AB76467

Anti-Alpha B Crystallin antibody [EPR2752]

Name: Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) | Abcam
Brand: Abcam Limited
SKU: AB76467
Price: 5050 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(15 Publications)

Rabbit Recombinant Monoclonal Alpha B Crystallin antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Rat samples. Cited in 15 publications.

View Alternative Names

CRYA2, HSPB5, CRYAB, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Heat shock protein family B member 5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, HspB5

11 Images

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunofluorescence staining of HeLa cells with purified ab76467 at a working dilution of 1 in 200 counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit used at a dilution of 1 in 200. The cells were fixed in 4% PFA.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunohistochemical staining of alpha B Crystallin in paraffin embedded human heart muscle with ab76467 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunohistochemical staining of paraffin embedded human brain with purified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunofluorescence staining of HeLa cells with unpurified ab76467 at a working dilution of 1 in 200 counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit used at a dilution of 1 in 200. The cells were fixed in 4% PFA.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Alpha B Crystallin with purified ab76467 at 1/250. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunohistochemical staining of paraffin embedded human brain with unpurified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0.

Unknown

Immunoprecipitation - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

ab76467 (unpurified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Predicted band size: 20 kDa

false

Lab

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution

All lanes:

Human fetal heart tissue lysate at 10 µg/mL

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 22 kDa

false

Lab

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

ab76467 (purified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Predicted band size: 20 kDa

false

Lab

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution

All lanes:

Rat eyeball lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 22 kDa

false

Unknown

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Lane 1:

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/5000 dilution

Lane 2:

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/20000 dilution

Lane 1:

fetal heart lysate at 10 µg

Lane 2:

rat eyeball lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Carrier free

Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Alpha B Crystallin antibody [EPR2752]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Alpha B Crystallin antibody [EPR2752]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2752

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IP, WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/250", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/250", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/250", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha B Crystallin also known as CRYAB or alpha B crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is expressed in various tissues including the eye lens heart skeletal muscles and the brain. Alpha B Crystallin functions mechanically as a chaperone helping to prevent the aggregation of unfolded proteins by stabilizing them. This protein plays an important role in maintaining proper cellular function especially under stress conditions.

Biological function summary

Alpha B crystallin performs as part of the larger small heat shock protein family which contributes to the cellular defense system. It acts primarily as a chaperone binding to denatured proteins to protect the cells from damage during environmental stresses such as heat and oxidative stress. Alpha B crystallin often forms oligomeric complexes which increases its protective properties. This protein exhibits significant anti-apoptotic functions by interacting with various apoptosis-regulating factors contributing to cellular survival.

Pathways

Alpha B crystallin integrates into the cellular stress response and apoptosis pathways. It involves the PI3K/Akt signaling pathway known for regulating cell survival and preventing programmed cell death. Alpha B crystallin also interacts with other proteins such as Bcl-2 further enhancing its role in anti-apoptotic activities. Its contribution to these pathways highlights its protective function during cellular stress situations.

Alpha B crystallin is associated with cataracts and dilated cardiomyopathy. It plays a significant role in maintaining lens transparency and mutations or dysfunctions in alpha B crystallin can lead to cataract development. Similarly in cardiac tissue abnormal alpha B crystallin expression links to dilated cardiomyopathy. The protein is also known to associate with other structural proteins like desmin where disruptions may contribute to muscle-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).

See full target information CRYAB

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Molecular and clinical oncology 23:72 PubMed40599718

2025

Nuclear factor IA-mediated transcriptional regulation of crystallin αB inhibits hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Jin,Pingping Hu,Yihe Dai,Wenchao Gu,Jiang Han,Haihan Song

CNS neuroscience & therapeutics 31:e70398 PubMed40296303

2025

Effects of the Small-Molecule ISRIB on the Rapid and Efficient Myelination of Oligodendrocytes in Human Stem Cell-Derived Cerebral Organoids in Patients With Leukoencephalopathy With Vanishing White Matter.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yan,Jiong Deng,Jie Zhang,Kai Gao,Huan Yi,Junjiao Zhang,Fan Zhang,Jingmin Wang,Yuwu Jiang,Ye Wu

Journal of extracellular biology 4:e70024 PubMed39844926

2025

Mesenchymal stem cell-derived exosomes mitigate amyloid β-induced retinal toxicity: Insights from rat model and cellular studies.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Qarawani,Efrat Naaman,Rony Ben-Zvi Elimelech,Michal Harel,Shahaf Sigal-Dror,Tali Ben-Zur,Tamar Ziv,Daniel Offen,Shiri Zayit-Soudry

Frontiers in cell and developmental biology 12:1526943 PubMed39834393

2025

Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Yufan Liu,Zuowei Wang,Tianju Ma,Yi Gao,Wenqian Chen,Zi Ye,Zhaohui Li

iScience 27:109510 PubMed38660406

2024

The activation of LBH-CRYAB signaling promotes cardiac protection against I/R injury by inhibiting apoptosis and ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Anbiao Wu,Chongbin Zhong,Xudong Song,Wen Yuan,Mintian Tang,Tao Shu,Houda Huang,Pingzhen Yang,Qicai Liu

iScience 27:109397 PubMed38510120

2024

Deciphering the spatiotemporal transcriptional and chromatin accessibility of human retinal organoid development at the single-cell level.

Applications

Unspecified application

Species

Unspecified reactive species

Birthe Dorgau,Joseph Collin,Agata Rozanska,Veronika Boczonadi,Marina Moya-Molina,Adrienne Unsworth,Rafiqul Hussain,Jonathan Coxhead,Tamil Dhanaseelan,Lyle Armstrong,Rachel Queen,Majlinda Lako

Cell stress & chaperones 28:787-799 PubMed37584866

2023

Regulation of rat HspB5/alphaB-Crystallin by microRNAs miR-101a-3p, miR-140-5p, miR-330-5p, and miR-376b-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Britta Bartelt-Kirbach,Nikola Golenhofen

Frontiers in cardiovascular medicine 10:1223244 PubMed37435054

2023

A novel αB-crystallin R123W variant drives hypertrophic cardiomyopathy by promoting maladaptive calcium-dependent signal transduction.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Chou,Gregory L Martin,Gayani Perera,Junya Awata,Amy Larson,Robert Blanton,Michael T Chin

CNS neuroscience & therapeutics 29:1049-1066 PubMed36650674

2023

Human-induced pluripotent stem cell-derived cerebral organoid of leukoencephalopathy with vanishing white matter.

Applications

Unspecified application

Species

Unspecified reactive species

Jiong Deng,Jie Zhang,Kai Gao,Ling Zhou,Yuwu Jiang,Jingmin Wang,Ye Wu

Stem cells translational medicine 11:159-177 PubMed35298655

2022

Human Retinal Organoids Provide a Suitable Tool for Toxicological Investigations: A Comprehensive Validation Using Drugs and Compounds Affecting the Retina.

Applications

Unspecified application

Species

Unspecified reactive species

Birthe Dorgau,Maria Georgiou,Alexander Chaudhary,Marina Moya-Molina,Joseph Collin,Rachel Queen,Gerrit Hilgen,Tracey Davey,Philip Hewitt,Michael Schmitt,Stefan Kustermann,Francois Pognan,David H Steel,Evelyne Sernagor,Lyle Armstrong,Majlinda Lako

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha B Crystallin antibody [EPR2752]

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Immunoprecipitation - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (AB76467)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise