AB140613

Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)]

Name: Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] (ab140613) | Abcam
Brand: Abcam Limited
SKU: AB140613
Price: 365 USD
Availability: InStock
Rating: 5 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(7 Publications)

Rabbit Recombinant Monoclonal alpha 1,2 Mannosidase IA/MAN1A1 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Man(9)-alpha-mannosidase, Mannosidase alpha class 1A member 1, Man9-mannosidase, MAN1A1

1 Images

Unknown

Western blot - Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] (AB140613)

All lanes:

Western blot - Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] (ab140613) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Carrier free

Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9957(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 12 Mannosidase IA also known as MAN1A1 is an enzyme that catalyzes the hydrolysis of terminal α-D-mannose residues in the N-glycan processing pathway. This enzyme functions as a mannosyl-oligosaccharide 12-alpha-mannosidase and its molecular mass is approximately 73 kDa. MAN1A1 is expressed in various tissues including high expression levels in the liver and kidney. The enzyme has a significant role in the Golgi apparatus of cells where it contributes to glycoprotein maturation by trimming mannose residues.

Biological function summary

Alpha 12 Mannosidase IA influences glycoprotein quality control by processing N-linked glycans. As part of the glycosylation machinery this enzyme ensures proper protein folding and stability. MAN1A1 does not directly form part of a multiprotein complex but interacts with various substrates during the glycan trimming process. This interaction helps in determining the fate of glycoproteins and their proper cellular localization.

Pathways

Alpha 12 Mannosidase IA plays an important role in the N-glycan biosynthesis and processing pathway where it alters the structure of N-glycans by removing mannose residues. This enzyme is connected to the calnexin/calreticulin cycle an important element of glycoprotein maturation. Related proteins such as glucosidase II further process the glycoproteins as they mature in the Golgi apparatus. This sequential processing is essential for forming high-mannose glycans and complex N-glycans that are important for protein function.

Alpha 12 Mannosidase IA has associations with congenital disorders of glycosylation (CDG) a group of diseases impacting glycoprotein synthesis and processing. Abnormal MAN1A1 activity can lead to improper glycoprotein folding causing a variety of systemic symptoms. Additionally altered MAN1A1 expression has been observed in certain types of cancer influencing tumor progression due to changes in cell surface glycosylation patterns. MAN1A1 activity closely links with other enzymes in the N-glycan maturation pathway like α-mannosidase II which further highlights its impact on glycosylation-related diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).

See full target information MAN1A1

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 41:111679 PubMed36417860

2022

Mitotic phosphorylation inhibits the Golgi mannosidase MAN1A1.

Applications

Unspecified application

Species

Unspecified reactive species

Shijiao Huang,Yoshimi Haga,Jie Li,Jianchao Zhang,Hye Kyong Kweon,Junichi Seino,Hiroto Hirayama,Morihisa Fujita,Kelley W Moremen,Philip Andrews,Tadashi Suzuki,Yanzhuang Wang

Frontiers in pharmacology 12:805499 PubMed35002739

2021

Elevated N-Glycosylation Contributes to the Cisplatin Resistance of Non-Small Cell Lung Cancer Cells Revealed by Membrane Proteomic and Glycoproteomic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Zeng,Shanshan Zheng,Yonghong Mao,Shisheng Wang,Yi Zhong,Wei Cao,Tao Su,Meng Gong,Jingqiu Cheng,Yong Zhang,Hao Yang

Biochemical and biophysical research communications 527:406-410 PubMed32331836

2020

Markers of malignant prostate cancer cells: Golgi localization of α-mannosidase 1A at GM130-GRASP65 site and appearance of high mannose N-glycans on cell surface.

Applications

Unspecified application

Species

Unspecified reactive species

Pi-Wan Cheng,Samuel Davidson,Ganapati Bhat

Cells 8: PubMed31847122

2019

Post-ER Stress Biogenesis of Golgi Is Governed by Giantin.

Applications

Unspecified application

Species

Unspecified reactive species

Cole P Frisbie,Alexander Y Lushnikov,Alexey V Krasnoslobodtsev,Jean-Jack M Riethoven,Jennifer L Clarke,Elena I Stepchenkova,Armen Petrosyan

Autophagy 15:1787-1800 PubMed30894053

2019

GORASP2/GRASP55 collaborates with the PtdIns3K UVRAG complex to facilitate autophagosome-lysosome fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Zhang,Leibin Wang,Stephen C Ireland,Erpan Ahat,Jie Li,Michael E Bekier,Zhihai Zhang,Yanzhuang Wang

British journal of cancer 118:847-856 PubMed29381688

2018

Reduced mannosidase MAN1A1 expression leads to aberrant N-glycosylation and impaired survival in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Legler,Ricarda Rosprim,Tosca Karius,Kathrin Eylmann,Maila Rossberg,Ralph M Wirtz,Volkmar Müller,Isabell Witzel,Barbara Schmalfeldt,Karin Milde-Langosch,Leticia Oliveira-Ferrer

Alcoholism, clinical and experimental research 40:2573-2590 PubMed27748959

2016

Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Carol A Casey,Ganapati Bhat,Melissa S Holzapfel,Armen Petrosyan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)]

Western blot - Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] (AB140613)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise