Version
Target data
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Alternative names
HPAFP, AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin
Recommended products
Rabbit Recombinant Monoclonal alpha 1 Fetoprotein antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9309
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab188828 is the carrier-free version of Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.
- Biological function summary
Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.
- Pathways
Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.
- Associated diseases and disorders
High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr9309-bsa-and-azide-free-ab188828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118970.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828)
Immunohistochemical analysis of paraffin embedded human moderately differentiated hepatocellular carcinoma tissue labeling alpha 1 Fetoprotein with Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552 antibody at 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr9309-bsa-and-azide-free-ab188828--immunoprecipitation-img86574.jpg "Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828)")
Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828)
Immunoprecipitation. Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552 at 1/1000 staining alpha 1 Fetoprotein in HepG2 cell lysate immunoprecipitated using Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552 at 1/10. Predicted band size : 68 kDa.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552).
All lanes: Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR9309] (Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552)
Predicted band size: 68 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr9309-bsa-and-azide-free-ab188828--immunocytochemistry-immunofluorescence-img19956.jpg "Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828)")
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828)
Immunofluorescent analysis of HepG2 cells labeling alpha 1 Fetoprotein with Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552 at 1/100. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552).
![Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr9309-bsa-and-azide-free-ab188828--flow-cytometry-intracellular-img137668.jpg "Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828)")
Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828)
Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling alpha 1 Fetoprotein with purified Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552 at 1/50 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-fetoprotein-antibody-epr9309-bsa-and-azide-free-ab188828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40639.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] - BSA and Azide free (ab188828)
Immunohistochemical analysis of paraffin embedded Human poorly differentiated hepatocellular carcinoma tissue labeling alpha 1 Fetoprotein with Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552 antibody at 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha 1 Fetoprotein antibody [EPR9309] ab169552).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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