AB13496

Anti-Alpha B Crystallin antibody [1B6.1-3G4]

Name: Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) | Abcam
Brand: Abcam Limited
SKU: AB13496
Price: 535 USD
Availability: InStock
Rating: 5 (5 reviews)

(5 Reviews)

(68 Publications)

Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) is a mouse monoclonal antibody detecting Alpha B Crystallin in Western Blot, Flow Cytometry, IHC-P. Suitable for Cow, Human.

- Over 50 publications
- Trusted since 2004

View Alternative Names

CRYA2, HSPB5, CRYAB, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Heat shock protein family B member 5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, HspB5

3 Images

Supplier Data

Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (AB13496)

All lanes:

Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) at 1/1000 dilution

Lane 1:

α-Crystallin (bovine), (native) at 1/1000 dilution

Lane 2:

αB-Crystallin (bovine), (native)

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (AB13496)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (AB13496)

Immunohistochemistry (paraffin sections) analysis of Human heart tissue labelling Alpha B Crystallin with ab13496 at 20 ug/mL.

Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (AB13496)

Flow Cyt

Unknown

Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (AB13496)

Overlay histogram showing SH-SY5Y cells stained with ab13496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13496, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1B6.1-3G4

Isotype

IgG1

Carrier free

Reacts with

Human, Cow

Applications

Flow Cyt, IHC-FrFl, WB, IHC-Fr, ICC, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Cow CRYAB.

P02510

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Reactivity data

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Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" }, "Purified native protein - Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "notRecommended", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" }, "Recombinant full length protein": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "notRecommended", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Cow, Human samples.

What is the molecular weight of Alpha B Crystallin?
Anti-Alpha B Crystallin [1B6.1-3G4] (ab13496) specifically detects a band for Alpha B Crystallin (UniProt: P02510) at a molecular weight of 21kDa.

Trusted by the scientific community
Anti-Alpha B Crystallin [1B6.1-3G4] (ab13496) was first used in a scientific publication in 2004 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Alpha B Crystallin [1B6.1-3G4] (ab13496) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha B Crystallin also known as CRYAB or alpha B crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is expressed in various tissues including the eye lens heart skeletal muscles and the brain. Alpha B Crystallin functions mechanically as a chaperone helping to prevent the aggregation of unfolded proteins by stabilizing them. This protein plays an important role in maintaining proper cellular function especially under stress conditions.

Biological function summary

Alpha B crystallin performs as part of the larger small heat shock protein family which contributes to the cellular defense system. It acts primarily as a chaperone binding to denatured proteins to protect the cells from damage during environmental stresses such as heat and oxidative stress. Alpha B crystallin often forms oligomeric complexes which increases its protective properties. This protein exhibits significant anti-apoptotic functions by interacting with various apoptosis-regulating factors contributing to cellular survival.

Pathways

Alpha B crystallin integrates into the cellular stress response and apoptosis pathways. It involves the PI3K/Akt signaling pathway known for regulating cell survival and preventing programmed cell death. Alpha B crystallin also interacts with other proteins such as Bcl-2 further enhancing its role in anti-apoptotic activities. Its contribution to these pathways highlights its protective function during cellular stress situations.

Alpha B crystallin is associated with cataracts and dilated cardiomyopathy. It plays a significant role in maintaining lens transparency and mutations or dysfunctions in alpha B crystallin can lead to cataract development. Similarly in cardiac tissue abnormal alpha B crystallin expression links to dilated cardiomyopathy. The protein is also known to associate with other structural proteins like desmin where disruptions may contribute to muscle-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).

See full target information CRYAB

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Publications (68)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:34552 PubMed41044127

2025

Quantitative spatial analysis of crystallin proteins in human lens epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Cristoforetti,Giorgio Baldessari,Lizaveta Chychko,Ignacio Babiloni Chust,Samuele Sartori,Sonja Schickhardt,Flavia Ravelli,Silvia Bertoluzza,Matthias Carl,Saadettin Sel,Gerd U Auffarth,Lucia Poggi

Addiction biology 30:e70028 PubMed40095747

2025

Crystallin Alpha B Inhibits Cocaine-Induced Conditioned Place Preference via the Modulation of Dopaminergic Neurotransmission.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Mi Gu,Daejin Park,Sowoon Seo,Sanghyeon Kim,Young Eun Kim,Maree J Webster,Heejong Eom,Dohyun Lee,Jin Tae Hong,Sang-Bae Han,Hye Jin Cha,Jaesuk Yun

The Journal of clinical investigation 135: PubMed39932799

2025

Phosphorylation of CRYAB induces a condensatopathy to worsen post-myocardial infarction left ventricular remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Moydul Islam,David R Rawnsley,Xiucui Ma,Walter Navid,Chen Zhao,Xumin Guan,Layla Foroughi,John T Murphy,Honora Navid,Carla J Weinheimer,Attila Kovacs,Jessica Nigro,Aaradhya Diwan,Ryan P Chang,Minu Kumari,Martin E Young,Babak Razani,Kenneth B Margulies,Mahmoud Abdellatif,Simon Sedej,Ali Javaheri,Douglas F Covey,Kartik Mani,Abhinav Diwan

Nature 647:169-178 PubMed39779846

2025

Molecular and cellular dynamics of the developing human neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Cheng Wang,Juan A Moriano,Songcang Chen,Guolong Zuo,Arantxa Cebrián-Silla,Shaobo Zhang,Tanzila Mukhtar,Shaohui Wang,Mengyi Song,Lilian Gomes de Oliveira,Qiuli Bi,Jonathan J Augustin,Xinxin Ge,Mercedes F Paredes,Eric J Huang,Arturo Alvarez-Buylla,Xin Duan,Jingjing Li,Arnold R Kriegstein

International journal of molecular sciences 25: PubMed39596168

2024

Establishment and Use of Primary Cultured Astrocytes from Alexander Disease Model Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuto Kubota,Eiji Shigetomi,Kozo Saito,Youichi Shinozaki,Kenji Kobayashi,Masayoshi Tanaka,Bijay Parajuli,Kenji F Tanaka,Schuichi Koizumi

Science advances 10:eadn9998 PubMed38536915

2024

Multiple parallel cell lineages in the developing mammalian cerebral cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Del-Valle-Anton,Salma Amin,Daniela Cimino,Florian Neuhaus,Elena Dvoretskova,Virginia Fernández,Yigit K Babal,Cristina Garcia-Frigola,Anna Prieto-Colomina,Raquel Murcia-Ramón,Yuki Nomura,Adrián Cárdenas,Chao Feng,Juan Antonio Moreno-Bravo,Magdalena Götz,Christian Mayer,Víctor Borrell

eLife 12: PubMed37988289

2023

Truncated radial glia as a common precursor in the late corticogenesis of gyrencephalic mammals.

Applications

Unspecified application

Species

Unspecified reactive species

Merve Bilgic,Quan Wu,Taeko Suetsugu,Atsunori Shitamukai,Yuji Tsunekawa,Tomomi Shimogori,Mitsutaka Kadota,Osamu Nishimura,Shigehiro Kuraku,Hiroshi Kiyonari,Fumio Matsuzaki

Advanced healthcare materials 13:e2302907 PubMed37797407

2023

Utilization of an Artery-on-a-Chip to Unravel Novel Regulators and Therapeutic Targets in Vascular Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Paloschi,Jessica Pauli,Greg Winski,Zhiyuan Wu,Zhaolong Li,Lorenzo Botti,Sandro Meucci,Pierangelo Conti,Felix Rogowitz,Nadiya Glukha,Nora Hummel,Albert Busch,Ekaterina Chernogubova,Hong Jin,Nadja Sachs,Hans-Henning Eckstein,Anne Dueck,Reinier A Boon,Andreas R Bausch,Lars Maegdefessel

Nature communications 14:5904 PubMed37737269

2023

A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Laddach,Song Hui Chng,Reena Lasrado,Fränze Progatzky,Michael Shapiro,Alek Erickson,Marisol Sampedro Castaneda,Artem V Artemov,Ana Carina Bon-Frauches,Eleni-Maria Amaniti,Jens Kleinjung,Stefan Boeing,Sila Ultanir,Igor Adameyko,Vassilis Pachnis

Frontiers in cardiovascular medicine 10:1223244 PubMed37435054

2023

A novel αB-crystallin R123W variant drives hypertrophic cardiomyopathy by promoting maladaptive calcium-dependent signal transduction.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Chou,Gregory L Martin,Gayani Perera,Junya Awata,Amy Larson,Robert Blanton,Michael T Chin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha B Crystallin antibody [1B6.1-3G4]

Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (AB13496)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (AB13496)

Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (AB13496)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (68)

Product promise