Skip to main content

Rabbit Recombinant Monoclonal alpha Actinin/ACTN1 antibody. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 14 publications.


Images

Immunoprecipitation - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (AB68194), expandable thumbnail
  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (AB68194), expandable thumbnail
  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (AB68194), expandable thumbnail
  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (AB68194), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] (AB68194), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Tested
Expected
Tested
Not recommended
Mouse
Expected
Tested
Tested
Not recommended
Rat
Expected
Expected
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
0.5-5 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/20
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (PubMed:22689882).

Alternative names

Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1

Recommended products

Rabbit Recombinant Monoclonal alpha Actinin/ACTN1 antibody. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP2527Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha Actinin-1 also known as ACTN1 or Actn 1 is a cytoskeletal protein that cross-links actin filaments in muscle and non-muscle cells. With a mass of approximately 100 kDa it is an important component of cell structures such as Z-discs in skeletal muscle. Alpha Actinin is expressed in a range of tissues including skeletal cardiac and smooth muscle as well as in various non-muscle cells. Its main role is to organize the actin cytoskeleton providing structural support and enabling cell motility.

Biological function summary

Alpha Actinin-1 helps maintain the structural integrity of cells by anchoring actin filaments and connecting them to integrins or membrane proteins. It often functions as part of a protein complex interacting with other cytoskeletal components and signaling molecules to stabilize cellular structures. In muscle cells it blends with titin and other sarcomeric proteins to assist muscle contraction. Beyond structural roles it partakes in cellular signaling pathways by interacting with signaling proteins and influencing cellular responses to external stimuli.

Pathways

Alpha Actinin-1 is involved in the regulation of the actin cytoskeleton pathway as well as the integrin signaling pathway. In these pathways it collaborates with proteins such as vinculin and paxillin modulating cell adhesion and movement. Its ability to interact with integrin-linked kinases suggests a role in transducing mechanical signals from the extracellular matrix to downstream signaling cascades.

Associated diseases and disorders

Alpha Actinin-1 has been linked to a spectrum of muscular diseases and certain cancers. Mutations in Alpha Actinin-1 can contribute to hereditary diseases like congenital kidney disorders which affect actin filament organization in podocytes. In oncology altered expression levels of Alpha Actinin-1 can affect cell proliferation impacting tumor growth and metastasis. Its interaction with other proteins like focal adhesion kinase (FAK) underlines its involvement in these pathological conditions influencing cellular adhesion and signal transduction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194), expandable thumbnail

    Immunoprecipitation - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194)

    Purified ab68194 at 1:20 dilution (2μg) immunoprecipitating alpha Actinin/ACTN1 in NIH/3T3 whole cell lysate.
    Lane 1 (input): NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10μg
    Lane 2 (+): ab68194 + NIH/3T3 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab68194 in NIH/3T3 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) (1:5000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 103 kDa

    All lanes: Immunoprecipitation - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194)

    Predicted band size: 103 kDa

  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194), expandable thumbnail

    Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194)

    Alpha Actinin/ACTN1 Western blot staining of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate using rabbit Anti-alpha Actinin/ACTN1 antibody

    Blocking buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194) at 1/10000 dilution

    All lanes: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 103 kDa

    Observed band size: 103 kDa

  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194), expandable thumbnail

    Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194)

    Alpha Actinin/ACTN1 Western blot staining using rabbit Anti-alpha Actinin/ACTN1 antibody

    Blocking buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194) at 1/1000 dilution

    Lane 1: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Lane 2: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 103 kDa

    Observed band size: 103 kDa

  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194), expandable thumbnail

    Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194)

    Alpha Actinin/ACTN1 Western blot staining using rabbit Anti-alpha Actinin/ACTN1 antibody

    Lanes 1- 2: Merged signal (red and green). Green - ab68194 observed at 103 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab68194 was shown to react with alpha Actinin/ACTN1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human ACTN1 (alpha Actinin) knockout HeLa cell line ab265610 (knockout cell lysate Human ACTN1 (alpha Actinin) knockout HeLa cell lysate ab257337) was used. Wild-type HeLa and ACTN1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab68194 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194) at 1/2000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ACTN1 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human ACTN1 (alpha Actinin) knockout HeLa cell line (Human ACTN1 (alpha Actinin) knockout HeLa cell line ab265610)

    Performed under reducing conditions.

    Predicted band size: 103 kDa

    Observed band size: 103 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] (ab68194), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] (ab68194)

    Immunofluorescence staining of Actinin/ACTN1 using ab68194 in ioSkeletal Myocytes - Human iPSC derived cells ab277612, which were differentiated for 3 (left panel), 5 (middle panel) and 10 days (right panel) post induction.

    The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab68194 at 0.5 µg/mL and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).


    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] (ab68194), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] (ab68194)

    Immunofluorescence staining of Actinin/ACTN1 using ab68194 in ioSkeletal Myocytes - Human iPSC derived cells ab277612, which were differentiated for 3 (left panel), 5 (middle panel) and 10 days (right panel) post induction.

    The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab68194 at 0.5 µg/mL and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).


    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.

  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194), expandable thumbnail

    Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194)

    Alpha Actinin/ACTN1 Western blot staining using rabbit Anti-alpha Actinin/ACTN1 antibody

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: Alpha Actinin/ACTN1 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: NIH 3T3 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab68194 observed at 103 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab68194 was shown to specifically react with alpha Actinin/ACTN1 in wild-type HAP1 cells as signal was lost in alpha Actinin/ACTN1 knockout cells. Wild-type and alpha Actinin/ACTN1 knockout samples were subjected to SDS-PAGE. ab68194 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (ab68194)

    Predicted band size: 103 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com