Mouse Monoclonal Alpha B Crystallin antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, African green monkey, Transfected cell lysate, Transfected cell line samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Alpha-crystallin B chain.
IgG1
Mouse
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Expected |
Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
Rat | Expected | Expected | Tested | Expected | Expected |
African green monkey | Expected | Expected | Tested | Tested | Expected |
Cow | Predicted | Predicted | Predicted | Predicted | Predicted |
Cynomolgus monkey | Predicted | Predicted | Predicted | Predicted | Predicted |
Orangutan | Predicted | Predicted | Predicted | Predicted | Predicted |
Pig | Predicted | Predicted | Predicted | Predicted | Predicted |
Rabbit | Predicted | Predicted | Predicted | Predicted | Predicted |
Sheep | Predicted | Predicted | Predicted | Predicted | Predicted |
Transfected cell line | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
Transfected cell lysate | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species African green monkey | Dilution info 10 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Sheep, Rabbit, Cow, Pig, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate, Transfected cell line | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Sheep, Rabbit, Cow, Pig, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate, Transfected cell line | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500 - 1/1000 | Notes - |
Species African green monkey | Dilution info 1/500 - 1/1000 | Notes - |
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
Species Transfected cell lysate | Dilution info 1/500 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Sheep, Rabbit, Cow, Pig, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info 1/50 - 1/100 | Notes - |
Species Human | Dilution info 1/50 - 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Sheep, Rabbit, Cow, Pig, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate, Transfected cell line | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Sheep, Rabbit, Cow, Pig, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
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May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).
CRYA2, HSPB5, HSPB5, CRYA2, CRYAB, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Heat shock protein family B member 5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, HspB5
Mouse Monoclonal Alpha B Crystallin antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, African green monkey, Transfected cell lysate, Transfected cell line samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Alpha-crystallin B chain.
IgG1
Mouse
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
6D11
Affinity purification Protein A/G
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Alpha B Crystallin also known as CRYAB or alpha B crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is expressed in various tissues including the eye lens heart skeletal muscles and the brain. Alpha B Crystallin functions mechanically as a chaperone helping to prevent the aggregation of unfolded proteins by stabilizing them. This protein plays an important role in maintaining proper cellular function especially under stress conditions.
Alpha B crystallin performs as part of the larger small heat shock protein family which contributes to the cellular defense system. It acts primarily as a chaperone binding to denatured proteins to protect the cells from damage during environmental stresses such as heat and oxidative stress. Alpha B crystallin often forms oligomeric complexes which increases its protective properties. This protein exhibits significant anti-apoptotic functions by interacting with various apoptosis-regulating factors contributing to cellular survival.
Alpha B crystallin integrates into the cellular stress response and apoptosis pathways. It involves the PI3K/Akt signaling pathway known for regulating cell survival and preventing programmed cell death. Alpha B crystallin also interacts with other proteins such as Bcl-2 further enhancing its role in anti-apoptotic activities. Its contribution to these pathways highlights its protective function during cellular stress situations.
Alpha B crystallin is associated with cataracts and dilated cardiomyopathy. It plays a significant role in maintaining lens transparency and mutations or dysfunctions in alpha B crystallin can lead to cataract development. Similarly in cardiac tissue abnormal alpha B crystallin expression links to dilated cardiomyopathy. The protein is also known to associate with other structural proteins like desmin where disruptions may contribute to muscle-related diseases.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722) at 1/500 dilution
Lane 1: pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Lane 2: pCMV6-ENTRY Alpha B Crystallin-transfected HEK-293T whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 20 kDa
COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for Alpha B Crystallin (green) using ab230722 at 1/50 dilution in ICC/IF.
Formalin-fixed, paraffin-embedded human heart tissue stained for Alpha B Cristallin using ab230722 at 10μg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human thyroid carcinoma tissue stained for Alpha B Cristallin using ab230722 at 1/50 dilution in immunohistochemical analysis.
KAT7 / Hbo1 / MYST2 was immunoprecipitated from 1mg of DDK tagged Alpha B Crystallin overexpression lysate at a 1:5 dilution with HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2μg ab230722..
Lane 1: IP without ab230722 in DDK tagged Alpha B Crystallin overexpression lysate at a 1:5 dilution with HEK-293T whole cell lysate (negative control)
Lane 2: ab230722 IP in DDK tagged Alpha B Crystallin overexpression lysate at a 1:5 dilution with HEK-293T whole cell lysate.
All lanes: Immunoprecipitation - Anti-Alpha B Crystallin antibody [6D11] (ab230722)
Developed using the ECL technique.
Predicted band size: 20 kDa
HT-29 (human colorectal adenocarcinoma cell line) cells stained for Alpha B Crystallin (green) using ab230722 at 1/50 dilution in ICC/IF.
All lanes: Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722) at 1/500 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg
Lane 3: HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 35 µg
Lane 4: A549 (human lung carcinoma cell line) whole cell lysate at 35 µg
Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg
Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg
Lane 7: MDCK (canine kidney cell line) whole cell lysate at 35 µg
Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg
Lane 9: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 20 kDa
Intracellular flow cytometric analysis of Alpha B Crystallin overexpression plasmid-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line labeling Alpha B Crystallin with 230722 at 1/100 dilution (red) compared withan empty vector control plasmid-transfected HEK-293T cells (blue).
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