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Mouse Monoclonal AP-2 complex subunit alpha-1 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human, Mouse samples. Cited in 28 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (AB2730), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (AB2730), expandable thumbnail
  • Flow Cytometry - Anti-alpha Adaptin antibody [AP6] (AB2730), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (AB2730), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (AB2730), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Human
Tested
Tested
Mouse
Expected
Tested
Primates
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Primates

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

5 µg/mL

Notes

-

Species

Human

Dilution info

5 µg/mL

Notes

-

Predicted
Predicted

Species

Primates

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed:23676497). The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).

Targets

AP-2 complex subunit alpha-2

Alternative names

Recommended products

Mouse Monoclonal AP-2 complex subunit alpha-1 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human, Mouse samples. Cited in 28 publications.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Clone number

AP6

Purification technique

Affinity purification

Specificity

Detects assembly polypeptide 2 (AP2) It recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha A found in neurons.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Alpha adaptin also known as AP2A1 is a component of the AP-2 complex carrying a molecular mass of about 110 kDa. This protein functions mechanically by mediating the formation of clathrin-coated vesicles at the plasma membrane. It facilitates the cargo selection process in clathrin-mediated endocytosis. Alpha adaptin is expressed broadly with significant presence in tissues like the brain and kidneys. This protein interacts strongly with clathrin and cargo receptors to regulate the internalization of various membrane-bound molecules.

Biological function summary

Alpha adaptin contributes to cellular homeostasis by managing the uptake and recycling of receptors and nutrients. It is an integral part of the adaptor protein complex 2 (AP-2) which orchestrates cargo binding and vesicle formation on the plasma membrane. This complex includes other subunits like beta adaptin mu adaptin and sigma adaptin all working collaboratively to ensure proper endocytic function. Alpha adaptin's role is essential in maintaining cell surface receptor levels and nutrient uptake.

Pathways

This protein is involved in endocytic pathways and is critical in synaptic vesicle recycling. Through this process it regulates neurotransmitter release by facilitating synaptic vesicle endocytosis a vital mechanism in neuron function. Alpha adaptin also interacts with dynamin another significant player in clathrin-mediated endocytosis which pinches off vesicles from the plasma membrane. This interaction supports its role in vesicle trafficking and cellular communication.

Associated diseases and disorders

Alpha adaptin has implications in neurological disorders and cancer. Dysregulation of clathrin-mediated endocytosis involving alpha adaptin can influence the pathogenesis of neurodegenerative diseases like Alzheimer's disease. Perturbations in the AP-2 complex including alpha adaptin can misregulate receptor internalization impacting cell proliferation and migration therefore having potential links with oncogenesis. Moreover its interaction with proteins involved in receptor signaling pathways highlights its relevance to these conditions.

Product promise

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5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

    Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in U251 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

    Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2720) shows staining in MCF-7 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4 C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Flow Cytometry - Anti-alpha Adaptin antibody [AP6] (ab2730), expandable thumbnail

    Flow Cytometry - Anti-alpha Adaptin antibody [AP6] (ab2730)

    Overlay histogram showing MCF7 cells stained with ab2730 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2730, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

    Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in Hela Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2720) at a dilution of 1:20 over night at 4 C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

    ICC/IF image of ab2730 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2730, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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