AB154811

Anti-alpha 1 Spectrin antibody [EPR9299]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal alpha 1 Spectrin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SPTA, SPTA1, Erythroid alpha-spectrin

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling alpha 1 Spectrin with ab154811 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

Immunohistochemical analysis of paraffin embedded Human normal liver erythrocytes tissue using ab154811 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

Immunohistochemical analysis of paraffin embedded Human normal spleen erythrocytes tissue using ab154811 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

Immunohistochemical analysis of paraffin embedded Human placenta erythrocytes tissue using ab154811 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling alpha 1 Spectrin with ab154811 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

Immunohistochemical analysis of paraffin embedded Human normal uterus, erythrocytes tissue using ab154811 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

WB

Unknown

Western blot - Anti-alpha 1 Spectrin antibody [EPR9299] (AB154811)

All lanes:

Western blot - Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

TF-1 cell lysate at 10 µg

Predicted band size: 171 kDa,280 kDa,47 kDa,57 kDa,60 kDa,62 kDa

Observed band size: 54 kDa,60 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9299

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Spectrin also known as SPTA1 is an essential protein that plays a mechanical role in cells. This protein has a large molecular mass of approximately 280 kDa and is predominantly expressed in erythrocytes but it is also found in other cell types. Structurally it forms part of the spectrin family which includes alpha and beta spectrin subunits. Alpha 1 Spectrin interacts with the actin cytoskeleton creating a network that supports the plasma membrane’s integrity and flexibility.

Biological function summary

Alpha 1 Spectrin binds with other proteins to form a spectrin-actin complex that is essential for maintaining cell shape and mechanical stability. It interacts with ankyrin and other components of the cytoskeleton to provide the necessary elasticity and durability to cells especially red blood cells. This protein makes sure that the cytoskeletal network remains stable and efficiently responds to mechanical stresses from the outside environment.

Pathways

Alpha 1 Spectrin is involved in critical pathways important for cellular functions. It participates in the erythrocyte cytoskeleton organization pathway interacting with proteins such as ankyrin and protein 4.1R. These interactions influence cell deformability and help maintain the biconcave shape of red blood cells allowing them to navigate through narrow capillaries effectively. Alpha 1 Spectrin’s functionality ensures efficient oxygen delivery throughout the body by maintaining red blood cell integrity.

Mutations or deficiencies in Alpha 1 Spectrin can lead to conditions such as hereditary spherocytosis and elliptocytosis. These disorders are characterized by hemolytic anemia and are linked to disruptions in the cytoskeleton structure and function of erythrocytes. In these cases Alpha 1 Spectrin can display abnormal interactions with related proteins like ankyrin leading to compromised membrane stability and shortened red blood cell lifespan. The understanding of Alpha 1 Spectrin’s role in these diseases provides insight into potential therapeutic targets for managing these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.

See full target information SPTA1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35008858

2021

New Proteins Contributing to Immune Cell Infiltration and Pannus Formation of Synovial Membrane from Arthritis Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique de Seny,Dominique Baiwir,Elettra Bianchi,Gaël Cobraiville,Céline Deroyer,Christophe Poulet,Olivier Malaise,Geneviève Paulissen,Marie-Joëlle Kaiser,Jean-Philippe Hauzeur,Gabriel Mazzucchelli,Philippe Delvenne,Michel Malaise

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com