Skip to main content

Rabbit Polyclonal alpha amylase antibody. Suitable for WB, IF and reacts with Bacillus subtilis samples. Immunogen corresponding to Native Full Length Protein corresponding to Bacillus subtilis subsp. subtilis str. 168 amyE.

Be the first to review this product! Submit a review

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Constituents: 100% PBS

Form
Lyophilized
Clonality
Polyclonal

Immunogen

  • Native Full Length Protein corresponding to Bacillus subtilis subsp. subtilis str. 168 amyE. Database link P00691

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIF
Bacillus subtilis
Expected
Expected

Expected
Expected

Species
Bacillus subtilis
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Bacillus subtilis
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Additional Targets

alpha amylase

Alternative names

Recommended products

Rabbit Polyclonal alpha amylase antibody. Suitable for WB, IF and reacts with Bacillus subtilis samples. Immunogen corresponding to Native Full Length Protein corresponding to Bacillus subtilis subsp. subtilis str. 168 amyE.

Key facts

Isotype
IgG
Form
Lyophilized
Clonality
Polyclonal
Immunogen
  • Native Full Length Protein corresponding to Bacillus subtilis subsp. subtilis str. 168 amyE. Database link P00691
Purity
IgG fraction
Specificity

Cross-reactivities against enzymes of other sources may occur but have not been determined.

Reconstitution
Add 1.0 ml sterile distilled water. Spin down to remove insoluble particles.
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha amylase also known as 14-alpha-D-glucan glucanohydrolase is an enzyme that catalyzes the hydrolysis of alpha bonds in large alpha-linked polysaccharides such as starch and glycogen breaking them down into glucose and maltose. The enzyme has a molecular mass that ranges from approximately 50 to 60 kDa depending on the isoform. Alpha amylase is expressed in the salivary glands and pancreas in humans where it initiates the digestive process. Additionally it is present in some microbiota aiding carbohydrate metabolism.

Biological function summary

Alpha amylase plays an important role in carbohydrate digestion breaking down dietary starch into simpler sugars for absorption. Although alpha amylase is not typically part of a larger protein complex its activity complements other enzymes like maltase and isomaltase in the digestive tract. By converting complex carbohydrates into absorbable glucose alpha amylase aids in maintaining energy homeostasis in the body influencing glucose metabolism and energy availability.

Pathways

Alpha amylase is critical in the digestive system's carbohydrate metabolism pathway. This pathway begins in the mouth and continues through the small intestine contributing to overall digestive efficiency. In the context of glucose metabolism alpha amylase works synergistically with enzymes such as glucokinase and glucose-6-phosphatase ensuring glucose levels are effectively managed for cellular energy production. This regulation is essential for maintaining normal blood sugar levels and supporting metabolic health.

Associated diseases and disorders

Alpha amylase has been linked to conditions like diabetes and pancreatitis. In diabetes abnormal alpha amylase activity can contribute to dysregulated glucose levels complicating metabolic control. In cases of pancreatitis elevated levels of alpha amylase in the blood serve as a biomarker for the disease due to the enzyme's leakage from damaged pancreatic cells. Both conditions can involve interactions with proteins such as insulin in diabetes and trypsin in pancreatitis further influencing the disease mechanisms and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com