Rabbit Polyclonal alpha Adaptin antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human AP2A2.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human AP2A2. The exact immunogen used to generate this antibody is proprietary information. Database link O94973
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Associated Products
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Target data
Function
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed:23676497). The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).
Alternative names
ADTAB, CLAPA2, HIP9, HYPJ, KIAA0899, AP2A2, AP-2 complex subunit alpha-2, 100 kDa coated vesicle protein C, Adaptor protein complex AP-2 subunit alpha-2, Adaptor-related protein complex 2 subunit alpha-2, Alpha-adaptin C, Alpha2-adaptin, Clathrin assembly protein complex 2 alpha-C large chain, Huntingtin yeast partner J, Huntingtin-interacting protein 9, Huntingtin-interacting protein J, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit, HIP-9
Recommended products
Rabbit Polyclonal alpha Adaptin antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human AP2A2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human AP2A2. The exact immunogen used to generate this antibody is proprietary information. Database link O94973
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha adaptin also known as AP2A1 is a component of the AP-2 complex carrying a molecular mass of about 110 kDa. This protein functions mechanically by mediating the formation of clathrin-coated vesicles at the plasma membrane. It facilitates the cargo selection process in clathrin-mediated endocytosis. Alpha adaptin is expressed broadly with significant presence in tissues like the brain and kidneys. This protein interacts strongly with clathrin and cargo receptors to regulate the internalization of various membrane-bound molecules.
- Biological function summary
Alpha adaptin contributes to cellular homeostasis by managing the uptake and recycling of receptors and nutrients. It is an integral part of the adaptor protein complex 2 (AP-2) which orchestrates cargo binding and vesicle formation on the plasma membrane. This complex includes other subunits like beta adaptin mu adaptin and sigma adaptin all working collaboratively to ensure proper endocytic function. Alpha adaptin's role is essential in maintaining cell surface receptor levels and nutrient uptake.
- Pathways
This protein is involved in endocytic pathways and is critical in synaptic vesicle recycling. Through this process it regulates neurotransmitter release by facilitating synaptic vesicle endocytosis a vital mechanism in neuron function. Alpha adaptin also interacts with dynamin another significant player in clathrin-mediated endocytosis which pinches off vesicles from the plasma membrane. This interaction supports its role in vesicle trafficking and cellular communication.
- Associated diseases and disorders
Alpha adaptin has implications in neurological disorders and cancer. Dysregulation of clathrin-mediated endocytosis involving alpha adaptin can influence the pathogenesis of neurodegenerative diseases like Alzheimer's disease. Perturbations in the AP-2 complex including alpha adaptin can misregulate receptor internalization impacting cell proliferation and migration therefore having potential links with oncogenesis. Moreover its interaction with proteins involved in receptor signaling pathways highlights its relevance to these conditions.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-alpha Adaptin antibody (ab186828)
All lanes: Western blot - Anti-alpha Adaptin antibody (ab186828) at 1/500 dilution
Lane 1: 22RV1 cell extracts
Lane 2: SK0V3 cell extracts
Lane 3: Mouse brain cell extracts
Predicted band size: 108 kDa
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