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AB250535

Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal alpha A Crystallin/CRYAA antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

CRYA1, HSPB4, CRYAA, Alpha-crystallin A chain, Heat shock protein beta-4, Heat shock protein family B member 4, HspB4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (AB250535)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (AB250535)

This data was developed using ab181866, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of Y79 cells fixed with acetone labeling alpha A Crystallin/CRYAA with ab181866 at 1/500 dilution followed by Goat anti rabbit IgG(Alexa Fluor®555) at 1/200 dilution and counterstained with Dapi.

Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (AB250535)
  • WB

Supplier Data

Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (AB250535)

This data was developed using ab181866, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (<a href='/en-us/products/primary-antibodies/alpha-a-crystallin-cryaa-antibody-epr14125b-ab181866'>ab181866</a>) at 1/2000 dilution

Lane 1:

Rat eyeball at 20 µg

Lane 2:

Mouse eyeball at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false

  • Unconjugated

    Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14125(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250535 is the carrier-free version of ab181866.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha A Crystallin also known as CRYAA or crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is the major structural protein in the eye lens and is also expressed in other tissues like the heart kidney and brain. The protein forms large complexes with other alpha crystallin proteins to provide structural stability and help maintain the transparency and refractive index of the lens. This specific role of alpha A Crystallin makes it essential for proper eye function.
Biological function summary

Alpha A Crystallin acts like a molecular chaperone. It helps prevent the aggregation of misfolded proteins which is important in maintaining cellular protein homeostasis. Alpha A Crystallin can combine with alpha B Crystallin in lens fiber cells to form a hetero-oligomeric complex. This ability to bind unfolded proteins and assist in refolding processes highlights its importance in cell stress responses especially in situations where proteins could potentially denature and lose function.

Pathways

Alpha A Crystallin participates significantly in the oxidative stress response and apoptotic pathways. It interacts with proteins involved in these processes such as caspases to inhibit cell death and curb the deleterious effects of reactive oxygen species. In the apoptotic pathway alpha A Crystallin's role in regulating cell survival highlights its association with proteins like Hsp70 another heat shock protein that serves as a stabilizing agent during cellular stress.

Certain mutations in alpha A Crystallin link it to cataracts and age-related macular degeneration. These ocular conditions arise when the protein's chaperone function declines leading to the aggregation of lens proteins and subsequent loss of lens transparency. Alpha B Crystallin the sister protein shows a similar role in these disorders indicating how disturbances in the intricate network of crystallin interactions affect ocular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Contributes to the transparency and refractive index of the lens (PubMed : 18302245). In its oxidized form (absence of intramolecular disulfide bond), acts as a chaperone, preventing aggregation of various proteins under a wide range of stress conditions (PubMed : 18199971, PubMed : 19595763, PubMed : 22120592, PubMed : 31792453). Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA (PubMed : 28935373).
See full target information CRYAA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15564 PubMed38971897

2024

LILRB4 knockdown inhibits aortic dissection development by regulating pyroptosis and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxian Xiong,Jiayuan Ling,Jie Yan,Yanyu Duan,Junjian Yu,Wentong Li,Wenbo Yu,Jianfeng Gao,Dilin Xie,Ziyou Liu,Yongzhi Deng,Yongling Liao
View all publications
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Product promise

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