AB166925

Anti-alpha 1b Adrenergic Receptor antibody

Name: Anti-alpha 1b Adrenergic Receptor antibody (ab166925) | Abcam
Brand: Abcam Limited
SKU: AB166925
Price: 535 USD
Availability: InStock

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(4 Publications)

Goat Polyclonal ADRA1B antibody. Suitable for WB and reacts with Mouse, Rat, Pig, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ADRA1B aa 250-300.

View Alternative Names

Alpha-1B adrenergic receptor, Alpha-1B adrenoreceptor, Alpha-1B adrenoceptor, ADRA1B

2 Images

Supplier Data

Western blot - Anti-alpha 1b Adrenergic Receptor antibody (AB166925)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-alpha 1b Adrenergic Receptor antibody (ab166925) at 0.1 µg/mL

Lane 1:

Human heart lysate (in RIPA buffer) at 35 µg

Lane 2:

Mouse heart lysate (in RIPA buffer) at 35 µg

Lane 3:

Rat heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 60 kDa

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Supplier Data

Western blot - Anti-alpha 1b Adrenergic Receptor antibody (AB166925)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-alpha 1b Adrenergic Receptor antibody (ab166925) at 0.1 µg/mL

All lanes:

Pig heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 60 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

applications

Immunogen

Synthetic Peptide within Human ADRA1B aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P35368

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab166925 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The alpha 1d adrenergic receptor also known as the alpha-1d adrenoceptor is a G-protein-coupled receptor with mass approximately 70 kDa. It plays a critical role in the modulation of physiological responses by binding catecholamines like norepinephrine and epinephrine. Alpha-1d adrenergic receptor is widely expressed in vascular smooth muscle heart liver and central nervous system. This receptor remains an important target for pharmacological research due to its involvement in various physiological processes.

Biological function summary

The alpha-1d adrenergic receptor promotes vasoconstriction and increases blood pressure through the activation of phospholipase C pathway resulting in calcium release from intracellular stores. It functions as part of a receptor complex that interacts with other alpha-1 adrenergic subtypes such as alpha-1a and alpha-1b adrenergic receptors differentiating their roles in tissue-specific actions. These receptor activities affect muscle contraction and influence systemic vascular resistance.

Pathways

This adrenergic receptor is engaged in the adrenergic signaling in cardiomyocytes and vascular smooth muscle contraction pathways. It facilitates communication between the signaling molecules and cellular responses. The alpha-1d adrenergic receptor partners with proteins like Gq/11 protein family which mediates signal transduction leading to its physiological effects. Interaction with other receptors such as beta-adrenergic receptors shapes the overall cardiovascular outcomes by providing a balance between constriction and relaxation.

The alpha-1d adrenergic receptor links to hypertension and benign prostatic hyperplasia where its modulation can influence disease progression and treatment outcomes. In hypertension dysregulation of alpha-1d adrenergic signaling may alter vascular tone and resistance contributing to elevated blood pressure. Connections with proteins such as phospholipase C confirm its involvement in the signaling cascade that affects these conditions. Modulation of this receptor activity offers therapeutic potential for related cardiovascular and urological disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Alpha-1 adrenergic receptors are G protein-coupled receptors for catecholamines that signal through the G(q) family of G proteins, including G(q) and G(11). Upon activation, they stimulate the phosphatidylinositol-calcium second messenger pathway, leading to calcium release from intracellular stores and activation of protein kinase C (By similarity). ADRA1B binds the catecholamine ligands norepinephrine and epinephrine (PubMed : 7815325, PubMed : 8183249). Can also couple to G(14) and G(16) proteins (By similarity). Nuclear ADRA1B forms heterooligomers with ADRA1A to regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes (PubMed : 18802028, PubMed : 22120526). At the plasma membrane, ADRA1B interacts with CAVIN4/MURC to regulates ERK activation in cardiomyocytes, contributing to the regulation of cardiac hypertrophy (PubMed : 24567387).

See full target information ADRA1B

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Actas espanolas de psiquiatria 52:83-98 PubMed38622006

2024

Bioinformatic Identification of Signaling Pathways and Hub Genes in Vascular Dementia.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanhua Wu,Jing Cai,Bo Pang,Liping Cao,Qiankun He,Qiansong He,Anbang Zhang

BMC neuroscience 25:5 PubMed38291397

2024

Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α-adrenergic receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoyong Tian,Yang Yang,Renfeng Wang,Yao Li,Fei Sun,Jun Chen,Dingjun Zha

International journal of molecular medicine 49: PubMed35014672

2022

Overexpression of miR‑375 reverses the effects of dexamethasone on the viability, migration, invasion and apoptosis of human airway epithelial cells by targeting DUSP6.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Zheng,Chunlian Li,Xiang Gao

Free radical biology & medicine 163:190-195 PubMed33352221

2020

Terazosin reduces steroidogenic factor 1 and upregulates heat shock protein 90 expression in LH-induced bovine ovarian theca cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Run Tang,Shou-Long Deng,Zheng-Xing Lian,Kun Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-alpha 1b Adrenergic Receptor antibody

Western blot - Anti-alpha 1b Adrenergic Receptor antibody (AB166925)

Western blot - Anti-alpha 1b Adrenergic Receptor antibody (AB166925)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise