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AB13497

Anti-Alpha B Crystallin antibody

5

(4 Reviews)

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(25 Publications)

Rabbit Polyclonal Alpha B Crystallin antibody. Suitable for ICC, IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Pig, Chicken, Human, Cow samples. Cited in 25 publications. Immunogen corresponding to Synthetic Peptide within Human CRYAB aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CRYA2, HSPB5, CRYAB, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Heat shock protein family B member 5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, HspB5

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody (AB13497)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody (AB13497)

Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked (at 42°C for 1h) Cervical cancer cell line (HeLa) for 20min at RT, labelling Alpha B Crystallin with ab13497 at 1/120 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (Green) at 1/200 dilution for 2 hours at RT. Counterstain : DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT. Image showing actin filament bundles, nuclear splicing speckles, and Exosome staining. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody (green). (C) Composite image.

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody (AB13497)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody (AB13497)

Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked (at 42°C for 1h) Cervical cancer cell line (HeLa) for 20min at RT, labelling Alpha B Crystallin with ab13497 at 1/120 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (APC) (Red) at 1/200 dilution for 2 hours at RT. Counterstain : DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT. Image showing actin filament bundles, nuclear splicing speckles, and Exosome staining. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody (Red). (C) Composite image.

Western blot - Anti-Alpha B Crystallin antibody (AB13497)
  • WB

Supplier Data

Western blot - Anti-Alpha B Crystallin antibody (AB13497)

Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497).

Lane 1 : Molecular Weight Ladder (MW).

Lane 2 : Rat Brain cell lysates.

Load : 15 μg. Block : 5% Skim Milk in 1X TBST. Primary Antibody : Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497) at 1/1000 dilution for 60 min at RT. Secondary Antibody : Goat Anti-Rabbit IgG : HRP at 1/1000 dilution for 60 min at RT. Color Development : ECL solution for 6 min in RT. Predicted/Observed Size : ~22 kDa.

All lanes:

Western blot - Anti-Alpha B Crystallin antibody (ab13497)

Predicted band size: 20 kDa

true

Western blot - Anti-Alpha B Crystallin antibody (AB13497)
  • WB

Unknown

Western blot - Anti-Alpha B Crystallin antibody (AB13497)

All lanes:

Western blot - Anti-Alpha B Crystallin antibody (ab13497) at 1/1000 dilution

All lanes:

Human spinal cord tissue lysate - total protein (<a href='/en-us/products/unavailable/human-spinal-cord-tissue-lysate-total-protein-ab29188'>ab29188</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 20 kDa

Observed band size: 22 kDa

false

Western blot - Anti-Alpha B Crystallin antibody (AB13497)
  • WB

Supplier Data

Western blot - Anti-Alpha B Crystallin antibody (AB13497)

Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497).

Lane 1 : Molecular Weight Ladder (MW).

Lane 2 : A431 cell lysates.

Lane 3 : HCT116 cell lysates.

Lane 4 : HeLa cell lysates.

Lane 5 : HepG2 cell lysates.

Lane 6 : HEK293 cell lysates.

Lane 7 : HUVEC cell lysates.

Lane 8 : Jurkat cell lysates.

Lane 9 : MCF7 cell lysates.

Lane 10 : PC3 cell lysates.

Lane 11 : T98G cell lysates.

Load : 15 μg. Block : 5% Skim Milk in 1X TBST. Primary Antibody : Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497) at 1/1000 dilution for 60 min at RT. Secondary Antibody : Goat Anti-Rabbit IgG : HRP at 1/1000 dilution for 60 min at RT. Color Development : ECL solution for 6 min in RT. Predicted/Observed Size : ~22 kDa.

All lanes:

Western blot - Anti-Alpha B Crystallin antibody (ab13497)

Predicted band size: 20 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Chicken, Cow, Pig, Mouse, Rat

Applications

IHC-Fr, ICC/IF, ICC, IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human CRYAB aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P02511

Specificity

Does not cross-react with αA-crystallin.

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Reactivity data

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Product details

For maximum product recovery, after thawing, centrifuge the product vial before removing cap
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49.1% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha B Crystallin also known as CRYAB or alpha B crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is expressed in various tissues including the eye lens heart skeletal muscles and the brain. Alpha B Crystallin functions mechanically as a chaperone helping to prevent the aggregation of unfolded proteins by stabilizing them. This protein plays an important role in maintaining proper cellular function especially under stress conditions.
Biological function summary

Alpha B crystallin performs as part of the larger small heat shock protein family which contributes to the cellular defense system. It acts primarily as a chaperone binding to denatured proteins to protect the cells from damage during environmental stresses such as heat and oxidative stress. Alpha B crystallin often forms oligomeric complexes which increases its protective properties. This protein exhibits significant anti-apoptotic functions by interacting with various apoptosis-regulating factors contributing to cellular survival.

