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AB99504

Anti-alpha Defensin 1 + 2 + 3 antibody

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(1 Publication)

Goat Polyclonal alpha Defensin 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DEFA1 aa 50 to C-terminus.

View Alternative Names

DEF1, DEFA2, MRS, DEFA1B, DEFA1, Neutrophil defensin 1, HNP-1, HP-1, HP1, DEF3, DEFA3, Neutrophil defensin 3, HNP-3, HP-3, HP3

1 Images
Western blot - Anti-alpha Defensin 1 + 2 + 3 antibody (AB99504)
  • WB

Unknown

Western blot - Anti-alpha Defensin 1 + 2 + 3 antibody (AB99504)

All lanes:

Western blot - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504) at 2 µg/mL

All lanes:

Human Spleen lysate in RIPA buffer at 35 µg

Predicted band size: 10 kDa

true

Exposure time: 1hr

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human DEFA1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P59665

Specificity

ab99504 is expected to equally recognize alpha Defensin 1 precursor and alpha Defensin 3 precursor.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4-6 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha defensin 1 2 and 3 often called human neutrophil peptides (HNP) 1 2 and 3 are small antimicrobial peptides with masses around 3-4 kDa. You commonly find them in neutrophils and Paneth cells of the small intestine. These peptides display broad-spectrum antimicrobial activity targeting bacteria fungi and some viruses. They insert themselves into microbial membranes causing membrane disruption and cell death. Despite their small size the alpha-defensins have significant effects on microbial populations promoting innate immunity.
Biological function summary

Alpha defensins participate in host defense by exerting antimicrobial action and immunomodulatory functions. They form part of the innate immune system and are integral to the antimicrobial complexes in neutrophils. The peptides interact with other immune components to modulate inflammation and enhance phagocytosis. By recruiting and activating immune cells alpha defensins help manage infections and influence the immune response.

Pathways

Alpha defensins are integral to the immune response and inflammation pathways. They interact with proteins like toll-like receptors (TLRs) to initiate signaling cascades that lead to cytokine production. Additionally the peptides participate in reactive oxygen species (ROS) generation which further aids microbial killing. Their role in these pathways allows immune coordination at the site of infection and contributes to pathogen clearance.

Alterations in alpha defensins' expression or function can link to inflammatory bowel disease (IBD) and cystic fibrosis. In IBD reduced alpha defensin levels can impair mucosal defense leading to increased bacterial translocation and inflammation. The peptides also connect to cystic fibrosis through their role in airway surface immobility and infection management. In these diseases alpha defensins interact with proteins like mucin to influence disease progression and patient outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Effector molecule of the innate immune system that acts via antibiotic-like properties against a broad array of infectious agents including bacteria, fungi, and viruses or by promoting the activation and maturation of some APCs (PubMed : 15616305, PubMed : 17142766, PubMed : 20220136, PubMed : 24236072). Interacts with the essential precursor of cell wall synthesis lipid II to inhibit bacterial cell wall synthesis (PubMed : 20214904). Inhibits adenovirus infection via inhibition of viral disassembly at the vertex region, thereby restricting the release of internal capsid protein pVI, which is required for endosomal membrane penetration during cell entry (PubMed : 18191790). In addition, interaction with adenovirus capsid leads to the redirection of viral particles to TLR4 thereby promoting a NLRP3-mediated inflammasome response and interleukin 1-beta (IL-1beta) release (PubMed : 35080426). Induces the production of proinflammatory cytokines including type I interferon (IFN) in plasmacytoid dendritic cells (pDCs) by triggering the degradation of NFKBIA and nuclear translocation of IRF1, both of which are required for activation of pDCs (PubMed : 27031443).
See full target information DEFA1

Additional targets

DEFA3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 28:1100-1116 PubMed30496485

2018

PD-linked CHCHD2 mutations impair CHCHD10 and MICOS complex leading to mitochondria dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhou,Dongrui Ma,Alfred Xuyang Sun,Hoang-Dai Tran,Dong-Liang Ma,Brijesh K Singh,Jin Zhou,Jinyan Zhang,Danlei Wang,Yi Zhao,Paul M Yen,Eyleen Goh,Eng-King Tan
View all publications
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Product promise

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