AB53093

Anti-alpha Adducin (phospho S726) antibody

Name: Anti-alpha Adducin (phospho S726) antibody (ab53093) | Abcam
Brand: Abcam Limited
SKU: AB53093
Price: 520 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal alpha Adducin phospho S726 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ADD1 pS726 aa 650-750.

View Alternative Names

ADDA, ADD1, Alpha-adducin, Erythrocyte adducin subunit alpha

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody (AB53093)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody (AB53093)

Immunocytochemistry analysis of HeLa cells, using ab53093 at 1/100 dilution. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (AB53093)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (AB53093)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ab53093 at 1/50. The picture on the right is blocked with the phospo peptide.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (AB53093)

labelled with ab53093, at 1/50 dilution, staining alpha Adducin in paraffin embedded human brain tissue sections by Immunohistochemistry. Tissues had either been treated with (right image) or without (left image) the phosphopeptide.

Supplier Data

Western blot - Anti-alpha Adducin (phospho S726) antibody (AB53093)

All lanes:

Western blot - Anti-alpha Adducin (phospho S726) antibody (ab53093) at 1/500 dilution

Lane 1:

HeLa cells treated with Forskolin (40nM 30 mins)

Lane 2:

HeLa cells treated with Forskolin (40nM 30 mins) and blocked with the phospho peptide.

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ADD1 pS726 aa 650-750. The exact immunogen used to generate this antibody is proprietary information.

P35611

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab53093 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Adducin also known as ADD1 is a cytoskeletal protein of roughly 80 kDa. It plays a significant mechanical role in the assembly of the spectrin-actin network which stabilizes the membrane of erythrocytes and other cells. It is expressed in various tissues with high levels in neurons and kidney cells. Maintenance of cytoskeletal integrity is an important mechanical function of alpha Adducin.

Biological function summary

This protein influences cellular shape and motility. It forms a complex with spectrin and actin to strengthen cell membranes and facilitate intercellular connectivity. Alpha Adducin modulates the dynamics of membrane skeleton structures impacting not only the stability but also the function of cells under both physiological and pathophysiological conditions.

Pathways

Alpha Adducin is integral in the regulation of the Rho and calcium signaling pathways. These pathways influence cytoskeletal organization and cell life processes. In the Rho pathway alpha Adducin interacts with proteins such as Rho GTPases which are involved in signal transduction for cytoskeletal rearrangement. This interaction shows its importance in cellular reaction to environmental stimuli and stress.

Alpha Adducin shows significant connections to hypertension and certain neurological disorders. Variants of ADD1 have been linked to altered blood pressure regulation implicating its role in hypertension through interactions with sodium-potassium ATPases. It also connects to spectrin in neurological disorders highlighting its involvement in maintaining neuronal architecture and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

See full target information ADD1 pS726

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature microbiology 10:1463-1483 PubMed40389650

2025

The fast-evolving FIKK kinase family of Plasmodium falciparum can be inhibited by a single compound.

Applications

Unspecified application

Species

Unspecified reactive species

Hugo Belda,David Bradley,Evangelos Christodoulou,Stephanie D Nofal,Malgorzata Broncel,David Jones,Heledd Davies,M Teresa Bertran,Andrew G Purkiss,Roksana W Ogrodowicz,Dhira Joshi,Nicola O'Reilly,Louise Walport,Andrew Powell,David House,Svend Kjaer,Antoine Claessens,Christian R Landry,Moritz Treeck

Biomolecules 14: PubMed38254651

2024

Piezo1 Regulation Involves Lipid Domains and the Cytoskeleton and Is Favored by the Stomatocyte-Discocyte-Echinocyte Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Amaury Stommen,Marine Ghodsi,Anne-Sophie Cloos,Louise Conrard,Andra C Dumitru,Patrick Henriet,Christophe E Pierreux,David Alsteens,Donatienne Tyteca

Brain pathology (Zurich, Switzerland) 31:e12996 PubMed34218486

2021

Dysregulated protein phosphorylation: A determining condition in the continuum of brain aging and Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Isidro Ferrer,Pol Andrés-Benito,Karina Ausín,Reinald Pamplona,José Antonio Del Rio,Joaquín Fernández-Irigoyen,Enrique Santamaría

Investigative ophthalmology & visual science 50:452-61 PubMed18757509

2008

Efficacy of Rho-kinase inhibition in promoting cell survival and reducing reactive gliosis in the rodent retina.

Applications

Unspecified application

Species

Unspecified reactive species

Aysegül Tura,Frank Schuettauf,Philippe P Monnier,Karl U Bartz-Schmidt,Sigrid Henke-Fahle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Adducin (phospho S726) antibody

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody (AB53093)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (AB53093)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (AB53093)

Western blot - Anti-alpha Adducin (phospho S726) antibody (AB53093)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise