AB40760

Anti-alpha Adducin (phospho S726) antibody [EP734Y]

RabMAb
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Monoclonal alpha Adducin phospho S726 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

ADDA, ADD1, Alpha-adducin, Erythrocyte adducin subunit alpha

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (AB40760)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (AB40760)

ab40760 (1/250), staining human lung by immunohistochemistry, paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (AB40760)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (AB40760)

ab40760, (1/250) staining HeLa cells by immunofluorescence.

Unknown

Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (AB40760)

All lanes:

Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760) at 1/50000 dilution

All lanes:

HeLa cell lysate at 10 µg

Predicted band size: 81 kDa

Observed band size: 120 kDa

false

Lab

Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (AB40760)

Blocking and diluting buffer : 5% NFDM/TBST

Lanes 1 - 3:

Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/alpha-adducin-phospho-s726-antibody-ep734y-bsa-and-azide-free-ab284680'>ab284680</a>)

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate 20ug, then the membrane was treated with alkaline phosphatase for 1 hour at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Observed band size: 110 kDa

false

Exposure time: 7s

Carrier free

Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP734Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Adducin also known as ADD1 is a cytoskeletal protein of roughly 80 kDa. It plays a significant mechanical role in the assembly of the spectrin-actin network which stabilizes the membrane of erythrocytes and other cells. It is expressed in various tissues with high levels in neurons and kidney cells. Maintenance of cytoskeletal integrity is an important mechanical function of alpha Adducin.

Biological function summary

This protein influences cellular shape and motility. It forms a complex with spectrin and actin to strengthen cell membranes and facilitate intercellular connectivity. Alpha Adducin modulates the dynamics of membrane skeleton structures impacting not only the stability but also the function of cells under both physiological and pathophysiological conditions.

Pathways

Alpha Adducin is integral in the regulation of the Rho and calcium signaling pathways. These pathways influence cytoskeletal organization and cell life processes. In the Rho pathway alpha Adducin interacts with proteins such as Rho GTPases which are involved in signal transduction for cytoskeletal rearrangement. This interaction shows its importance in cellular reaction to environmental stimuli and stress.

Alpha Adducin shows significant connections to hypertension and certain neurological disorders. Variants of ADD1 have been linked to altered blood pressure regulation implicating its role in hypertension through interactions with sodium-potassium ATPases. It also connects to spectrin in neurological disorders highlighting its involvement in maintaining neuronal architecture and function.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

See full target information ADD1 pS726

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40867839

2025

Biomarker-Driven Optimization of Saponin Therapy in MASLD: From Mouse Models to Human Liver Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Young Kim,Ju Hee Oh,Hyun Sung Kim,Dae Won Jun

Cancer science 114:1912-1928 PubMed36637351

2023

PRMT3 regulates the progression of invasive micropapillary carcinoma of the breast.

Applications

Unspecified application

Species

Unspecified reactive species

Renyong Zhi,Kailiang Wu,Jingyue Zhang,Hanjiao Liu,Chen Niu,Shuai Li,Li Fu

Cell 185:3390-3407.e18 PubMed36055200

2022

A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Hsien Sheu,Srigokul Upadhyayula,Vincent Dupuy,Song Pang,Fei Deng,Jinxia Wan,Deepika Walpita,H Amalia Pasolli,Justin Houser,Silvia Sanchez-Martinez,Sebastian E Brauchi,Sambashiva Banala,Melanie Freeman,C Shan Xu,Tom Kirchhausen,Harald F Hess,Luke Lavis,Yulong Li,Séverine Chaumont-Dubel,David E Clapham

Frontiers in immunology 10:2706 PubMed31824498

2019

The Actin-Capping Protein Alpha-Adducin Is Required for T-Cell Costimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy J Thauland,Humza A Khan,Manish J Butte

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Adducin (phospho S726) antibody [EP734Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (AB40760)

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (AB40760)

Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (AB40760)

Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (AB40760)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise