Name: Anti-Alpha B Crystallin antibody [EPR2752]
SKU: ab76467
Rating: 5 (2 reviews)

Rabbit Recombinant Monoclonal Alpha B Crystallin antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Rat samples. Cited in 8 publications.

Images

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	Flow Cyt	WB	ICC/IF
Human	Tested	Tested	Not recommended	Tested	Tested
Rat	Expected	Expected	Not recommended	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/1000 - 1/2000	Notes -
Species Human	Dilution info 1/1000 - 1/2000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information CRYAB

Function

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).

Alternative names

CRYA2, HSPB5, CRYAB, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Heat shock protein family B member 5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, HspB5

Recommended products

Rabbit Recombinant Monoclonal Alpha B Crystallin antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Rat samples. Cited in 8 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR2752
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Alpha B Crystallin also known as CRYAB or alpha B crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is expressed in various tissues including the eye lens heart skeletal muscles and the brain. Alpha B Crystallin functions mechanically as a chaperone helping to prevent the aggregation of unfolded proteins by stabilizing them. This protein plays an important role in maintaining proper cellular function especially under stress conditions.

Biological function summary

Alpha B crystallin performs as part of the larger small heat shock protein family which contributes to the cellular defense system. It acts primarily as a chaperone binding to denatured proteins to protect the cells from damage during environmental stresses such as heat and oxidative stress. Alpha B crystallin often forms oligomeric complexes which increases its protective properties. This protein exhibits significant anti-apoptotic functions by interacting with various apoptosis-regulating factors contributing to cellular survival.

Pathways

Alpha B crystallin integrates into the cellular stress response and apoptosis pathways. It involves the PI3K/Akt signaling pathway known for regulating cell survival and preventing programmed cell death. Alpha B crystallin also interacts with other proteins such as Bcl-2 further enhancing its role in anti-apoptotic activities. Its contribution to these pathways highlights its protective function during cellular stress situations.

Associated diseases and disorders

Alpha B crystallin is associated with cataracts and dilated cardiomyopathy. It plays a significant role in maintaining lens transparency and mutations or dysfunctions in alpha B crystallin can lead to cataract development. Similarly in cardiac tissue abnormal alpha B crystallin expression links to dilated cardiomyopathy. The protein is also known to associate with other structural proteins like desmin where disruptions may contribute to muscle-related diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution
All lanes: Rat eyeball lysate at 10 µg
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Alpha B Crystallin with purified ab76467 at 1/250. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Immunohistochemical staining of paraffin embedded human brain with purified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution
All lanes: Human fetal heart tissue lysate at 10 µg/mL
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Immunofluorescence staining of HeLa cells with purified ab76467 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
ab76467 (purified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Predicted band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Immunohistochemical staining of paraffin embedded human brain with unpurified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunoprecipitation - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
ab76467 (unpurified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Predicted band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Immunohistochemical staining of alpha B Crystallin in paraffin embedded human heart muscle with ab76467 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Lane 1: Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/5000 dilution
Lane 2: Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/20000 dilution
Lane 1: fetal heart lysate at 10 µg
Lane 2: rat eyeball lysate at 10 µg
Secondary
All lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)
Immunofluorescence staining of HeLa cells with unpurified ab76467 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.