Pathways

Alpha B crystallin integrates into the cellular stress response and apoptosis pathways. It involves the PI3K/Akt signaling pathway known for regulating cell survival and preventing programmed cell death. Alpha B crystallin also interacts with other proteins such as Bcl-2 further enhancing its role in anti-apoptotic activities. Its contribution to these pathways highlights its protective function during cellular stress situations.

Alpha B crystallin is associated with cataracts and dilated cardiomyopathy. It plays a significant role in maintaining lens transparency and mutations or dysfunctions in alpha B crystallin can lead to cataract development. Similarly in cardiac tissue abnormal alpha B crystallin expression links to dilated cardiomyopathy. The protein is also known to associate with other structural proteins like desmin where disruptions may contribute to muscle-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).
See full target information CRYAB
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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38474037

2024

Protein Kinase D Plays a Crucial Role in Maintaining Cardiac Homeostasis by Regulating Post-Translational Modifications of Myofilament Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Herwig,Merima Begovic,Heidi Budde,Simin Delalat,Saltanat Zhazykbayeva,Marcel Sieme,Luca Schneider,Kornelia Jaquet,Andreas Mügge,Ibrahim Akin,Ibrahim El-Battrawy,Jens Fielitz,Nazha Hamdani

HGG advances 5:100274 PubMed38358893

2024

Clinical, immunohistochemical, and genetic characterization of splice-altering biallelic DES variants: Therapeutic implications.

Applications

Unspecified application

Species

Unspecified reactive species

Janelle Geist Hauserman,Chamindra G Laverty,Sandra Donkervoort,Ying Hu,Sarah Silverstein,Sarah B Neuhaus,Dimah Saade,Gabrielle Vaughn,Denise Malicki,Rupleen Kaur,Yuesheng Li,Yan Luo,Poching Liu,Patrick Burr,A Reghan Foley,Payam Mohassel,Carsten G Bönnemann

Genetics 225: PubMed37713608

2023

Identification of CryAB as a target of NUAK kinase activity in Drosophila muscle tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwei Zhao,David Brooks,Yungui Guo,Erika R Geisbrecht

Frontiers in cardiovascular medicine 10:1157398 PubMed37363100

2023

Oxidative stress and inflammation distinctly drive molecular mechanisms of diastolic dysfunction and remodeling in female and male heart failure with preserved ejection fraction rats.

Applications

Unspecified application

Species

Unspecified reactive species

Saltanat Zhazykbayeva,Roua Hassoun,Melissa Herwig,Heidi Budde,Árpád Kovács,Hans Georg Mannherz,Ibrahim El-Battrawy,Attila Tóth,Wolfgang E Schmidt,Andreas Mügge,Nazha Hamdani

Allergologia et immunopathologia 50:23-29 PubMed36086960

2022

CRYAB reduces cigarette smoke-induced inflammation, apoptosis, and oxidative stress by retarding PI3K/Akt and NF-κB signaling pathways in human bronchial epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shiliang Xie,Xiaofeng Wang

Experimental eye research 205:108472 PubMed33516765

2021

A tissue-engineered human trabecular meshwork hydrogel for advanced glaucoma disease modeling.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Li,Tyler Bagué,Alexander Kirschner,Ana N Strat,Haven Roberts,Robert W Weisenthal,Alison E Patteson,Nasim Annabi,W Daniel Stamer,Preethi S Ganapathy,Samuel Herberg

Journal of the American Society of Nephrology : JASN 31:118-138 PubMed31818909

2019

Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Sébastien J Dumas,Elda Meta,Mila Borri,Jermaine Goveia,Katerina Rohlenova,Nadine V Conchinha,Kim Falkenberg,Laure-Anne Teuwen,Laura de Rooij,Joanna Kalucka,Rongyuan Chen,Shawez Khan,Federico Taverna,Weisi Lu,Magdalena Parys,Carla De Legher,Stefan Vinckier,Tobias K Karakach,Luc Schoonjans,Lin Lin,Lars Bolund,Mieke Dewerchin,Guy Eelen,Ton J Rabelink,Xuri Li,Yonglun Luo,Peter Carmeliet

Cell stress & chaperones 23:1041-1054 PubMed29797237

2018

Effects of 10 weeks of regular running exercise with and without parallel PDTC treatment on expression of genes encoding sarcomere-associated proteins in murine skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Angelika Schmitt,Anne-Lena Haug,Franziska Schlegel,Annunziata Fragasso,Barbara Munz

Frontiers in physiology 9:505 PubMed29867551

2018

BYD Ameliorates Oxidative Stress-Induced Myocardial Apoptosis in Heart Failure Post-Acute Myocardial Infarction via the P38 MAPK-CRYAB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Chun Li,Hui Meng,Dongqing Guo,Qian Zhang,Wenji Lu,Qixin Wang,Yong Wang,Pengfei Tu

JCI insight 3: PubMed29515024

2018

Identification of periplakin as a major regulator of lung injury and repair in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Valérie Besnard,Rania Dagher,Tania Madjer,Audrey Joannes,Madeleine Jaillet,Martin Kolb,Philippe Bonniaud,Lynne A Murray,Matthew A Sleeman,Bruno Crestani
View all publications
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