AB11751

Anti-alpha 1 Spectrin antibody [17C7]

Name: Anti-alpha 1 Spectrin antibody [17C7] (ab11751) | Abcam
Brand: Abcam Limited
SKU: AB11751
Rating: 4 (3 reviews)

(3 Reviews)

(17 Publications)

Anti-alpha 1 Spectrin antibody [17C7] (ab11751) is a mouse monoclonal antibody detecting alpha 1 Spectrin in Western Blot, IHC-Fr, ICC/IF, ELISA. Suitable for Human, Mouse, Rat.

- Over 10 publications
- Trusted since 2004

View Alternative Names

SPTA, SPTA1, Erythroid alpha-spectrin

2 Images

Supplier Data

Western blot - Anti-alpha 1 Spectrin antibody [17C7] (AB11751)

All lanes:

Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1 µg/mL

Lane 1:

MW marker at 50 µg

Lane 2:

Human Lung lysate at 50 µg

Lane 3:

Mouse Lung lysate at 50 µg

Lane 4:

Rat Brain lysate at 50 µg

Lane 5:

Human Kidney lysate at 50 µg

Secondary

All lanes:

HRP labeled goat anti-mouse IgG

Predicted band size: 280 kDa

false

AbReview17118****

Western blot - Anti-alpha 1 Spectrin antibody [17C7] (AB11751)

All lanes:

Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1/1000 dilution

All lanes:

Human Platelet whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated Goat Anti-mouse IgG at 1/10000 dilution

Predicted band size: 280 kDa

Observed band size: 200 kDa

true

Exposure time: 1hr

This image is courtesy of an Abreview submitted by Dr. Mahesh Shivananjappa

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

17C7

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-Fr, WB, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SPTA1.

P02549

Specificity

ab11751 is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, ab11751 reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-alpha 1 Spectrin antibody [17C7] (ab11751) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Mouse, Rat samples.

What is the molecular weight of alpha 1 Spectrin?
Anti-alpha 1 Spectrin [17C7] (ab11751) specifically detects a band for alpha 1 Spectrin (UniProt: P02549) at a molecular weight of 280kDa.

Trusted by the scientific community
Anti-alpha 1 Spectrin [17C7] (ab11751) was first used in a scientific publication in 2004 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Spectrin also known as SPTA1 is an essential protein that plays a mechanical role in cells. This protein has a large molecular mass of approximately 280 kDa and is predominantly expressed in erythrocytes but it is also found in other cell types. Structurally it forms part of the spectrin family which includes alpha and beta spectrin subunits. Alpha 1 Spectrin interacts with the actin cytoskeleton creating a network that supports the plasma membrane’s integrity and flexibility.

Biological function summary

Alpha 1 Spectrin binds with other proteins to form a spectrin-actin complex that is essential for maintaining cell shape and mechanical stability. It interacts with ankyrin and other components of the cytoskeleton to provide the necessary elasticity and durability to cells especially red blood cells. This protein makes sure that the cytoskeletal network remains stable and efficiently responds to mechanical stresses from the outside environment.

Pathways

Alpha 1 Spectrin is involved in critical pathways important for cellular functions. It participates in the erythrocyte cytoskeleton organization pathway interacting with proteins such as ankyrin and protein 4.1R. These interactions influence cell deformability and help maintain the biconcave shape of red blood cells allowing them to navigate through narrow capillaries effectively. Alpha 1 Spectrin’s functionality ensures efficient oxygen delivery throughout the body by maintaining red blood cell integrity.

Mutations or deficiencies in Alpha 1 Spectrin can lead to conditions such as hereditary spherocytosis and elliptocytosis. These disorders are characterized by hemolytic anemia and are linked to disruptions in the cytoskeleton structure and function of erythrocytes. In these cases Alpha 1 Spectrin can display abnormal interactions with related proteins like ankyrin leading to compromised membrane stability and shortened red blood cell lifespan. The understanding of Alpha 1 Spectrin’s role in these diseases provides insight into potential therapeutic targets for managing these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.

See full target information SPTA1

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology 602:1127-1145 PubMed38441922

2024

Postsynaptic β1 spectrin maintains Na channels at the neuromuscular junction.

Applications

Unspecified application

Species

Unspecified reactive species

Ozlem Sert,Xiaoyun Ding,Chuansheng Zhang,Ruifa Mi,Ahmet Hoke,Matthew N Rasband

iScience 26:105725 PubMed36579133

2022

Ring-stage growth arrest: Metabolic basis of artemisinin tolerance in .

