AB150414

Anti-Alpha 2 Antiplasmin antibody [EPSIS17]

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Alpha 2 Antiplasmin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

AAP, PLI, SERPINF2, Alpha-2-antiplasmin, Alpha-2-AP, Alpha-2-plasmin inhibitor, Serpin F2, Alpha-2-PI

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (AB150414)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (AB150414)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Alpha 2 Antiplasmin with ab150414 at 1/150 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Western blot - Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (AB150414)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (ab150414) at 1/10000 dilution

All lanes:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 65 kDa

false

Exposure time: 5s

Carrier free

Anti-Alpha 2 Antiplasmin antibody [EPSIS17] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPSIS17

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "Heat up to 98°C, below boiling, and then let cool for 10-20 min.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product has switched from a hybridoma to recombinant production method on 2nd August 2023.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 2 Antiplasmin also known as A2AP or anti-alpha 2 plays an important role in inhibiting the fibrinolytic system by inactivating plasmin. Weighing around 70 kDa this glycoprotein is mainly expressed in the liver and circulates in plasma. Its alternative names include alpha-2-antiplasmin and anti-alpha 2 which highlight its blocking role against excess plasmin action.

Biological function summary

Alpha 2 Antiplasmin functions as the primary inhibitor of plasmin blocking unwanted fibrinolysis. It can form a complex with fibrin which enhances its efficiency in preventing premature fibrin clot dissolution. This regulation of plasmin activity ensures the stability of clots essential for proper wound healing and controlling bleeding.

Pathways

Alpha 2 Antiplasmin engages significantly with the fibrinolysis and coagulation pathways. Its action balances the fibrinolytic process which involves transforming plasminogen to plasmin. Related proteins like plasminogen activator inhibitor also regulate plasmin maintaining equilibrium between clot formation and degradation.

Alpha 2 Antiplasmin has implications in hemorrhagic conditions and thrombotic disorders. Its deficiency might lead to excessive bleeding due to unregulated plasmin activity whilst elevated levels could contribute to thrombotic problems causing harmful clot formation. The interplay with plasminogen and its activation is important in understanding its disease linkages.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.

See full target information SERPINF2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 31:1219-1234 PubMed38867004

2024

The tRNA Gm18 methyltransferase TARBP1 promotes hepatocellular carcinoma progression via metabolic reprogramming of glutamine.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Shi,Yangyi Zhang,Yuci Wang,Jie Wang,Yang Gao,Ruiqi Wang,Liyong Wang,Minggang Xiong,Yanlan Cao,Ningjing Ou,Qi Liu,Honghui Ma,Jiabin Cai,Hao Chen

Proceedings of the National Academy of Sciences of 115:E9687-E9696 PubMed30254165

2018

Blood-derived plasminogen drives brain inflammation and plaque deposition in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah K Baker,Zu-Lin Chen,Erin H Norris,Alexey S Revenko,A Robert MacLeod,Sidney Strickland

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha 2 Antiplasmin antibody [EPSIS17]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (AB150414)

Western blot - Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (AB150414)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise