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Mouse Monoclonal alpha Actinin/ACTN1 antibody. Suitable for ICC and reacts with Human samples. Cited in 40 publications. Immunogen corresponding to Full Length Protein corresponding to Human ACTN1.


Images

Immunocytochemistry - Anti-alpha Actinin/ACTN1 antibody [AT6/172] (AB18061), expandable thumbnail
  • Immunocytochemistry - Anti-alpha Actinin/ACTN1 antibody [AT6/172] - Cytoskeleton Marker (AB18061), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.6
Preservative: 0.09% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Full Length Protein corresponding to Human ACTN1. The exact immunogen used to generate this antibody is proprietary information. Database link P12814

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIP
Human
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1-5 µg/mL
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

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Target data

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (PubMed:22689882).

Alternative names

Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1

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Mouse Monoclonal alpha Actinin/ACTN1 antibody. Suitable for ICC and reacts with Human samples. Cited in 40 publications. Immunogen corresponding to Full Length Protein corresponding to Human ACTN1.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Full Length Protein corresponding to Human ACTN1. The exact immunogen used to generate this antibody is proprietary information. Database link P12814
Clone number
AT6/172
Purification technique
Affinity purification Protein A
Specificity

Recognizes and is specific for human alpha Actinin 1/ACTN1 isoform (non-muscle) at 100 kDa.

This antibody may recognize other isoforms of alpha-actinin (1-4). There is no specific epitope information available.

The clone number has been updated from (0.T.02) to (AT6/172) both clone numbers name the same antibody clone.

Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha Actinin-1 also known as ACTN1 or Actn 1 is a cytoskeletal protein that cross-links actin filaments in muscle and non-muscle cells. With a mass of approximately 100 kDa it is an important component of cell structures such as Z-discs in skeletal muscle. Alpha Actinin is expressed in a range of tissues including skeletal cardiac and smooth muscle as well as in various non-muscle cells. Its main role is to organize the actin cytoskeleton providing structural support and enabling cell motility.

Biological function summary

Alpha Actinin-1 helps maintain the structural integrity of cells by anchoring actin filaments and connecting them to integrins or membrane proteins. It often functions as part of a protein complex interacting with other cytoskeletal components and signaling molecules to stabilize cellular structures. In muscle cells it blends with titin and other sarcomeric proteins to assist muscle contraction. Beyond structural roles it partakes in cellular signaling pathways by interacting with signaling proteins and influencing cellular responses to external stimuli.

Pathways

Alpha Actinin-1 is involved in the regulation of the actin cytoskeleton pathway as well as the integrin signaling pathway. In these pathways it collaborates with proteins such as vinculin and paxillin modulating cell adhesion and movement. Its ability to interact with integrin-linked kinases suggests a role in transducing mechanical signals from the extracellular matrix to downstream signaling cascades.

Associated diseases and disorders

Alpha Actinin-1 has been linked to a spectrum of muscular diseases and certain cancers. Mutations in Alpha Actinin-1 can contribute to hereditary diseases like congenital kidney disorders which affect actin filament organization in podocytes. In oncology altered expression levels of Alpha Actinin-1 can affect cell proliferation impacting tumor growth and metastasis. Its interaction with other proteins like focal adhesion kinase (FAK) underlines its involvement in these pathological conditions influencing cellular adhesion and signal transduction.

Product promise

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2 product images

  • Immunocytochemistry - Anti-alpha Actinin/ACTN1 antibody [AT6/172] (ab18061), expandable thumbnail

    Immunocytochemistry - Anti-alpha Actinin/ACTN1 antibody [AT6/172] (ab18061)

    Immunofluorescence staining of Actinin/ACTN1 using ab18061 in ioSkeletal Myocytes - Human iPSC derived cells ab277612, which were differentiated for 10 days post induction.

    The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab18061 at 5 µg/mL and Anti-beta Tubulin antibody - Loading Control ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).


    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.

  • Immunocytochemistry - Anti-alpha Actinin/ACTN1 antibody [AT6/172] - Cytoskeleton Marker (ab18061), expandable thumbnail

    Immunocytochemistry - Anti-alpha Actinin/ACTN1 antibody [AT6/172] - Cytoskeleton Marker (ab18061)

    Alpha Actinin/ACTN1 Immunocytochemistry staining using mouse Anti-alpha Actinin/ACTN1 antibody

    ICC/IF image of ab18061 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3 M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18061, 1 μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.

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