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Yu,Changhong Wang,Yuemeng Zhao,Jianxia Tang,Meng Zhu,Lucien Platon,Richard Culleton,Guoding Zhu,Didier Ménard,Qingfeng Zhang,Jun Cao

Andrology 11:591-610 PubMed36374586

2022

Cytoskeletal protein SPTA1 mediating the decrease in erectile function induced by high-fat diet via Hippo signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Lei Wang,Zhan-Sen Huang,Jia-Xin Feng,Shi-Xiong Li,Zi-Jun Du,Ze-Bo Zhang,Jing Liu,Jian Yang,Zhi-Ming Hu,Zhi-Lin Wang,Jun Chen

International journal of molecular sciences 23: PubMed35008585

2021

STIM1 Controls the Focal Adhesion Dynamics and Cell Migration by Regulating SOCE in Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Shan Lin,Yi-Hsin Lin,MyHang Nguyen Thi,Shih-Chuan Hsiao,Wen-Tai Chiu

Nature communications 12:1172 PubMed33608523

2021

20S proteasomes secreted by the malaria parasite promote its growth.

Applications

Unspecified application

Species

Unspecified reactive species

Elya Dekel,Dana Yaffe,Irit Rosenhek-Goldian,Gili Ben-Nissan,Yifat Ofir-Birin,Mattia I Morandi,Tamar Ziv,Xavier Sisquella,Matthew A Pimentel,Thomas Nebl,Eugene Kapp,Yael Ohana Daniel,Paula Abou Karam,Daniel Alfandari,Ron Rotkopf,Shimrit Malihi,Tal Block Temin,Debakshi Mullick,Or-Yam Revach,Ariel Rudik,Nir S Gov,Ido Azuri,Ziv Porat,Giulia Bergamaschi,Raya Sorkin,Gijs J L Wuite,Ori Avinoam,Teresa G Carvalho,Sidney R Cohen,Michal Sharon,Neta Regev-Rudzki

American journal of hematology 94:667-677 PubMed30916803

2019

MYH9-related disease mutations cause abnormal red blood cell morphology through increased myosin-actin binding at the membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Alyson S Smith,Kasturi Pal,Roberta B Nowak,Anastasiya Demenko,Carlo Zaninetti,Lydie Da Costa,Remi Favier,Alessandro Pecci,Velia M Fowler

Cell metabolism 28:400-414.e8 PubMed30017354

2018

Mitofusin 2 Regulates Axonal Transport of Calpastatin to Prevent Neuromuscular Synaptic Elimination in Skeletal Muscles.

Applications

Unspecified application

Species

Unspecified reactive species

Luwen Wang,Ju Gao,Jingyi Liu,Sandra L Siedlak,Sandy Torres,Hisashi Fujioka,Mikayla L Huntley,Yinfei Jiang,Haiyan Ji,Tingxiang Yan,Micah Harland,Pichet Termsarasab,Sophia Zeng,Zhen Jiang,Jingjing Liang,George Perry,Charles Hoppel,Cheng Zhang,Hu Li,Xinglong Wang

The Journal of clinical investigation 128:760-773 PubMed29337302

2018

Critical roles of αII spectrin in brain development and epileptic encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Tuo Ji,Andrew D Nelson,Katarzyna Glanowska,Geoffrey G Murphy,Paul M Jenkins,Jack M Parent

Biochimica et biophysica acta. Biomembranes 1859:2381-2391 PubMed28919343

2017

Characterizing pathology in erythrocytes using morphological and biophysical membrane properties: Relation to impaired hemorheology and cardiovascular function in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Oore-Ofe O Olumuyiwa-Akeredolu,Prashilla Soma,Antoinette V Buys,Legesse Kassa Debusho,Etheresia Pretorius

G3 (Bethesda, Md.) 7:3133-3144 PubMed28751503

2017

Ankyrin-1 Gene Exhibits Allelic Heterogeneity in Conferring Protection Against Malaria.

Applications

Species

Mouse

Hong Ming Huang,Denis C Bauer,Patrick M Lelliott,Matthew W A Dixon,Leann Tilley,Brendan J McMorran,Simon J Foote,Gaetan Burgio

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Spectrin antibody [17C7]

Western blot - Anti-alpha 1 Spectrin antibody [17C7] (AB11751)

Western blot - Anti-alpha 1 Spectrin antibody [17C7] (AB11751)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise