AB130748

Anti-alpha 1 Fetoprotein antibody [SP154]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal alpha 1 Fetoprotein antibody. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

HPAFP, AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [SP154] (AB130748)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [SP154] (AB130748)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling alpha 1 Fetoprotein with ab130748 at 1/50 dilution (4.98 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [SP154] (AB130748)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [SP154] (AB130748)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling alpha 1 Fetoprotein with purified ab130748 at 1 : 100 (2.5 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [SP154] (AB130748)

Flow Cyt

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Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [SP154] (AB130748)

Flow Cytometry analysis of HepG2(Human hepatocellular carcinoma epithelial cell) cells labeling alpha 1 Fetoprotein with purified ab130748 at 1 : 240 dilution (1.04 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.

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Western blot - Anti-alpha 1 Fetoprotein antibody [SP154] (AB130748)

All lanes:

Western blot - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748) at 1/100 dilution

All lanes:

HepG2 cell lysate

Predicted band size: 68 kDa

Observed band size: 70 kDa

false

Carrier free

Anti-alpha 1 Fetoprotein antibody [SP154] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP154

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "Incubate for 10 minutes at room temperature.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/240", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "Incubate for 1 hour at room temperature.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.

Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

See full target information AFP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 24:411 PubMed36245818

2022

Individualized therapy based on the combination of mini-PDX and NGS for a patient with metastatic AFP-producing and HER-2 amplified gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodan Zhu,Xiaoqing Xu,Baonan Zhang,Yun Dong,Shixia Gong,Tingjie Gong,Feifei Zhang,Chunhui Jin

The Journal of experimental medicine 218: PubMed33851955

2021

Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph E Rupert,Ashok Narasimhan,Daenique H A Jengelley,Yanlin Jiang,Jianguo Liu,Ernie Au,Libbie M Silverman,George Sandusky,Andrea Bonetto,Sha Cao,Xiaoyu Lu,Thomas M O'Connell,Yunlong Liu,Leonidas G Koniaris,Teresa A Zimmers

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Fetoprotein antibody [SP154]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [SP154] (AB130748)

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [SP154] (AB130748)

Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [SP154] (AB130748)

Western blot - Anti-alpha 1 Fetoprotein antibody [SP154] (AB130748)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise