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Rabbit Recombinant Monoclonal alpha 2 Macroglobulin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-73] - BSA and Azide free (Capture) (AB323295), expandable thumbnail
  • Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-73] - BSA and Azide free (Capture) (AB323295), expandable thumbnail
  • Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-73] - BSA and Azide free (Capture) (AB323295), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Alternative names

Rabbit Recombinant Monoclonal alpha 2 Macroglobulin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR24425-73

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab323295 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human alpha 2 Macroglobulin. The recommended pair for sandwich ELISA is:

Capture: ab323295, Human alpha 2 Macroglobulin Capture Antibody (unconjugated)

Detector: Anti-alpha 2 Macroglobulin antibody [EPR24425-139] - BSA and Azide free (Detector) ab323296, Human alpha 2 Macroglobulin Detector Antibody (unconjugated)

The reference range value is 0.08-20 ng/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-73] - BSA and Azide free (Capture) (ab323295), expandable thumbnail

    Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-73] - BSA and Azide free (Capture) (ab323295)

    Interpolated concentration of native alpha 2 Macroglobulin was measured at different sample concentrations. Undiluted samples are as follows: human urine 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-73] - BSA and Azide free (Capture) (ab323295), expandable thumbnail

    Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-73] - BSA and Azide free (Capture) (ab323295)

    Sandwich ELISA of Human alpha 2 Macroglobulin Antibody Pair - BSA and Azide free ab323294 with the capture antibody ab323295 dilution at 2 ug/mL and detector antibody Anti-alpha 2 Macroglobulin antibody [EPR24425-139] - BSA and Azide free (Detector) ab323296 dilution at 0.5 ug/mL.

  • Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-73] - BSA and Azide free (Capture) (ab323295), expandable thumbnail

    Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-73] - BSA and Azide free (Capture) (ab323295)

    Interpolated concentration of native alpha 2 Macroglobulin was measured at different sample concentrations. Undiluted samples are as follows: human plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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