AB181866

Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal alpha A Crystallin/CRYAA antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 6 publications.

View Alternative Names

CRYA1, HSPB4, CRYAA, Alpha-crystallin A chain, Heat shock protein beta-4, Heat shock protein family B member 4, HspB4

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (AB181866)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (AB181866)

Immunofluorescence analysis of Y79 cells fixed with acetone labeling alpha A Crystallin/CRYAA with ab181866 at 1/500 dilution followed by Goat anti rabbit IgG(Alexa Fluor®555) at 1/200 dilution and counterstained with Dapi.

Supplier Data

Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (AB181866)

All lanes:

Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866) at 1/2000 dilution

Lane 1:

Rat eyeball at 20 µg

Lane 2:

Mouse eyeball at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

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Carrier free

Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14125(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha A Crystallin also known as CRYAA or crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is the major structural protein in the eye lens and is also expressed in other tissues like the heart kidney and brain. The protein forms large complexes with other alpha crystallin proteins to provide structural stability and help maintain the transparency and refractive index of the lens. This specific role of alpha A Crystallin makes it essential for proper eye function.

Biological function summary

Alpha A Crystallin acts like a molecular chaperone. It helps prevent the aggregation of misfolded proteins which is important in maintaining cellular protein homeostasis. Alpha A Crystallin can combine with alpha B Crystallin in lens fiber cells to form a hetero-oligomeric complex. This ability to bind unfolded proteins and assist in refolding processes highlights its importance in cell stress responses especially in situations where proteins could potentially denature and lose function.

Pathways

Alpha A Crystallin participates significantly in the oxidative stress response and apoptotic pathways. It interacts with proteins involved in these processes such as caspases to inhibit cell death and curb the deleterious effects of reactive oxygen species. In the apoptotic pathway alpha A Crystallin's role in regulating cell survival highlights its association with proteins like Hsp70 another heat shock protein that serves as a stabilizing agent during cellular stress.

Certain mutations in alpha A Crystallin link it to cataracts and age-related macular degeneration. These ocular conditions arise when the protein's chaperone function declines leading to the aggregation of lens proteins and subsequent loss of lens transparency. Alpha B Crystallin the sister protein shows a similar role in these disorders indicating how disturbances in the intricate network of crystallin interactions affect ocular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Contributes to the transparency and refractive index of the lens (PubMed : 18302245). In its oxidized form (absence of intramolecular disulfide bond), acts as a chaperone, preventing aggregation of various proteins under a wide range of stress conditions (PubMed : 18199971, PubMed : 19595763, PubMed : 22120592, PubMed : 31792453). Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA (PubMed : 28935373).

See full target information CRYAA

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:575 PubMed40830790

2025

Engineered MEVs for photoreceptor-targeted delivery of USP25 to alleviate diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoxiang Sun,Shenyuan Chen,Xiaoyuan Qi,Yuntong Sun,Zhengmei Jiang,Jie Chang,Yongjun Ma,Jin Huang,Benshuai You,Fengtian Sun

Frontiers in cell and developmental biology 12:1526943 PubMed39834393

2025

Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Yufan Liu,Zuowei Wang,Tianju Ma,Yi Gao,Wenqian Chen,Zi Ye,Zhaohui Li

Heliyon 10:e23690 PubMed38187316

2024

The E156K mutation in the gene affects the epithelial-mesenchymal transition and migration of human lens epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhennan Zhao,Jiahui Chen,Yongxiang Jiang,Yi Lu

Molecular vision 28:317-330 PubMed36338667

2022

Structural and functional analysis of SNP rs76740365 G>A in exon-3 of the alpha A-crystallin gene in lens epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhennan Zhao,Yang Sun,Qi Fan,Yongxiang Jiang,Yi Lu

Ophthalmic research 63:564-571 PubMed32305986

2020

Up-Regulation of GATA4 Regulates Human Lens Epithelial Cell Function in Age-Related Cataract.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Xie,Xiaofei Song,Xin Liu,Xiaogang Luo,Maidina Nabijiang,Mengqi Ma,Ying Li

Cellular physiology and biochemistry : internation 50:246-260 PubMed30286462

2018

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Liu,Chang Liu,Kun Shan,Shujie Zhang,Yi Lu,Biao Yan,Yi Luo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)]

Immunocytochemistry/ Immunofluorescence - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (AB181866)

Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (AB181866)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise