AB228541

Anti-alpha 1 Glycine Receptor/GLRA1 antibody

Name: Anti-alpha 1 Glycine Receptor/GLRA1 antibody (ab228541) | Abcam
Brand: Abcam Limited
SKU: AB228541
Price: 525 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal alpha 1 Glycine Receptor/GLRA1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLRA1.

View Alternative Names

Glycine receptor subunit alpha-1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit, GLRA1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Glycine Receptor/GLRA1 antibody (AB228541)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Glycine Receptor/GLRA1 antibody (AB228541)

Paraffin-embedded OVCA (human) xenograft tissue stained for alpha 1 Glycine Receptor/GLRA1 using ab228541 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Glycine Receptor/GLRA1 antibody (AB228541)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Glycine Receptor/GLRA1 antibody (AB228541)

4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neuron cells labeling alpha 1 Glycine Receptor/GLRA1 using ab228541 at 1/500 dilution.

The nuclear counterstain is DAPI (blue). beta Tubulin is labeled with an anti-beta Tubulin antibody (red).

Supplier Data

Western blot - Anti-alpha 1 Glycine Receptor/GLRA1 antibody (AB228541)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-alpha 1 Glycine Receptor/GLRA1 antibody (ab228541) at 1/1500 dilution

All lanes:

HeLa S3 whole cell lysate at 30 µg

Predicted band size: 53 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GLRA1. The exact immunogen used to generate this antibody is proprietary information.

P23415

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The alpha 1 Glycine Receptor also known as GLRA1 is a ligand-gated ion channel receptor. It plays an important role in transmitting inhibitory neurotransmission by binding glycine a neurotransmitter. The receptor possesses a mass around 48 kDa and is mainly expressed in the central nervous system particularly in the spinal cord and brainstem. This receptor helps regulate chloride ion influx which stabilizes neuronal membrane potential by reducing the likelihood of neuron firing.

Biological function summary

The GLRA1 facilitates inhibitory synapses in the central nervous system. Working as part of a larger receptor complex it couples with glycine to enable chloride anion flow across the neuronal membrane. This results in hyperpolarization which decreases the neuronal excitability. The receptor operates within a pentameric configuration often interacting with other subunits like beta glycine receptor subunits to form heteromeric channels.

Pathways

The GLRA1 is essential in the glycinergic pathway which serves to modulate excitatory signals in the nervous system by providing inhibitory control. It closely relates to other ligand-gated ion channels such as the GABAA receptor which shares a similar inhibitory function. The proper functioning of GLRA1 ensures balance in neurotransmission and prevents excessive neuronal firing which can lead to disorders.

Dysfunctional GLRA1 contributes to hyperekplexia also known as startle disease a genetic neurological disorder affecting muscle control. Mutations in the GLRA1 affect its ability to bind glycine effectively leading to impaired inhibitory neurotransmission. This receptor also shows a connection to disorders involving neurotransmission imbalance when linked with the protein gephyrin which anchors and clusters the glycine receptors at synaptic sites to ensure proper inhibitory signaling.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Subunit of heteromeric glycine-gated chloride channels (PubMed : 14551753, PubMed : 23994010, PubMed : 25730860, PubMed : 37821459). Plays an important role in the down-regulation of neuronal excitability (PubMed : 8298642, PubMed : 9009272). Contributes to the generation of inhibitory postsynaptic currents (PubMed : 25445488). Channel activity is potentiated by ethanol (PubMed : 25973519). Potentiation of channel activity by intoxicating levels of ethanol contribute to the sedative effects of ethanol (By similarity).

See full target information GLRA1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw9952 PubMed40864703

2025

Mitochondrial ROS drive foam cell formation via STAT5 signaling in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Boccuni,Frieda Marka,Manuel Salzmann,Alessia Schirripa,Elisabeth Ableitner,Magdalena Siller,Mira Brekalo,Patrick Haider,Stefan Stojkovic,Christoph Neumayer,Tiit Örd,Karoline Kollmann,Alice Assinger,Thomas Decker,Thomas Köcher,Michael B Fischer,Marion Mußbacher,Andreas Bergthaler,Christian Hengstenberg,Bruno K Podesser,Minna U Kaikkonen,Johann Wojta,Philipp J Hohensinner

iScience 25:105366 PubMed36339258

2022

Single-cell RNA-seq transcriptomic landscape of human and mouse islets and pathological alterations of diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Chen,Junqing Zhang,Youyuan Huang,Xiaodong Tian,Yinmo Yang,Aimei Dong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Glycine Receptor/GLRA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Glycine Receptor/GLRA1 antibody (AB228541)

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Glycine Receptor/GLRA1 antibody (AB228541)

Western blot - Anti-alpha 1 Glycine Receptor/GLRA1 antibody (AB228541)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise