AB222767

Anti-alpha Actinin 4 antibody [RM287] - Loading Control

Recombinant
Lab Essentials
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(1 Publication)

Rabbit Recombinant Monoclonal alpha Actinin 4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ACTN4.

View Alternative Names

Alpha-actinin-4, Non-muscle alpha-actinin 4, ACTN4

2 Images

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody [RM287] - Loading Control (AB222767)

Paraffin embedded human breast cancer tissue stained for alpha Actinin 4 with ab222767 at a 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-alpha Actinin 4 antibody [RM287] - Loading Control (AB222767)

All lanes:

Western blot - Anti-alpha Actinin 4 antibody [RM287] - Loading Control (ab222767) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 105 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM287

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ACTN4. The exact immunogen used to generate this antibody is proprietary information.

O43707

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Actinin 4 sometimes referred to as ACTN4 or alpha-actinin is a protein with a mass of approximately 100 kDa. It functions as an actin-binding protein particularly involved in the crosslinking of actin filaments. This protein is expressed widely with high levels found in muscle kidney and brain tissues. Alpha-actinin 4 also plays important roles in cellular structure and integrity by binding actin filaments in various cellular contexts.

Biological function summary

The role of alpha-actinin 4 extends to maintaining cytoskeletal structure and cell motility. It forms part of the larger actin-binding complex that supports cellular adhesion and mechanical stability. By linking actin filaments it allows cells to withstand mechanical stress and maintain their shape. Additionally alpha-actinin 4 contributes to cellular processes like cytokinesis and signal transduction by serving as a scaffold for signaling proteins.

Pathways

Alpha-actinin 4 interacts with diverse cellular signaling mechanisms. Key pathways include the actin cytoskeleton signaling and integrin-mediated cell adhesion. In these pathways alpha-actinin 4 works alongside proteins such as integrins and vinculin facilitating communication between the extracellular matrix and the cytoskeleton. These interactions are critical for cellular responses to environmental stimuli and adaptation during movement or development.

Alpha-actinin 4 has notable impacts on conditions like focal segmental glomerulosclerosis (FSGS) and cancer. In FSGS mutation or dysregulation of alpha-actinin 4 contributes to podocyte dysfunction leading to proteinuria and kidney damage. Its role in cancer often involves altered expression influencing cell migration and invasion. The interaction with other proteins like nephrin in FSGS or E-cadherin in cancer highlights the significance of alpha-actinin 4 in pathogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed : 15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed : 22351778). Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (PubMed : 22689882).

See full target information ACTN4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 14: PubMed39334865

2024

Biochemical Characterisation of the Short Isoform of Histone N-Terminal Acetyltransferase NAA40.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel Klavaris,Maria Kouma,Cem Ozdemir,Vicky Nicolaidou,Kyle M Miller,Costas Koufaris,Antonis Kirmizis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-alpha Actinin 4 antibody [RM287] - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody [RM287] - Loading Control (AB222767)

Western blot - Anti-alpha Actinin 4 antibody [RM287] - Loading Control (AB222767)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise