AB248903

Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal alpha 1,2 Mannosidase IA/MAN1A1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Man(9)-alpha-mannosidase, Mannosidase alpha class 1A member 1, Man9-mannosidase, MAN1A1

1 Images

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Western blot - Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] - BSA and Azide free (AB248903)

This data was developed using ab140613, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] (<a href='/en-us/products/primary-antibodies/alpha-12-mannosidase-ia-man1a1-antibody-epr9957b-ab140613'>ab140613</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9957(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab248903 is the carrier-free version of ab140613.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 12 Mannosidase IA also known as MAN1A1 is an enzyme that catalyzes the hydrolysis of terminal α-D-mannose residues in the N-glycan processing pathway. This enzyme functions as a mannosyl-oligosaccharide 12-alpha-mannosidase and its molecular mass is approximately 73 kDa. MAN1A1 is expressed in various tissues including high expression levels in the liver and kidney. The enzyme has a significant role in the Golgi apparatus of cells where it contributes to glycoprotein maturation by trimming mannose residues.

Biological function summary

Alpha 12 Mannosidase IA influences glycoprotein quality control by processing N-linked glycans. As part of the glycosylation machinery this enzyme ensures proper protein folding and stability. MAN1A1 does not directly form part of a multiprotein complex but interacts with various substrates during the glycan trimming process. This interaction helps in determining the fate of glycoproteins and their proper cellular localization.

Pathways

Alpha 12 Mannosidase IA plays an important role in the N-glycan biosynthesis and processing pathway where it alters the structure of N-glycans by removing mannose residues. This enzyme is connected to the calnexin/calreticulin cycle an important element of glycoprotein maturation. Related proteins such as glucosidase II further process the glycoproteins as they mature in the Golgi apparatus. This sequential processing is essential for forming high-mannose glycans and complex N-glycans that are important for protein function.

Alpha 12 Mannosidase IA has associations with congenital disorders of glycosylation (CDG) a group of diseases impacting glycoprotein synthesis and processing. Abnormal MAN1A1 activity can lead to improper glycoprotein folding causing a variety of systemic symptoms. Additionally altered MAN1A1 expression has been observed in certain types of cancer influencing tumor progression due to changes in cell surface glycosylation patterns. MAN1A1 activity closely links with other enzymes in the N-glycan maturation pathway like α-mannosidase II which further highlights its impact on glycosylation-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).

See full target information MAN1A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] - BSA and Azide free

Western blot - Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] - BSA and Azide free (AB248903)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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