Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (ab90421)

Mouse Monoclonal alpha Actinin/ACTN1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ACTN1 aa 650 to C-terminus.

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Images

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421), expandable thumbnail

Publications

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Fragment Protein within Human ACTN1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P12814

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF
Human	Tested	Tested	Tested
Mouse	Expected	Tested	Expected
Rat	Expected	Tested	Expected
African green monkey	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/200.00000 - 1/2000.00000	Notes -
Species Human	Dilution info 1/200.00000 - 1/2000.00000	Notes -
Species Rat	Dilution info 1/200.00000 - 1/2000.00000	Notes -
Species African green monkey	Dilution info 1/200.00000 - 1/2000.00000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information ACTN1

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (PubMed:22689882).

Alternative names

Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Fragment Protein within Human ACTN1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P12814
Clone number: OTI7A4
Purification technique: Affinity purification
Concentration
Purification notes: Purified from cell culture supernatant by affinity chromatography

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

The clone number has been updated from 7A4 to OTI7A4, both clone numbers name the same clone.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Alpha Actinin-1 also known as ACTN1 or Actn 1 is a cytoskeletal protein that cross-links actin filaments in muscle and non-muscle cells. With a mass of approximately 100 kDa it is an important component of cell structures such as Z-discs in skeletal muscle. Alpha Actinin is expressed in a range of tissues including skeletal cardiac and smooth muscle as well as in various non-muscle cells. Its main role is to organize the actin cytoskeleton providing structural support and enabling cell motility.

Biological function summary

Alpha Actinin-1 helps maintain the structural integrity of cells by anchoring actin filaments and connecting them to integrins or membrane proteins. It often functions as part of a protein complex interacting with other cytoskeletal components and signaling molecules to stabilize cellular structures. In muscle cells it blends with titin and other sarcomeric proteins to assist muscle contraction. Beyond structural roles it partakes in cellular signaling pathways by interacting with signaling proteins and influencing cellular responses to external stimuli.

Pathways

Alpha Actinin-1 is involved in the regulation of the actin cytoskeleton pathway as well as the integrin signaling pathway. In these pathways it collaborates with proteins such as vinculin and paxillin modulating cell adhesion and movement. Its ability to interact with integrin-linked kinases suggests a role in transducing mechanical signals from the extracellular matrix to downstream signaling cascades.

Associated diseases and disorders

Alpha Actinin-1 has been linked to a spectrum of muscular diseases and certain cancers. Mutations in Alpha Actinin-1 can contribute to hereditary diseases like congenital kidney disorders which affect actin filament organization in podocytes. In oncology altered expression levels of Alpha Actinin-1 can affect cell proliferation impacting tumor growth and metastasis. Its interaction with other proteins like focal adhesion kinase (FAK) underlines its involvement in these pathological conditions influencing cellular adhesion and signal transduction.

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9 product images

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (ab90421)
Alpha Actinin/ACTN1 Western blot staining using mouse Anti-alpha Actinin/ACTN1 antibody
All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (ab90421) at 1/200 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) extracts at 10 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts at 10 µg
Lane 3: SVT2 (Mouse cell line) extracts at 10 µg
Lane 4: A549 (Human lung carcinoma cell line) extracts at 10 µg
Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) extracts at 10 µg
Lane 6: Jurkat (Human T cell leukemia cell line from peripheral blood) extracts at 10 µg
Lane 7: PC-12 (Rat adrenal gland pheochromocytoma cell line) extracts at 10 µg
Lane 8: MCF7 (Human breast adenocarcinoma cell line) extracts at 10 µg
Predicted band size: 103 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)
Paraffin-embedded human breast tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Actinin/ATCN1 using ab90421 at a 1/100 dilution in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)
Paraffin-embedded human bladder tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)
Paraffin-embedded human ovary tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)
Paraffin-embedded human liver tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)
pCMV6-ENTRY alpha Actinin/ATCN1 cDNA transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Actinin/ATCN1 using ab90421 at a 1/100 dilution in ICC/IF.
Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (ab90421)
Alpha Actinin/ACTN1 Western blot staining using mouse Anti-alpha Actinin/ACTN1 antibody
All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (ab90421) at 1/200 dilution
Lane 1: NIH/3T3 (Mouse embryo fibroblast cell line) extracts at 10 µg
Lane 2: Mouse brain tissue extracts at 10 µg
Lane 3: Mouse liver tissue extracts at 10 µg
Lane 4: Mouse spleen tissue extracts at 10 µg
Predicted band size: 103 kDa
Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (ab90421)
Alpha Actinin/ACTN1 Western blot staining using mouse Anti-alpha Actinin/ACTN1 antibody
All lanes: Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (ab90421) at 1/2000 dilution
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were transfected with the pCMV6-ENTRY control cDNA at 5 µg
Lane 2: HEK-293T cells were transfected with pCMV6-ENTRY alpha Actinin/ATCN1 cDNA at 5 µg
Predicted band size: 103 kDa