AB139403

Anti-alpha 1 Spectrin antibody [EPR9300]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal alpha 1 Spectrin antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

SPTA, SPTA1, Erythroid alpha-spectrin

2 Images

Flow Cytometry (Intracellular) - Anti-alpha 1 Spectrin antibody [EPR9300] (AB139403)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-alpha 1 Spectrin antibody [EPR9300] (AB139403)

Intracellular flow cytometric analysis of permeabilized K562 cells labelling alpha 1 Spectrin with ab139403 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

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Western blot - Anti-alpha 1 Spectrin antibody [EPR9300] (AB139403)

All lanes:

Western blot - Anti-alpha 1 Spectrin antibody [EPR9300] (ab139403) at 1/1000 dilution

Lane 1:

K562 lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 280 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9300

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Spectrin also known as SPTA1 is an essential protein that plays a mechanical role in cells. This protein has a large molecular mass of approximately 280 kDa and is predominantly expressed in erythrocytes but it is also found in other cell types. Structurally it forms part of the spectrin family which includes alpha and beta spectrin subunits. Alpha 1 Spectrin interacts with the actin cytoskeleton creating a network that supports the plasma membrane’s integrity and flexibility.

Biological function summary

Alpha 1 Spectrin binds with other proteins to form a spectrin-actin complex that is essential for maintaining cell shape and mechanical stability. It interacts with ankyrin and other components of the cytoskeleton to provide the necessary elasticity and durability to cells especially red blood cells. This protein makes sure that the cytoskeletal network remains stable and efficiently responds to mechanical stresses from the outside environment.

Pathways

Alpha 1 Spectrin is involved in critical pathways important for cellular functions. It participates in the erythrocyte cytoskeleton organization pathway interacting with proteins such as ankyrin and protein 4.1R. These interactions influence cell deformability and help maintain the biconcave shape of red blood cells allowing them to navigate through narrow capillaries effectively. Alpha 1 Spectrin’s functionality ensures efficient oxygen delivery throughout the body by maintaining red blood cell integrity.

Mutations or deficiencies in Alpha 1 Spectrin can lead to conditions such as hereditary spherocytosis and elliptocytosis. These disorders are characterized by hemolytic anemia and are linked to disruptions in the cytoskeleton structure and function of erythrocytes. In these cases Alpha 1 Spectrin can display abnormal interactions with related proteins like ankyrin leading to compromised membrane stability and shortened red blood cell lifespan. The understanding of Alpha 1 Spectrin’s role in these diseases provides insight into potential therapeutic targets for managing these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.

See full target information SPTA1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomarkers in medicine 13:1209-1225 PubMed31379197

2019

Orthogonal analysis of dystrophin protein and mRNA as a surrogate outcome for drug development.

Applications

Unspecified application

Species

Unspecified reactive species

Kitipong Uaesoontrachoon,Sadish Srinivassane,Jordan Warford,Kevork Mekhssian,Hélène Montpetit,Romain Beauvois,Anahita Keyhani,Yetrib Hathout,Taishi Yamashita,Youhei Satou,Hironori Osaki,Molly Praest,Marina Moraca,Maja Malbasic,William Ross,Alexandra MacKinnon,Joyce Rowsell,Amanda Mullen,Mark Matyas,Swati Mummidivarpu,Kanneboyina Nagaraju,Eric P Hoffman

Nature communications 9:4960 PubMed30470753

2018

The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Daan Vorselen,Susan M van Dommelen,Raya Sorkin,Melissa C Piontek,Jürgen Schiller,Sander T Döpp,Sander A A Kooijmans,Brigitte A van Oirschot,Birgitta A Versluijs,Marc B Bierings,Richard van Wijk,Raymond M Schiffelers,Gijs J L Wuite,Wouter H Roos

Nanoscale 10:2413-2426 PubMed29334397

2018

Recombinant phosphatidylserine-binding nanobodies for targeting of extracellular vesicles to tumor cells: a plug-and-play approach.

Applications

Unspecified application

Species

Unspecified reactive species

Sander A A Kooijmans,Jerney J J M Gitz-Francois,Raymond M Schiffelers,Pieter Vader

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Spectrin antibody [EPR9300]

Flow Cytometry (Intracellular) - Anti-alpha 1 Spectrin antibody [EPR9300] (AB139403)

Western blot - Anti-alpha 1 Spectrin antibody [EPR9300] (AB139403)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise