JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB327632

Anti-alpha Amylase antibody [HL1681] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal AMYP antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Mouse, Rat, Human, Zebrafish samples.

View Alternative Names

Pancreatic alpha-amylase, PA, AMY2A

6 Images
Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Human pancreas (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ab327632 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/1000 dilution

All lanes:

Human pancreas tissue extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with ab327632 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/5000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts

Lane 2:

DDDDK-tagged AMY2A transfected (+) 293T whole cell extracts

Lane 3:

DDDDK-tagged AMY2B transfected (+) 293T whole cell extracts

Lane 4:

DDDDK-tagged AMY1A transfected (+) 293T whole cell extracts

Lane 5:

DDDDK-tagged AMY1B transfected (+) 293T whole cell extracts

Lane 6:

DDDDK-tagged AMY1C transfected (+) 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327632 diluted at 1 : 4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/4000 dilution

Lane 1:

Mouse intestine tissue extracts at 50 µg

Lane 2:

Mouse lung tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted withab327632 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/1000 dilution

Lane 1:

Mouse pancreas tissue extracts at 50 µg

Lane 2:

Mouse lung tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ab327632 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/5000 dilution

All lanes:

Rat pancreas tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ab327632 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1681

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human, Zebrafish

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/4000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Pancreatic alpha-amylase
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com