AB84402

Anti-alpha 1d Adrenergic Receptor antibody

Name: Anti-alpha 1d Adrenergic Receptor antibody (ab84402) | Abcam
Brand: Abcam Limited
SKU: AB84402
Price: 535 USD
Availability: InStock

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(8 Publications)

Rabbit Polyclonal alpha 1d Adrenergic Receptor antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human ADRA1D.

View Alternative Names

ADRA1A, ADRA1D, Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-adrenergic receptor 1a, Alpha-1D adrenoceptor

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1d Adrenergic Receptor antibody (AB84402)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1d Adrenergic Receptor antibody (AB84402)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical cancer tissue labelling alpha 1d Adrenergic Receptor with ab84402 at a dilution of 1/100.

Unknown

Western blot - Anti-alpha 1d Adrenergic Receptor antibody (AB84402)

All lanes:

Western blot - Anti-alpha 1d Adrenergic Receptor antibody (ab84402) at 1/1000 dilution

Lane 1:

A549 lysate

Lane 2:

MCF7 lysate

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ADRA1D. The exact immunogen used to generate this antibody is proprietary information.

P25100

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The alpha 1d adrenergic receptor also known as the alpha-1d adrenoceptor is a G-protein-coupled receptor with mass approximately 70 kDa. It plays a critical role in the modulation of physiological responses by binding catecholamines like norepinephrine and epinephrine. Alpha-1d adrenergic receptor is widely expressed in vascular smooth muscle heart liver and central nervous system. This receptor remains an important target for pharmacological research due to its involvement in various physiological processes.

Biological function summary

The alpha-1d adrenergic receptor promotes vasoconstriction and increases blood pressure through the activation of phospholipase C pathway resulting in calcium release from intracellular stores. It functions as part of a receptor complex that interacts with other alpha-1 adrenergic subtypes such as alpha-1a and alpha-1b adrenergic receptors differentiating their roles in tissue-specific actions. These receptor activities affect muscle contraction and influence systemic vascular resistance.

Pathways

This adrenergic receptor is engaged in the adrenergic signaling in cardiomyocytes and vascular smooth muscle contraction pathways. It facilitates communication between the signaling molecules and cellular responses. The alpha-1d adrenergic receptor partners with proteins like Gq/11 protein family which mediates signal transduction leading to its physiological effects. Interaction with other receptors such as beta-adrenergic receptors shapes the overall cardiovascular outcomes by providing a balance between constriction and relaxation.

The alpha-1d adrenergic receptor links to hypertension and benign prostatic hyperplasia where its modulation can influence disease progression and treatment outcomes. In hypertension dysregulation of alpha-1d adrenergic signaling may alter vascular tone and resistance contributing to elevated blood pressure. Connections with proteins such as phospholipase C confirm its involvement in the signaling cascade that affects these conditions. Modulation of this receptor activity offers therapeutic potential for related cardiovascular and urological disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Alpha-1 adrenergic receptors are G protein-coupled receptors for catecholamines that signal through the G(q) family of G proteins, including G(q) and G(11). Upon activation, they stimulate the phosphatidylinositol-calcium second messenger pathway, leading to calcium release from intracellular stores and activation of protein kinase C (PubMed : 7746284). ADRA1D binds the catecholamine ligands norepinephrine and epinephrine (PubMed : 7815325, PubMed : 8024574, PubMed : 8183249).

See full target information ADRA1D

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Lung cellular and molecular physiology 324:L639-L651 PubMed36648147

2023

α1 Adrenoreceptor antagonism mitigates extracellular mitochondrial DNA accumulation in lung fibrosis models and in patients with idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Genta Ishikawa,Xueyan Peng,John McGovern,Sam Woo,Carrighan Perry,Angela Liu,Sheeline Yu,Alexander Ghincea,Aliaksandr Kishchanka,Vitória Fiorini,Buqu Hu,Ying Sun,Huanxing Sun,Changwan Ryu,Erica L Herzog

Clinical and translational medicine 12:e890 PubMed35758323

2022

Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqiang He,Qi Fu,Min Sun,Yu Qian,Yucheng Liang,Jie Zhang,Rui Gao,Hemin Jiang,Hao Dai,Yuwei Liu,Xinyu Xu,Heng Chen,Kuanfeng Xu,Tao Yang

Proceedings of the National Academy of Sciences of the United States of America 119:e2123511119 PubMed35537053

2022

α-adrenoceptors regulate chemokine receptor-mediated leukocyte migration via formation of heteromeric receptor complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Garrett A Enten,Xianlong Gao,Hannah R Strzelinski,McWayne Weche,Stephen B Liggett,Matthias Majetschak

Investigative and clinical urology 61:297-303 PubMed32377606

2020

Expression of bladder α-adrenoceptor subtype after relief of partial bladder outlet obstruction in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Yong Lee,Jong Mok Park,Yong Gil Na,Ki Hak Song,Jae Sung Lim,Seung Woo Yang,Gun Hwa Kim,Ju Hyun Shin

Circulation research 123:1080-1090 PubMed30355157

2018

Response Gene to Complement 32 Maintains Blood Pressure Homeostasis by Regulating α-Adrenergic Receptor Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Ming Tang,Ning Shi,Kun Dong,Scott A Brown,Amanda E Coleman,Matthew A Boegehold,Shi-You Chen

Naunyn-Schmiedeberg's archives of pharmacology 389:243-8 PubMed26660071

2015

Commercially available antibodies directed against α-adrenergic receptor subtypes and other G protein-coupled receptors with acceptable selectivity in flow cytometry experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Tripathi,Vadim Gaponenko,Matthias Majetschak

Proceedings of the National Academy of Sciences of 112:E1659-68 PubMed25775528

2015

Heteromerization of chemokine (C-X-C motif) receptor 4 with α1A/B-adrenergic receptors controls α1-adrenergic receptor function.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Tripathi,P Geoff Vana,Tanmay S Chavan,Lioubov I Brueggemann,Kenneth L Byron,Nadya I Tarasova,Brian F Volkman,Vadim Gaponenko,Matthias Majetschak

Journal of cardiovascular pharmacology 57:263-6 PubMed21107280

2010

Selective enhancement of contractions to α1-adrenergic receptor activation in the aorta of mice with sickle cell disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ramiro Juncos,Luis Juncos,Robert P Hebbel,Gregory M Vercellotti,Zvonimir S Katusic,Karl A Nath

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-alpha 1d Adrenergic Receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1d Adrenergic Receptor antibody (AB84402)

Western blot - Anti-alpha 1d Adrenergic Receptor antibody (AB84402)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise