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AB182894

Anti-alpha amylase antibody

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(1 Publication)

Rabbit Polyclonal alpha amylase antibody. Suitable for WB, IF and reacts with Bacillus subtilis samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Bacillus subtilis subsp. subtilis str. 168 amyE.

View Alternative Names

amyA, BSU03040, amyE, Alpha-amylase

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Bacillus subtilis

Applications

WB, IF

applications

Immunogen

Native Full Length Protein corresponding to Bacillus subtilis subsp. subtilis str. 168 amyE.

P00691

Specificity

Cross-reactivities against enzymes of other sources may occur but have not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IF" : {"fullname" : "Immunofluorescence", "shortname":"IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bacillus subtilis": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IF-species-checked": "guaranteed", "IF-species-dilution-info": "", "IF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 1mL
Purity
IgG fraction
Storage buffer
pH: 7.2 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha amylase also known as 14-alpha-D-glucan glucanohydrolase is an enzyme that catalyzes the hydrolysis of alpha bonds in large alpha-linked polysaccharides such as starch and glycogen breaking them down into glucose and maltose. The enzyme has a molecular mass that ranges from approximately 50 to 60 kDa depending on the isoform. Alpha amylase is expressed in the salivary glands and pancreas in humans where it initiates the digestive process. Additionally it is present in some microbiota aiding carbohydrate metabolism.
Biological function summary

Alpha amylase plays an important role in carbohydrate digestion breaking down dietary starch into simpler sugars for absorption. Although alpha amylase is not typically part of a larger protein complex its activity complements other enzymes like maltase and isomaltase in the digestive tract. By converting complex carbohydrates into absorbable glucose alpha amylase aids in maintaining energy homeostasis in the body influencing glucose metabolism and energy availability.

Pathways

Alpha amylase is critical in the digestive system's carbohydrate metabolism pathway. This pathway begins in the mouth and continues through the small intestine contributing to overall digestive efficiency. In the context of glucose metabolism alpha amylase works synergistically with enzymes such as glucokinase and glucose-6-phosphatase ensuring glucose levels are effectively managed for cellular energy production. This regulation is essential for maintaining normal blood sugar levels and supporting metabolic health.

Alpha amylase has been linked to conditions like diabetes and pancreatitis. In diabetes abnormal alpha amylase activity can contribute to dysregulated glucose levels complicating metabolic control. In cases of pancreatitis elevated levels of alpha amylase in the blood serve as a biomarker for the disease due to the enzyme's leakage from damaged pancreatic cells. Both conditions can involve interactions with proteins such as insulin in diabetes and trypsin in pancreatitis further influencing the disease mechanisms and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information amyE
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39273244

2024

Strategies to Maintain Redox Homeostasis in Yeast Cells with Impaired Fermentation-Dependent NADPH Generation.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Kwolek-Mirek,Roman Maslanka,Sabina Bednarska,Michał Przywara,Kornelia Kwolek,Renata Zadrag-Tecza
View all publications
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Complementary Products

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Product promise

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