AB90421

Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control

Lab Essentials
What is this?

Be the first to review this product! Submit a review

(6 Publications)

Mouse Monoclonal alpha Actinin/ACTN1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, African green monkey samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTN1 aa 650 to C-terminus.

View Alternative Names

Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1

9 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Actinin/ATCN1 using ab90421 at a 1/100 dilution in ICC/IF.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

pCMV6-ENTRY alpha Actinin/ATCN1 cDNA transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Actinin/ATCN1 using ab90421 at a 1/100 dilution in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Paraffin-embedded human liver tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Paraffin-embedded human ovary tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Paraffin-embedded human bladder tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Paraffin-embedded human breast tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Supplier Data

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

All lanes:

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (ab90421) at 1/2000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were transfected with the pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cells were transfected with pCMV6-ENTRY alpha Actinin/ATCN1 cDNA at 5 µg

Predicted band size: 103 kDa

false

Supplier Data

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

All lanes:

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (ab90421) at 1/200 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) extracts at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts at 10 µg

Lane 3:

SVT2 (Mouse cell line) extracts at 10 µg

Lane 4:

A549 (Human lung carcinoma cell line) extracts at 10 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) extracts at 10 µg

Lane 6:

Jurkat (Human T cell leukemia cell line from peripheral blood) extracts at 10 µg

Lane 7:

PC-12 (Rat adrenal gland pheochromocytoma cell line) extracts at 10 µg

Lane 8:

MCF7 (Human breast adenocarcinoma cell line) extracts at 10 µg

Predicted band size: 103 kDa

false

Supplier Data

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

All lanes:

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (ab90421) at 1/200 dilution

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) extracts at 10 µg

Lane 2:

Mouse brain tissue extracts at 10 µg

Lane 3:

Mouse liver tissue extracts at 10 µg

Lane 4:

Mouse spleen tissue extracts at 10 µg

Predicted band size: 103 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI7A4

Isotype

IgG2a

Carrier free

Reacts with

Mouse, Rat, Human, African green monkey

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ACTN1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P12814

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

The clone number has been updated from 7A4 to OTI7A4, both clone numbers name the same clone.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Actinin-1 also known as ACTN1 or Actn 1 is a cytoskeletal protein that cross-links actin filaments in muscle and non-muscle cells. With a mass of approximately 100 kDa it is an important component of cell structures such as Z-discs in skeletal muscle. Alpha Actinin is expressed in a range of tissues including skeletal cardiac and smooth muscle as well as in various non-muscle cells. Its main role is to organize the actin cytoskeleton providing structural support and enabling cell motility.

Biological function summary

Alpha Actinin-1 helps maintain the structural integrity of cells by anchoring actin filaments and connecting them to integrins or membrane proteins. It often functions as part of a protein complex interacting with other cytoskeletal components and signaling molecules to stabilize cellular structures. In muscle cells it blends with titin and other sarcomeric proteins to assist muscle contraction. Beyond structural roles it partakes in cellular signaling pathways by interacting with signaling proteins and influencing cellular responses to external stimuli.

Pathways

Alpha Actinin-1 is involved in the regulation of the actin cytoskeleton pathway as well as the integrin signaling pathway. In these pathways it collaborates with proteins such as vinculin and paxillin modulating cell adhesion and movement. Its ability to interact with integrin-linked kinases suggests a role in transducing mechanical signals from the extracellular matrix to downstream signaling cascades.

Alpha Actinin-1 has been linked to a spectrum of muscular diseases and certain cancers. Mutations in Alpha Actinin-1 can contribute to hereditary diseases like congenital kidney disorders which affect actin filament organization in podocytes. In oncology altered expression levels of Alpha Actinin-1 can affect cell proliferation impacting tumor growth and metastasis. Its interaction with other proteins like focal adhesion kinase (FAK) underlines its involvement in these pathological conditions influencing cellular adhesion and signal transduction.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (PubMed : 22689882).

See full target information ACTN1

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 45:551-566 PubMed39759533

2025

Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hong Wang,Ting-Ting Liu,Yan-Ping Guo,Shuo-Ji Zhu,Zi-Ming Liao,Jia-Mei Song,Xi-Ming Zhu,Jia-Liang Liang,Moussa Ide Nasser,Nan-Bo Liu,De-Hua Chang,Ping Zhu,Bin Yao

Journal of cellular and molecular medicine 28:e70022 PubMed39205384

2024

Lactate regulates pathological cardiac hypertrophy via histone lactylation modification.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai-Shuai Zhao,Jinlong Liu,Qi-Cai Wu,Xue-Liang Zhou

Immunity, inflammation and disease 11:e835 PubMed37647427

2023

TFRC in cardiomyocytes promotes macrophage infiltration and activation during the process of heart failure through regulating Ccl2 expression mediated by hypoxia inducible factor-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyun Pan,Jinxiu Yang,Jin Dai,Xiaoming Xu,Xinbin Zhou,Wei Mao

Open medicine (Warsaw, Poland) 18:20230681 PubMed37197359

2023

Long noncoding RNA alleviates angiotensin II-induced cardiac hypertrophy phenotypes by mediating the miR-765/Wnt family member 7B pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Manli Yuan,Huaping Jia,Bei Zhao,Can Zhang,Xiaowen Zuo

Journal of translational medicine 21:290 PubMed37120549

2023

OSMR deficiency aggravates pressure overload-induced cardiac hypertrophy by modulating macrophages and OSM/LIFR/STAT3 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhou Feng,Yuan Yuan,Hongxia Xia,Zhaopeng Wang,Yan Che,Zhefu Hu,Jiangyang Deng,Fangfang Li,Qingqing Wu,Zhouyan Bian,Heng Zhou,Difei Shen,Qizhu Tang

The Journal of biological chemistry 287:15275-83 PubMed22427672

2012

Substrate-selective and calcium-independent activation of CaMKII by α-actinin.

Applications

Unspecified application

Species

Unspecified reactive species

Nidhi Jalan-Sakrikar,Ryan K Bartlett,Anthony J Baucum,Roger J Colbran

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control

Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] - Loading Control (AB90421)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise