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AB137123

Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)]

5

(1 Review)

|

(28 Publications)

Rabbit Recombinant Monoclonal alpha 1a Adrenergic Receptor/ADRA1A antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 28 publications.

View Alternative Names

ADRA1C, ADRA1A, Alpha-1A adrenergic receptor, Alpha-1A adrenoreceptor, Alpha-1C adrenergic receptor, Alpha-adrenergic receptor 1c, Alpha-1A adrenoceptor

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (AB137123)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (AB137123)

Immunofluorescent analysis of HepG2 cells labelling alpha 1a Adrenergic Receptor/ADRA1A with ab137123 at 1/250 dilution.

Flow Cytometry (Intracellular) - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (AB137123)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (AB137123)

Overlay histogram showing HepG2 cells stained with ab137123 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137123, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Western blot - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (AB137123)
  • WB

Unknown

Western blot - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (AB137123)

All lanes:

Western blot - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123) at 1/1000 dilution

Lane 1:

PC-3 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 52 kDa

false

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)]

  • Carrier free

    Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] - BSA and Azide free

  • 578 PE

    PE Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9691(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The alpha 1a Adrenergic Receptor also known as ADRA1A or alpha 1a receptor is a type of G protein-coupled receptor with an approximate mass of 56 kDa. It is a part of the adrenergic receptors family which are known for responding to adrenaline and noradrenaline. This receptor is expressed predominantly in smooth muscle tissues such as those in the prostate and blood vessels. Mechanically the A1 adrenergic receptor activates phospholipase C via Gq proteins leading to an increase in intracellular calcium levels.
Biological function summary

Alpha 1a Adrenergic Receptor plays a critical role in regulating smooth muscle contraction and blood vessel vasoconstriction. It forms an important part of the adrenergic receptor system which also includes alpha adrenergic receptors such as alpha 1b and alpha 1d subclasses. The activation of the alpha 1a receptor promotes vasoconstriction which contributes to blood pressure regulation. Additionally it is involved in modulating neurotransmitter release through its presence in nerve cells.

Pathways

Alpha 1a Adrenergic Receptor figures prominently in signaling pathways that manage cardiovascular functions. It is tightly associated with the GPCR signaling pathway which includes other adrenergic receptors and proteins like beta adrenergic receptors. Another significant pathway is the calcium signaling pathway where its impact on intracellular calcium plays a role in muscle contractions linking it to other proteins involved in the contraction machinery.

Alpha 1a Adrenergic Receptor connects to conditions such as hypertension and benign prostatic hyperplasia (BPH). The receptor’s role in vasoconstriction directly links to hypertension where it affects arterial blood pressure. In BPH the alpha 1a receptor influences prostate smooth muscle tone making it a target in treating urinary symptoms. These conditions involve proteins like angiotensin-converting enzyme in hypertension and other alpha receptors in BPH highlighting the ADRA1A's importance in therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.
See full target information ADRA1A
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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed36831252

2023

Alpha1A- and Beta3-Adrenoceptors Interplay in Adipose Multipotent Mesenchymal Stromal Cells: A Novel Mechanism of Obesity-Driven Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Vadim Chechekhin,Anastasia Ivanova,Konstantin Kulebyakin,Veronika Sysoeva,Daria Naida,Mikhail Arbatsky,Nataliya Basalova,Maxim Karagyaur,Mariya Skryabina,Anastasia Efimenko,Olga Grigorieva,Natalia Kalinina,Vsevolod Tkachuk,Pyotr Tyurin-Kuzmin

American journal of physiology. Heart and circulatory physiology 324:H417-H429 PubMed36705993

2023

Carotid dysfunction in senescent female mice is mediated by increased α-adrenoceptor activity and COX-derived vasoconstrictor prostanoids.

Applications

Unspecified application

Species

Unspecified reactive species

Tiago J Costa,Paula R Barros,Diego A Duarte,Júlio A Silva-Neto,Sara Cristina Hott,Thamyris Santos-Silva,Claudio M Costa-Neto,Felipe V Gomes,Eliana H Akamine,Cameron G McCarthy,Francesc Jimenez-Altayó,Ana Paula Dantas,Rita C Tostes

American journal of physiology. Lung cellular and molecular physiology 324:L639-L651 PubMed36648147

2023

α1 Adrenoreceptor antagonism mitigates extracellular mitochondrial DNA accumulation in lung fibrosis models and in patients with idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Genta Ishikawa,Xueyan Peng,John McGovern,Sam Woo,Carrighan Perry,Angela Liu,Sheeline Yu,Alexander Ghincea,Aliaksandr Kishchanka,Vitória Fiorini,Buqu Hu,Ying Sun,Huanxing Sun,Changwan Ryu,Erica L Herzog

Molecular therapy : the journal of the American Society of Gene Therapy 31:1615-1635 PubMed36566349

2022

A positive feedback circuit between RN7SK snRNA and mA readers is essential for tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xu,Lifang Ma,Xiao Zhang,Susu Guo,Wanxin Guo,Yikun Wang,Shiyu Qiu,Xiaoting Tian,Yayou Miao,Yongchun Yu,Jiayi Wang

Clinical and translational medicine 12:e890 PubMed35758323

2022

Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqiang He,Qi Fu,Min Sun,Yu Qian,Yucheng Liang,Jie Zhang,Rui Gao,Hemin Jiang,Hao Dai,Yuwei Liu,Xinyu Xu,Heng Chen,Kuanfeng Xu,Tao Yang

Proceedings of the National Academy of Sciences of the United States of America 119:e2123511119 PubMed35537053

2022

α-adrenoceptors regulate chemokine receptor-mediated leukocyte migration via formation of heteromeric receptor complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Garrett A Enten,Xianlong Gao,Hannah R Strzelinski,McWayne Weche,Stephen B Liggett,Matthias Majetschak

PloS one 16:e0253821 PubMed34166476

2021

Plasticity of seven-transmembrane-helix receptor heteromers in human vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren J Albee,Xianlong Gao,Matthias Majetschak

FEBS letters 595:1863-1875 PubMed34032285

2021

Class A G protein-coupled receptors assemble into functional higher-order hetero-oligomers.

Applications

Unspecified application

Species

Unspecified reactive species

Xianlong Gao,Garrett A Enten,Anthony J DeSantis,Matthias Majetschak

Biochemical pharmacology 188:114560 PubMed33844984

2021

GPR55 regulates the responsiveness to, but does not dimerise with, α-adrenoceptors.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah K Walsh,Christopher Lipina,Sheng Y Ang,Masaaki Sato,Ling Yeong Chia,Martina Kocan,Dana S Hutchinson,Roger J Summers,Cherry L Wainwright

Gerontology 67:323-337 PubMed33752204

2021

Mechanism of α1-Adrenergic Receptor-Induced Increased Contraction of Rat Mesenteric Artery in Aging Hypertension Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Wei,Ting Lan,Yuanqun Zhou,Jun Cheng,Pengyun Li,Xiaorong Zeng,Yan Yang
View all publications
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Complementary Products

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Secondary Antibodies

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Product promise

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