Rabbit Polyclonal Alpha B Crystallin phospho S59 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CRYAB phospho S59 aa 50-100.
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- International journal of molecular sciences 23:2022Polo-Like Kinase 2 Plays an Essential Role in Cytoprotection against MG132-Induced Proteasome Inhibition via Phosphorylation of Serine 19 in HSPB5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuji Ueda,Moeka Nishihara,Yuuki Hioka,Ken-Ichi Yoshino,Soichiro Yamada,Minoru Yamanoue,Yasuhito ShiraiPubMed 36232565
- Antioxidants (Basel, Switzerland) 9:2020SkQ1 Suppresses the p38 MAPK Signaling Pathway Involved in Alzheimer's Disease-Like Pathology in OXYS Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia A Muraleva et. al.PubMed 32731533
- Redox biology 24:1011832019The early response of αB-crystallin to a single bout of aerobic exercise in mouse skeletal muscles depends upon fiber oxidative features.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvan Dimauro et. al.PubMed 30974319
- Laboratory investigation; a journal of technical m 99:1714-17272019Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenting Yu et. al.PubMed 30683901
- Frontiers in physiology 9:5052018BYD Ameliorates Oxidative Stress-Induced Myocardial Apoptosis in Heart Failure Post-Acute Myocardial Infarction via the P38 MAPK-CRYAB Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhang et. al.PubMed 29867551
- Journal of molecular and cellular cardiology 111:61-682017Exercise-responsive phosphoproteins in the heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongbo Guo et. al.PubMed 28826663
- Proceedings of the National Academy of Sciences of 114:E1745-E17542017Phosphorylation of αB-crystallin supports reactive astrogliosis in demyelination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHedwich F Kuipers et. al.PubMed 28196893
- Proteomics 15:508-5192014Quantitative phosphoproteomics of Alzheimer's disease reveals cross-talk between kinases and small heat shock proteins.Applications:WBReactive species:HumanEric B Dammer,Andrew K Lee,Duc M Duong,Marla Gearing,James J Lah,Allan I Levey,Nicholas T SeyfriedPubMed 25332170
- Journal of cellular physiology 229:1272-822014Cardioprotective role of P38 MAPK during myocardial infarction via parallel activation of α-crystallin B and Nrf2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArkadeep Mitra,Aramita Ray,Ritwik Datta,Shantanu Sengupta,Sagartirtha SarkarPubMed 24464634
- Proteomics 11:3369-792011Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJatin G Burniston,Jenna Kenyani,Jonathan M Wastling,Charles F Burant,Nathan R Qi,Lauren G Koch,Steven L BrittonPubMed 21751351
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 3% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CRYAB phospho S59 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P02511
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Cynomolgus monkey | Predicted |
| Hamster | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
| Sheep | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Hamster, Pig, Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).
Alternative names
CRYA2, HSPB5, CRYAB, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Heat shock protein family B member 5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, HspB5
Recommended products
Rabbit Polyclonal Alpha B Crystallin phospho S59 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CRYAB phospho S59 aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 3% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CRYAB phospho S59 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P02511
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha B Crystallin also known as CRYAB or alpha B crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is expressed in various tissues including the eye lens heart skeletal muscles and the brain. Alpha B Crystallin functions mechanically as a chaperone helping to prevent the aggregation of unfolded proteins by stabilizing them. This protein plays an important role in maintaining proper cellular function especially under stress conditions.
- Biological function summary
Alpha B crystallin performs as part of the larger small heat shock protein family which contributes to the cellular defense system. It acts primarily as a chaperone binding to denatured proteins to protect the cells from damage during environmental stresses such as heat and oxidative stress. Alpha B crystallin often forms oligomeric complexes which increases its protective properties. This protein exhibits significant anti-apoptotic functions by interacting with various apoptosis-regulating factors contributing to cellular survival.
- Pathways
Alpha B crystallin integrates into the cellular stress response and apoptosis pathways. It involves the PI3K/Akt signaling pathway known for regulating cell survival and preventing programmed cell death. Alpha B crystallin also interacts with other proteins such as Bcl-2 further enhancing its role in anti-apoptotic activities. Its contribution to these pathways highlights its protective function during cellular stress situations.
- Associated diseases and disorders
Alpha B crystallin is associated with cataracts and dilated cardiomyopathy. It plays a significant role in maintaining lens transparency and mutations or dysfunctions in alpha B crystallin can lead to cataract development. Similarly in cardiac tissue abnormal alpha B crystallin expression links to dilated cardiomyopathy. The protein is also known to associate with other structural proteins like desmin where disruptions may contribute to muscle-related diseases.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Alpha B Crystallin (phospho S59) antibody (ab5577)
ab5577 at 0.5μg/ml concentration staining approximately 20 KDa Crystallin Alpha B in lane 1) phospho-alpha-B crystallin (Ser59) and lane 2) non phosphorylated alpha-B crystallin in U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate.
All lanes: Western blot - Anti-Alpha B Crystallin (phospho S59) antibody (ab5577)
Predicted band size: 20 kDa
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