Rabbit Monoclonal alpha Adducin phospho S726 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Images
![Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (AB284680), expandable thumbnail](https://content.abcam.com/products/images/alpha-adducin-phospho-s726-antibody-ep734y-bsa-and-azide-free-ab284680--western-blot-img145628.gif "Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (AB284680)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (AB284680), expandable thumbnail](https://content.abcam.com/products/images/alpha-adducin-phospho-s726-antibody-ep734y-bsa-and-azide-free-ab284680--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58645.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (AB284680)")
![Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (AB284680), expandable thumbnail](https://content.abcam.com/products/images/alpha-adducin-phospho-s726-antibody-ep734y-bsa-and-azide-free-ab284680--immunocytochemistry-immunofluorescence-img106349.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (AB284680)")
![Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (AB284680), expandable thumbnail](https://content.abcam.com/products/images/alpha-adducin-phospho-s726-antibody-ep734y-bsa-and-azide-free-ab284680--western-blot-img419431.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (AB284680)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Alternative names
ADDA, ADD1, Alpha-adducin, Erythrocyte adducin subunit alpha
Recommended products
Rabbit Monoclonal alpha Adducin phospho S726 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP734Y
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab284680 is the carrier-free version of Anti-alpha Adducin (phospho S726) antibody [EP734Y] ab40760
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha Adducin also known as ADD1 is a cytoskeletal protein of roughly 80 kDa. It plays a significant mechanical role in the assembly of the spectrin-actin network which stabilizes the membrane of erythrocytes and other cells. It is expressed in various tissues with high levels in neurons and kidney cells. Maintenance of cytoskeletal integrity is an important mechanical function of alpha Adducin.
- Biological function summary
This protein influences cellular shape and motility. It forms a complex with spectrin and actin to strengthen cell membranes and facilitate intercellular connectivity. Alpha Adducin modulates the dynamics of membrane skeleton structures impacting not only the stability but also the function of cells under both physiological and pathophysiological conditions.
- Pathways
Alpha Adducin is integral in the regulation of the Rho and calcium signaling pathways. These pathways influence cytoskeletal organization and cell life processes. In the Rho pathway alpha Adducin interacts with proteins such as Rho GTPases which are involved in signal transduction for cytoskeletal rearrangement. This interaction shows its importance in cellular reaction to environmental stimuli and stress.
- Associated diseases and disorders
Alpha Adducin shows significant connections to hypertension and certain neurological disorders. Variants of ADD1 have been linked to altered blood pressure regulation implicating its role in hypertension through interactions with sodium-potassium ATPases. It also connects to spectrin in neurological disorders highlighting its involvement in maintaining neuronal architecture and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680)
This data was developed using Anti-alpha Adducin (phospho S726) antibody [EP734Y] ab40760, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (Anti-alpha Adducin (phospho S726) antibody [EP734Y] ab40760) at 1/50000 dilution
All lanes: HeLa cell lysate at 10 µg
Predicted band size: 81 kDa
Observed band size: 120 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680), expandable thumbnail](https://content.abcam.com/products/images/alpha-adducin-phospho-s726-antibody-ep734y-bsa-and-azide-free-ab284680--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58645.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680)
This data was developed using Anti-alpha Adducin (phospho S726) antibody [EP734Y] ab40760, the same antibody clone in a different buffer formulation.Anti-alpha Adducin (phospho S726) antibody [EP734Y] ab40760 (1/250), staining human lung by immunohistochemistry, paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680), expandable thumbnail](https://content.abcam.com/products/images/alpha-adducin-phospho-s726-antibody-ep734y-bsa-and-azide-free-ab284680--immunocytochemistry-immunofluorescence-img106349.jpg "Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680)")
Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680)
This data was developed using Anti-alpha Adducin (phospho S726) antibody [EP734Y] ab40760, the same antibody clone in a different buffer formulation.
Anti-alpha Adducin (phospho S726) antibody [EP734Y] ab40760, (1/250) staining HeLa cells by immunofluorescence.
![Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680), expandable thumbnail](https://content.abcam.com/products/images/alpha-adducin-phospho-s726-antibody-ep734y-bsa-and-azide-free-ab284680--western-blot-img419431.jpg "Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680)")
Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680)
Lanes 1 - 3: Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (Anti-alpha Adducin (phospho S726) antibody [EP734Y] ab40760) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680)
Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate at 20 µg
Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate 20ug, then the membrane was treated with alkaline phosphatase for 1 hour at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Observed band size: 110 kDa
Exposure time: 7s
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