AB247476

Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Alpha B Crystallin antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

CRYA2, HSPB5, CRYAB, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Heat shock protein family B member 5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, HspB5

11 Images

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

This data was developed using ab76467, the same antibody clone in a different buffer formulation.

Immunofluorescence staining of HeLa cells with purified ab76467 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

This data was developed using ab76467, the same antibody clone in a different buffer formulation.

Immunofluorescence staining of HeLa cells with unpurified ab76467 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

This data was developed using ab76467, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin embedded human brain with purified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

This data was developed using ab76467, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of alpha B Crystallin in paraffin embedded human heart muscle with ab76467 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

This data was developed using ab76467, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Alpha B Crystallin with purified ab76467 at 1/250. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

This data was developed using ab76467, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin embedded human brain with unpurified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Unknown

Immunoprecipitation - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

This data was developed using ab76467, the same antibody clone in a different buffer formulation.

ab76467 (unpurified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-Alpha B Crystallin antibody [EPR2752] (<a href='/en-us/products/primary-antibodies/alpha-b-crystallin-antibody-epr2752-ab76467'>ab76467</a>)

Predicted band size: 20 kDa

false

Lab

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

This data was developed using ab76467, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (<a href='/en-us/products/primary-antibodies/alpha-b-crystallin-antibody-epr2752-ab76467'>ab76467</a>) at 1/1000 dilution

All lanes:

Human fetal heart tissue lysate at 10 µg/mL

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 22 kDa

false

Lab

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

This data was developed using ab76467, the same antibody clone in a different buffer formulation.

ab76467 (purified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (<a href='/en-us/products/primary-antibodies/alpha-b-crystallin-antibody-epr2752-ab76467'>ab76467</a>)

Predicted band size: 20 kDa

false

Lab

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

This data was developed using ab76467, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (<a href='/en-us/products/primary-antibodies/alpha-b-crystallin-antibody-epr2752-ab76467'>ab76467</a>) at 1/1000 dilution

All lanes:

Rat eyeball lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 22 kDa

false

Unknown

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

This data was developed using ab76467, the same antibody clone in a different buffer formulation.

Lane 1:

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476) at 1/5000 dilution

Lane 2:

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476) at 1/20000 dilution

Lane 1:

Fetal heart tissue lysate at 10 µg

Lane 2:

Rat eyeball tissue lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Unconjugated

Anti-Alpha B Crystallin antibody [EPR2752]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Alpha B Crystallin antibody [EPR2752]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Alpha B Crystallin antibody [EPR2752]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2752

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, ICC/IF, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab247476 is the carrier-free version of ab76467.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha B Crystallin also known as CRYAB or alpha B crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is expressed in various tissues including the eye lens heart skeletal muscles and the brain. Alpha B Crystallin functions mechanically as a chaperone helping to prevent the aggregation of unfolded proteins by stabilizing them. This protein plays an important role in maintaining proper cellular function especially under stress conditions.

Biological function summary

Alpha B crystallin performs as part of the larger small heat shock protein family which contributes to the cellular defense system. It acts primarily as a chaperone binding to denatured proteins to protect the cells from damage during environmental stresses such as heat and oxidative stress. Alpha B crystallin often forms oligomeric complexes which increases its protective properties. This protein exhibits significant anti-apoptotic functions by interacting with various apoptosis-regulating factors contributing to cellular survival.

Pathways

Alpha B crystallin integrates into the cellular stress response and apoptosis pathways. It involves the PI3K/Akt signaling pathway known for regulating cell survival and preventing programmed cell death. Alpha B crystallin also interacts with other proteins such as Bcl-2 further enhancing its role in anti-apoptotic activities. Its contribution to these pathways highlights its protective function during cellular stress situations.

Alpha B crystallin is associated with cataracts and dilated cardiomyopathy. It plays a significant role in maintaining lens transparency and mutations or dysfunctions in alpha B crystallin can lead to cataract development. Similarly in cardiac tissue abnormal alpha B crystallin expression links to dilated cardiomyopathy. The protein is also known to associate with other structural proteins like desmin where disruptions may contribute to muscle-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).

See full target information CRYAB

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

Immunoprecipitation - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (AB247476)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise