JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB156015

Anti-alpha 2 Macroglobulin antibody [EPSISR39]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal alpha 2 Macroglobulin antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CPAMD5, FWP007, A2M, Alpha-2-macroglobulin, Alpha-2-M, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-alpha 2 Macroglobulin antibody [EPSISR39] (AB156015)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-alpha 2 Macroglobulin antibody [EPSISR39] (AB156015)

Immunofluorescent analysis of HepG2 cells labeling alpha 2 Macroglobulin with ab156015 at 1/100 dilution.

Western blot - Anti-alpha 2 Macroglobulin antibody [EPSISR39] (AB156015)
  • WB

Unknown

Western blot - Anti-alpha 2 Macroglobulin antibody [EPSISR39] (AB156015)

All lanes:

Western blot - Anti-alpha 2 Macroglobulin antibody [EPSISR39] (ab156015) at 1/10000 dilution

Lane 1:

Human serum lysate at 10 µg

Lane 2:

Human plasma lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 163 kDa

false

  • Carrier free

    Anti-alpha 2 Macroglobulin antibody [EPSISR39] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPSISR39

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB7338

Biotin Anti-alpha 2 Macroglobulin antibody

  • 0
  • 0
Primary Antibodies

AB181656

Anti-alpha 2 Macroglobulin antibody

Western blot - Anti-alpha 2 Macroglobulin antibody (AB181656)

  • 0
  • 0
Primary Antibodies

AB109422

Anti-alpha 2 Macroglobulin antibody [EPR4432]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2 Macroglobulin antibody [EPR4432] (AB109422)

  • 0
  • 0
Primary Antibodies

AB133299

Anti-alpha 2 Macroglobulin antibody [EPR4431]

RabMAb|Recombinant

Western blot - Anti-alpha 2 Macroglobulin antibody [EPR4431] (AB133299)

  • 0
  • 0

Complementary Products

Assay Kits

AB322431

Human alpha 2 Macroglobulin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB322431)

  • 0
  • 0
Primary Antibodies

AB32136

Anti-Amyloid Precursor Protein antibody [Y188]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [Y188] (AB32136)

  • 4
  • 9
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
Primary Antibodies

AB259716

Anti-Alpha 2 Antiplasmin antibody [EPR22550-263] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Alpha 2 Antiplasmin antibody [EPR22550-263] - BSA and Azide free (Detector) (AB259716)

  • 0
  • 0
Primary Antibodies

AB109503

Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control

20ul selling size|RabMAb|Recombinant|Lab Essentials|Trial Size

Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (AB109503)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-2-macroglobulin also known as alpha-2-M and A2M is a large glycoprotein with a molecular mass of approximately 725 kDa. It is expressed in the liver and is found in high concentrations in the blood plasma. This protein functions mechanically as a broad-spectrum protease inhibitor. It can trap proteases within its structure preventing them from accessing substrates. Additionally alpha-2-macroglobulin can bind to cytokines and growth factors altering their availability and activities.
Biological function summary

Alpha-2-macroglobulin plays an important role in maintaining homeostasis by regulating protease activities and modulating immune system reactions. It does not form a permanent complex but can transiently associate with the proteases and various molecules mentioned. By interacting with proteases it controls processes like coagulation and fibrinolysis and participates in the clearance of proteinases and growth factors from the circulation. It influences cell signaling pathways and inflammatory responses highlighting its significance in maintaining balance within the body.

Pathways

Alpha-2-macroglobulin is an important component in the immune response and coagulation pathways. In the complement system it binds with complement components to regulate their activities. It associates with proteins such as plasmin and thrombin which are important for blood clotting and fibrinolysis. These relationships emphasize the protein's involvement in both protective mechanisms against pathogens and the regulation of blood coagulation and breakdown connecting the immune and hemostatic systems.

Alpha-2-macroglobulin has been linked to Alzheimer's disease and liver diseases. In Alzheimer's altered levels of this protein might affect the amyloid-beta peptide clearance due to its protease inhibition and binding properties. It associates with proteins like lipoprotein receptor-related protein-1 (LRP1) influencing amyloid-beta metabolism. In liver diseases its levels can indicate liver dysfunction or inflammation given its synthesis in the liver and can relate to proteins involved in inflammation such as cytokines. Its modulation of protease activity makes it a critical target in understanding these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
See full target information A2M
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:601-619 PubMed39839187

2025

MAP17 is a Novel NASH Progression Biomarker Associated with Macrophage Infiltration, Immunotherapy Response, and Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Huang,Jiatong Chen,Shenglu Liu,Xin Xiang,Yang Long,Peng Tan,Wenguang Fu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com