AB230722

Anti-Alpha B Crystallin antibody [6D11]

Name: Anti-Alpha B Crystallin antibody [6D11] (ab230722) | Abcam
Brand: Abcam Limited
SKU: AB230722
Price: 605 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal Alpha B Crystallin antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, African green monkey, Transfected cell lysate, Transfected cell line samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CRYAB.

View Alternative Names

CRYA2, HSPB5, CRYAB, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Heat shock protein family B member 5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, HspB5

8 Images

Flow Cytometry (Intracellular) - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Intracellular flow cytometric analysis of Alpha B Crystallin overexpression plasmid-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line labeling Alpha B Crystallin with 230722 at 1/100 dilution (red) compared withan empty vector control plasmid-transfected HEK-293T cells (blue).

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

HT-29 (human colorectal adenocarcinoma cell line) cells stained for Alpha B Crystallin (green) using ab230722 at 1/50 dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Formalin-fixed, paraffin-embedded human heart tissue stained for Alpha B Cristallin using ab230722 at 10μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Formalin-fixed, paraffin-embedded human thyroid carcinoma tissue stained for Alpha B Cristallin using ab230722 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Immunoprecipitation - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

KAT7 / Hbo1 / MYST2 was immunoprecipitated from 1mg of DDK tagged Alpha B Crystallin overexpression lysate at a 1 : 5 dilution with HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2μg ab230722..

Lane 1 : IP without ab230722 in DDK tagged Alpha B Crystallin overexpression lysate at a 1 : 5 dilution with HEK-293T whole cell lysate (negative control)

Lane 2 : ab230722 IP in DDK tagged Alpha B Crystallin overexpression lysate at a 1 : 5 dilution with HEK-293T whole cell lysate.

All lanes:

Immunoprecipitation - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

Predicted band size: 20 kDa

true

Supplier Data

Western blot - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

All lanes:

Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

Lane 3:

HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) whole cell lysate at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

Lane 7:

MDCK (canine kidney cell line) whole cell lysate at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg

Predicted band size: 20 kDa

true

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for Alpha B Crystallin (green) using ab230722 at 1/50 dilution in ICC/IF.

Supplier Data

Western blot - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

All lanes:

Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722) at 1/500 dilution

Lane 1:

pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY Alpha B Crystallin-transfected HEK-293T whole cell lysate at 5 µg

Predicted band size: 20 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6D11

Isotype

IgG1

Carrier free

Reacts with

Rat, Human, African green monkey

Applications

ICC/IF, Flow Cyt (Intra), IP, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CRYAB.

P02511

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha B Crystallin also known as CRYAB or alpha B crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is expressed in various tissues including the eye lens heart skeletal muscles and the brain. Alpha B Crystallin functions mechanically as a chaperone helping to prevent the aggregation of unfolded proteins by stabilizing them. This protein plays an important role in maintaining proper cellular function especially under stress conditions.

Biological function summary

Alpha B crystallin performs as part of the larger small heat shock protein family which contributes to the cellular defense system. It acts primarily as a chaperone binding to denatured proteins to protect the cells from damage during environmental stresses such as heat and oxidative stress. Alpha B crystallin often forms oligomeric complexes which increases its protective properties. This protein exhibits significant anti-apoptotic functions by interacting with various apoptosis-regulating factors contributing to cellular survival.

Pathways

Alpha B crystallin integrates into the cellular stress response and apoptosis pathways. It involves the PI3K/Akt signaling pathway known for regulating cell survival and preventing programmed cell death. Alpha B crystallin also interacts with other proteins such as Bcl-2 further enhancing its role in anti-apoptotic activities. Its contribution to these pathways highlights its protective function during cellular stress situations.

Alpha B crystallin is associated with cataracts and dilated cardiomyopathy. It plays a significant role in maintaining lens transparency and mutations or dysfunctions in alpha B crystallin can lead to cataract development. Similarly in cardiac tissue abnormal alpha B crystallin expression links to dilated cardiomyopathy. The protein is also known to associate with other structural proteins like desmin where disruptions may contribute to muscle-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).

See full target information CRYAB

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Science progress 108:368504251343177 PubMed40390653

2025

αB-crystallin enhances radioresistance in basal-like breast cancer cells by suppressing caspase-3 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Yuan,Xubaihe Zhang,Ying Xu,Yangyang Ge,Anqing Wu

Frontiers in cell and developmental biology 9:802457 PubMed34977041

2021

A Single-Cell Transcriptome Atlas of the Human Retinal Pigment Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Zongren Xu,Xingyun Liao,Na Li,Hongxiu Zhou,Hong Li,Qi Zhang,Ke Hu,Peizeng Yang,Shengping Hou

In vitro cellular & developmental biology. Animal 57:359-371 PubMed33559028

2021

Copper toxicity of inflection point in human intestinal cell line Caco-2 dissected: influence of temporal expression patterns.

Applications

Unspecified application

Species

Unspecified reactive species

Joanne Keenan,Paula Meleady,Charles O'Doherty,Michael Henry,Martin Clynes,Karina Horgan,Richard Murphy,Finbarr O'Sullivan

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 31:4088-4103 PubMed28559441

2017

APMAP interacts with lysyl oxidase-like proteins, and disruption of leads to beneficial visceral adipose tissue expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Ariane R Pessentheiner,Katharina Huber,Helmut J Pelzmann,Andreas Prokesch,Franz P W Radner,Heimo Wolinski,Josefine Lindroos-Christensen,Gerald Hoefler,Thomas Rülicke,Ruth Birner-Gruenberger,Martin Bilban,Juliane G Bogner-Strauss

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha B Crystallin antibody [6D11]

Flow Cytometry (Intracellular) - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Immunoprecipitation - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Western blot - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Western blot - Anti-Alpha B Crystallin antibody [6D11] (AB230722)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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