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Rabbit Recombinant Monoclonal alpha Actinin/ACTN1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (AB81265), expandable thumbnail
  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (AB81265), expandable thumbnail
  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (AB81265), expandable thumbnail
  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (AB81265), expandable thumbnail
  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (AB81265), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IF
Human
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Tested
Not recommended
Rat
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Rat
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Human
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Mouse
Dilution info
1/500 - 1/1000
Notes

-

Species
Rat
Dilution info
1/500 - 1/1000
Notes

-

Species
Human
Dilution info
1/500 - 1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (PubMed:22689882).

Alternative names

Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1

Recommended products

Rabbit Recombinant Monoclonal alpha Actinin/ACTN1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP2528Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha Actinin-1 also known as ACTN1 or Actn 1 is a cytoskeletal protein that cross-links actin filaments in muscle and non-muscle cells. With a mass of approximately 100 kDa it is an important component of cell structures such as Z-discs in skeletal muscle. Alpha Actinin is expressed in a range of tissues including skeletal cardiac and smooth muscle as well as in various non-muscle cells. Its main role is to organize the actin cytoskeleton providing structural support and enabling cell motility.

Biological function summary

Alpha Actinin-1 helps maintain the structural integrity of cells by anchoring actin filaments and connecting them to integrins or membrane proteins. It often functions as part of a protein complex interacting with other cytoskeletal components and signaling molecules to stabilize cellular structures. In muscle cells it blends with titin and other sarcomeric proteins to assist muscle contraction. Beyond structural roles it partakes in cellular signaling pathways by interacting with signaling proteins and influencing cellular responses to external stimuli.

Pathways

Alpha Actinin-1 is involved in the regulation of the actin cytoskeleton pathway as well as the integrin signaling pathway. In these pathways it collaborates with proteins such as vinculin and paxillin modulating cell adhesion and movement. Its ability to interact with integrin-linked kinases suggests a role in transducing mechanical signals from the extracellular matrix to downstream signaling cascades.

Associated diseases and disorders

Alpha Actinin-1 has been linked to a spectrum of muscular diseases and certain cancers. Mutations in Alpha Actinin-1 can contribute to hereditary diseases like congenital kidney disorders which affect actin filament organization in podocytes. In oncology altered expression levels of Alpha Actinin-1 can affect cell proliferation impacting tumor growth and metastasis. Its interaction with other proteins like focal adhesion kinase (FAK) underlines its involvement in these pathological conditions influencing cellular adhesion and signal transduction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265), expandable thumbnail

    Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265)

    Alpha Actinin/ACTN1 Western blot staining using rabbit Anti-alpha Actinin/ACTN1 antibody

    Lanes 1 - 4: Merged signal (red and green). Green - ab81265 observed at 115 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

    ab81265 was shown to react with ACTN1 in wild-type HAP1 cells in western blot with loss of signal observed in ACTN1 knockout sample. Wild-type and ACTN1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab81265 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265) at 1/1000 dilution

    Lane 1: Wild-type HAP1 cell lysate at 20 µg

    Lane 2: ACTN1 knockout HAP1 cell lysate at 20 µg

    Lane 3: A431 cell lysate at 20 µg

    Lane 4: Daudi cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 103 kDa

    Observed band size: 115 kDa

  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265), expandable thumbnail

    Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265)

    Alpha Actinin/ACTN1 Western blot staining using rabbit Anti-alpha Actinin/ACTN1 antibody

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: Alpha Actinin knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: NIH 3T3 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab81265 observed at 103 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab81265 was shown to recognize alpha Actinin in wild-type HAP1 cells as signal was lost at the expected MW in alpha Actinin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and alpha Actinin knockout samples were subjected to SDS-PAGE. ab81265 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265)

    Predicted band size: 103 kDa

  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265), expandable thumbnail

    Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265)

    Alpha Actinin/ACTN1 Western blot staining using rabbit Anti-alpha Actinin/ACTN1 antibody

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265) at 1/1000 dilution

    Lane 1: NIH/3T3 whole cell lysate at 20 µg

    Lane 2: C2C12 whole cell lysate at 20 µg

    Lane 3: C6 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 103 kDa

    Observed band size: 103 kDa

  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265), expandable thumbnail

    Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265)

    Alpha Actinin/ACTN1 Western blot staining of A673 whole cell lysate using rabbit Anti-alpha Actinin/ACTN1 antibody

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265) at 1/1000 dilution

    All lanes: A673 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 103 kDa

    Observed band size: 103 kDa

  • Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265), expandable thumbnail

    Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265)

    Alpha Actinin/ACTN1 Western blot staining using rabbit Anti-alpha Actinin/ACTN1 antibody

    All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - Loading Control (ab81265) at 1/1000 dilution

    Lane 1: NIH 3T3 cell lysate at 10 µg

    Lane 2: human skeletal muscle tissue lysate at 10 µg

    Lane 3: human heart tissue lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 103 kDa

    Observed band size: 103 kDa

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Product protocols

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