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Mouse Monoclonal Alpha B Crystallin antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, African green monkey, Transfected cell lysate, Transfected cell line samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Alpha-crystallin B chain.

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Images

Western blot - Anti-Alpha B Crystallin antibody [6D11] (AB230722), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (AB230722), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (AB230722), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (AB230722), expandable thumbnail
  • Immunoprecipitation - Anti-Alpha B Crystallin antibody [6D11] (AB230722), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human Alpha-crystallin B chain. Database link P02511

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Expected
Mouse
Predicted
Predicted
Predicted
Predicted
Predicted
Rat
Expected
Expected
Tested
Expected
Expected
African green monkey
Expected
Expected
Tested
Tested
Expected
Cow
Predicted
Predicted
Predicted
Predicted
Predicted
Cynomolgus monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Orangutan
Predicted
Predicted
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Predicted
Transfected cell line
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Transfected cell lysate
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

-

Notes

-

Species

African green monkey

Dilution info

10 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Sheep, Rabbit, Cow, Pig, Cynomolgus monkey, Orangutan

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate, Transfected cell line

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Sheep, Rabbit, Cow, Pig, Cynomolgus monkey, Orangutan

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate, Transfected cell line

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/500 - 1/1000

Notes

-

Species

African green monkey

Dilution info

1/500 - 1/1000

Notes

-

Species

Human

Dilution info

1/500 - 1/1000

Notes

-

Species

Transfected cell lysate

Dilution info

1/500 - 1/1000

Notes

-

Predicted
Predicted

Species

Mouse, Sheep, Rabbit, Cow, Pig, Cynomolgus monkey, Orangutan

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell line

Dilution info

-

Notes

-

Tested
Tested

Species

African green monkey

Dilution info

1/50 - 1/100

Notes

-

Species

Human

Dilution info

1/50 - 1/100

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Sheep, Rabbit, Cow, Pig, Cynomolgus monkey, Orangutan

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate, Transfected cell line

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Rat, African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Sheep, Rabbit, Cow, Pig, Cynomolgus monkey, Orangutan

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate

Dilution info

-

Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).

Alternative names

Recommended products

Mouse Monoclonal Alpha B Crystallin antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, African green monkey, Transfected cell lysate, Transfected cell line samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Alpha-crystallin B chain.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human Alpha-crystallin B chain. Database link P02511
Clone number

6D11

Purification technique

Affinity purification Protein A/G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Alpha B Crystallin also known as CRYAB or alpha B crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is expressed in various tissues including the eye lens heart skeletal muscles and the brain. Alpha B Crystallin functions mechanically as a chaperone helping to prevent the aggregation of unfolded proteins by stabilizing them. This protein plays an important role in maintaining proper cellular function especially under stress conditions.

Biological function summary

Alpha B crystallin performs as part of the larger small heat shock protein family which contributes to the cellular defense system. It acts primarily as a chaperone binding to denatured proteins to protect the cells from damage during environmental stresses such as heat and oxidative stress. Alpha B crystallin often forms oligomeric complexes which increases its protective properties. This protein exhibits significant anti-apoptotic functions by interacting with various apoptosis-regulating factors contributing to cellular survival.

Pathways

Alpha B crystallin integrates into the cellular stress response and apoptosis pathways. It involves the PI3K/Akt signaling pathway known for regulating cell survival and preventing programmed cell death. Alpha B crystallin also interacts with other proteins such as Bcl-2 further enhancing its role in anti-apoptotic activities. Its contribution to these pathways highlights its protective function during cellular stress situations.

Associated diseases and disorders

Alpha B crystallin is associated with cataracts and dilated cardiomyopathy. It plays a significant role in maintaining lens transparency and mutations or dysfunctions in alpha B crystallin can lead to cataract development. Similarly in cardiac tissue abnormal alpha B crystallin expression links to dilated cardiomyopathy. The protein is also known to associate with other structural proteins like desmin where disruptions may contribute to muscle-related diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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8 product images

  • Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722), expandable thumbnail

    Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

    All lanes: Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722) at 1/500 dilution

    Lane 1: pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

    Lane 2: pCMV6-ENTRY Alpha B Crystallin-transfected HEK-293T whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 20 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (ab230722), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

    COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for Alpha B Crystallin (green) using ab230722 at 1/50 dilution in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (ab230722), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

    Formalin-fixed, paraffin-embedded human heart tissue stained for Alpha B Cristallin using ab230722 at 10μg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (ab230722), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

    Formalin-fixed, paraffin-embedded human thyroid carcinoma tissue stained for Alpha B Cristallin using ab230722 at 1/50 dilution in immunohistochemical analysis.

  • Immunoprecipitation - Anti-Alpha B Crystallin antibody [6D11] (ab230722), expandable thumbnail

    Immunoprecipitation - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

    KAT7 / Hbo1 / MYST2 was immunoprecipitated from 1mg of DDK tagged Alpha B Crystallin overexpression lysate at a 1:5 dilution with HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2μg ab230722..

    Lane 1: IP without ab230722 in DDK tagged Alpha B Crystallin overexpression lysate at a 1:5 dilution with HEK-293T whole cell lysate (negative control)

    Lane 2: ab230722 IP in DDK tagged Alpha B Crystallin overexpression lysate at a 1:5 dilution with HEK-293T whole cell lysate.

    All lanes: Immunoprecipitation - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

    Developed using the ECL technique.

    Predicted band size: 20 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (ab230722), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

    HT-29 (human colorectal adenocarcinoma cell line) cells stained for Alpha B Crystallin (green) using ab230722 at 1/50 dilution in ICC/IF.

  • Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722), expandable thumbnail

    Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

    All lanes: Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722) at 1/500 dilution

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

    Lane 3: HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 35 µg

    Lane 4: A549 (human lung carcinoma cell line) whole cell lysate at 35 µg

    Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg

    Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

    Lane 7: MDCK (canine kidney cell line) whole cell lysate at 35 µg

    Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg

    Lane 9: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 20 kDa

  • Flow Cytometry (Intracellular) - Anti-Alpha B Crystallin antibody [6D11] (ab230722), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

    Intracellular flow cytometric analysis of Alpha B Crystallin overexpression plasmid-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line labeling Alpha B Crystallin with 230722 at 1/100 dilution (red) compared withan empty vector control plasmid-transfected HEK-293T cells (blue).

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Product protocols

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