AB51130

Anti-alpha Adducin antibody

Name: Anti-alpha Adducin antibody (ab51130) | Abcam
Brand: Abcam Limited
SKU: AB51130
Price: 435 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(16 Publications)

Rabbit Polyclonal alpha Adducin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human ADD1.

View Alternative Names

ADDA, ADD1, Alpha-adducin, Erythrocyte adducin subunit alpha

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody (AB51130)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody (AB51130)

This image shows paraffin-embedded human brain tissue stained with ab51130 at a dilution of 1/100. Right hand image : tissue treated with immunising peptide; left hand image : untreated tissue

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin antibody (AB51130)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin antibody (AB51130)

ICC/IF image of ab51130 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-alpha Adducin antibody (AB51130)

All lanes:

Western blot - Anti-alpha Adducin antibody (ab51130)

Lane 1:

Extracts from Hela cells treated with Forskolin (40nM, 30min) with no peptide

Lane 2:

Extracts from Hela cells treated with Forskolin (40nM, 30min) with with immunising peptide

Predicted band size: 81 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ADD1. The exact immunogen used to generate this antibody is proprietary information.

P35611

Specificity

There are 2 isoforms of alpha adducin on the Uniprot protein database page for mouse. The antibody immunogen has high alignment to isoform 1: Isoform 1 80 kDa 100% Isoform 2 69 kDa 30% The aduccin protein is made up of 4 subunits, including the alpha beta and gamma subunits (according to the Uniprot page), Alignment of the immunogen with the mouse beta adducin: http://www.uniprot.org/uniprot/Q9QYB8 isoform 1 100% isoform 2 30% isoform 3 100% And with the mouse gamma adducin: http://www.uniprot.org/uniprot/Q9QYB5 isoform 1 92% Isoform 2 92% The antibody has not been specifically tested for detection of the other subunits, however according to the high alignments, the antibody is likely to detect several isoforms of these beta and gamma subunits.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Adducin also known as ADD1 is a cytoskeletal protein of roughly 80 kDa. It plays a significant mechanical role in the assembly of the spectrin-actin network which stabilizes the membrane of erythrocytes and other cells. It is expressed in various tissues with high levels in neurons and kidney cells. Maintenance of cytoskeletal integrity is an important mechanical function of alpha Adducin.

Biological function summary

This protein influences cellular shape and motility. It forms a complex with spectrin and actin to strengthen cell membranes and facilitate intercellular connectivity. Alpha Adducin modulates the dynamics of membrane skeleton structures impacting not only the stability but also the function of cells under both physiological and pathophysiological conditions.

Pathways

Alpha Adducin is integral in the regulation of the Rho and calcium signaling pathways. These pathways influence cytoskeletal organization and cell life processes. In the Rho pathway alpha Adducin interacts with proteins such as Rho GTPases which are involved in signal transduction for cytoskeletal rearrangement. This interaction shows its importance in cellular reaction to environmental stimuli and stress.

Alpha Adducin shows significant connections to hypertension and certain neurological disorders. Variants of ADD1 have been linked to altered blood pressure regulation implicating its role in hypertension through interactions with sodium-potassium ATPases. It also connects to spectrin in neurological disorders highlighting its involvement in maintaining neuronal architecture and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

See full target information ADD1

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Science (New York, N.Y.) 389:eadn6712 PubMed40773558

2025

The membrane skeleton is constitutively remodeled in neurons by calcium signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Evan Heller,Naina Kurup,Xiaowei Zhuang

Science advances 11:eadt3724 PubMed40053592

2025

Paralemmin-1 controls the nanoarchitecture of the neuronal submembrane cytoskeleton.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Macarrón-Palacios,Jasmine Hubrich,Maria Augusta do Rego Barros Fernandes Lima,Nicole G Metzendorf,Simon Kneilmann,Marleen Trapp,Claudio Acuna,Annarita Patrizi,Elisa D'Este,Manfred W Kilimann

Cell reports methods 3:100571 PubMed37751691

2023

Assessing crosstalk in simultaneous multicolor single-molecule localization microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Karoline Friedl,Adrien Mau,Fanny Boroni-Rueda,Valentina Caorsi,Nicolas Bourg,Sandrine Lévêque-Fort,Christophe Leterrier

Cell genomics 3:100250 PubMed36950384

2023

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Greta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper Lage

Scientific reports 12:14940 PubMed36056066

2022

Lack of adducin impairs the stability of endothelial adherens and tight junctions and may be required for cAMP-Rac1-mediated endothelial barrier stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Sina Moztarzadeh,Mariya Y Radeva,Sara Sepic,Katharina Schuster,Ibrahim Hamad,Jens Waschke,Alexander García-Ponce

eLife 9: PubMed32195665

2020

The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Rita Costa,Sara C Sousa,Rita Pinto-Costa,José C Mateus,Cátia Df Lopes,Ana Catarina Costa,David Rosa,Diana Machado,Luis Pajuelo,Xuewei Wang,Feng-Quan Zhou,António J Pereira,Paula Sampaio,Boris Y Rubinstein,Inês Mendes Pinto,Marko Lampe,Paulo Aguiar,Monica M Sousa

eLife 8: PubMed31042147

2019

Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Guiping Wang,David J Simon,Zhuhao Wu,Deanna M Belsky,Evan Heller,Melanie K O'Rourke,Nicholas T Hertz,Henrik Molina,Guisheng Zhong,Marc Tessier-Lavigne,Xiaowei Zhuang

Nature communications 10:1980 PubMed31040275

2019

Combining 3D single molecule localization strategies for reproducible bioimaging.

Applications

Unspecified application

Species

Unspecified reactive species

Clément Cabriel,Nicolas Bourg,Pierre Jouchet,Guillaume Dupuis,Christophe Leterrier,Aurélie Baron,Marie-Ange Badet-Denisot,Boris Vauzeilles,Emmanuel Fort,Sandrine Lévêque-Fort

Cell reports 24:1512-1522 PubMed30089262

2018

The Spectrin-Actin-Based Periodic Cytoskeleton as a Conserved Nanoscale Scaffold and Ruler of the Neural Stem Cell Lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Meghan Hauser,Rui Yan,Wan Li,Nicole A Repina,David V Schaffer,Ke Xu

Journal of cell science 131: PubMed29739876

2018

The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure.

Applications

Unspecified application

Species

Unspecified reactive species

María G Gervasi,Xinran Xu,Blanca Carbajal-Gonzalez,Mariano G Buffone,Pablo E Visconti,Diego Krapf

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-alpha Adducin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody (AB51130)

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin antibody (AB51130)

Western blot - Anti-alpha Adducin antibody (AB51130)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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