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Rabbit Recombinant Monoclonal alpha Actinin 4 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-alpha Actinin 4 antibody [EPR4410] - Loading Control (AB108201), expandable thumbnail
  • Western blot - Anti-alpha Actinin 4 antibody [EPR4410] - Loading Control (AB108201), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody [EPR4410] - Loading Control (AB108201), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Expected
Not recommended
Not recommended
Tested
Rat
Expected
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778). Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (PubMed:22689882).

Alternative names

Alpha-actinin-4, Non-muscle alpha-actinin 4, ACTN4

Recommended products

Rabbit Recombinant Monoclonal alpha Actinin 4 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4410
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha Actinin 4 sometimes referred to as ACTN4 or alpha-actinin is a protein with a mass of approximately 100 kDa. It functions as an actin-binding protein particularly involved in the crosslinking of actin filaments. This protein is expressed widely with high levels found in muscle kidney and brain tissues. Alpha-actinin 4 also plays important roles in cellular structure and integrity by binding actin filaments in various cellular contexts.

Biological function summary

The role of alpha-actinin 4 extends to maintaining cytoskeletal structure and cell motility. It forms part of the larger actin-binding complex that supports cellular adhesion and mechanical stability. By linking actin filaments it allows cells to withstand mechanical stress and maintain their shape. Additionally alpha-actinin 4 contributes to cellular processes like cytokinesis and signal transduction by serving as a scaffold for signaling proteins.

Pathways

Alpha-actinin 4 interacts with diverse cellular signaling mechanisms. Key pathways include the actin cytoskeleton signaling and integrin-mediated cell adhesion. In these pathways alpha-actinin 4 works alongside proteins such as integrins and vinculin facilitating communication between the extracellular matrix and the cytoskeleton. These interactions are critical for cellular responses to environmental stimuli and adaptation during movement or development.

Associated diseases and disorders

Alpha-actinin 4 has notable impacts on conditions like focal segmental glomerulosclerosis (FSGS) and cancer. In FSGS mutation or dysregulation of alpha-actinin 4 contributes to podocyte dysfunction leading to proteinuria and kidney damage. Its role in cancer often involves altered expression influencing cell migration and invasion. The interaction with other proteins like nephrin in FSGS or E-cadherin in cancer highlights the significance of alpha-actinin 4 in pathogenic processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-alpha Actinin 4 antibody [EPR4410] - Loading Control (ab108201), expandable thumbnail

    Western blot - Anti-alpha Actinin 4 antibody [EPR4410] - Loading Control (ab108201)

    Alpha Actinin 4 Western blot staining using rabbit Anti-alpha Actinin 4 antibody

    Lanes 1 - 3: Merged signal (red and green). Green - ab108201 observed at 105 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab108201 was shown to recognize alpha Actinin 4 in wild-type HAP1 cells as signal was lost at the expected MW in ACTN4 (alpha Actinin 4) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ACTN4 (alpha Actinin 4) knockout samples were subjected to SDS-PAGE. ab108201 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-alpha Actinin 4 antibody [EPR4410] - Loading Control (ab108201) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 40 µg

    Lane 2: ACTN4 (alpha Actinin 4) knockout HAP1 whole cell lysate at 40 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Predicted band size: 105 kDa

    Observed band size: 105 kDa

  • Western blot - Anti-alpha Actinin 4 antibody [EPR4410] - Loading Control (ab108201), expandable thumbnail

    Western blot - Anti-alpha Actinin 4 antibody [EPR4410] - Loading Control (ab108201)

    Alpha Actinin 4 Western blot staining using rabbit Anti-alpha Actinin 4 antibody

    All lanes: Western blot - Anti-alpha Actinin 4 antibody [EPR4410] - Loading Control (ab108201) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: MCF-7 cell lysate at 10 µg

    Lane 3: C2C12 cell lysate at 10 µg

    Lane 4: A673 cell lysate at 10 µg

    Lane 5: L6 cell lysate at 10 µg

    Predicted band size: 105 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody [EPR4410] - Loading Control (ab108201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody [EPR4410] - Loading Control (ab108201)

    Alpha Actinin 4 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-alpha Actinin 4 antibody

    ab108201, at 1/100 dilution, staining alpha Actinin 4 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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