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AB62757

Anti-alpha Defensin 5 antibody [8C8]

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(5 Publications)

Mouse Monoclonal alpha Defensin 5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

DEF5, DEFA5, Defensin alpha 5, Defensin-5, HD5(20-94)

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 5 antibody [8C8] (AB62757)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 5 antibody [8C8] (AB62757)

IHC image of alpha Defensin 5 staining in human duodenum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab62757, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8C8

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Defensin 5 sometimes referred to as HD5 is a small antimicrobial peptide that exhibits an important role in the innate immune system. It has a molecular mass of approximately 3.5 kDa. Alpha Defensin 5 is mainly expressed in Paneth cells located in the small intestine and to a lesser extent in epithelial cells of other mucosal tissues. This peptide is known for its ability to disrupt microbial membranes providing a defense against a broad range of pathogens including bacteria fungi and some viruses.
Biological function summary

Alpha defensin 5 plays a significant role in maintaining the homeostasis of the gut microbiota. It is not typically part of a larger complex but it functions independently to modulate the intestinal environment. The peptide contributes to the protective barrier by influencing the microbial community composition aiding in the prevention of infections and the maintenance of gut health. Its mechanism includes direct bacterial lysis where it inserts into microbial cell membranes and forms pores leading to cell death.

Pathways

Alpha defensin 5 is integral within the innate immune response and antimicrobial peptide-mediated signaling pathways. It is closely tied to regulatory interactions with other proteins such as alpha defensins and beta defensins which similarly contribute to immune defense. While alpha defensin 5 acts independently it collaborates with these proteins to orchestrate a coordinated response against microbial infections enhancing the overall effectiveness of the host’s immune system.

Alpha defensin 5 has been related to inflammatory bowel diseases (IBD) and colorectal cancer. Altered expression levels of this peptide can disrupt the delicate balance of intestinal microflora potentially leading to chronic inflammation observed in IBD. Additionally its dysregulation might contribute to the mechanisms underlying colorectal cancer progression. In these conditions alpha defensin 5 interacts with other immune-modulating proteins affecting disease outcomes and suggesting its therapeutic potential as a target for disease intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Host-defense peptide that maintains sterility in the urogenital system (PubMed : 12021776, PubMed : 12660734, PubMed : 15616305, PubMed : 19589339, PubMed : 22359618, PubMed : 22573326, PubMed : 25354318, PubMed : 25782105, PubMed : 30808760). Has antimicrobial activity against a wide range of bacteria, including Gram-negative E.coli, P.aeruginosa and S.typhimurium, and Gram-positive E.aerogenes, S.aureus, B.cereus, E.faecium and L.monocytogenes (PubMed : 12021776, PubMed : 15616305, PubMed : 19589339, PubMed : 22359618, PubMed : 22573326, PubMed : 25354318, PubMed : 30808760). Confers resistance to intestinal infection by S.typhimurium (PubMed : 12660734). Exhibits antimicrobial activity against enteric commensal bacteria such as B.adolescentis, L.acidophilus, B.breve, L.fermentum, B.longum and S.thermophilus (PubMed : 25354318). Binds to bacterial membranes and causes membrane disintegration (PubMed : 25782105). Induces the secretion of the chemokine IL-8 by intestinal epithelial cells (PubMed : 19589339). Binds to B.antracis lef/lethal factor, a major virulence factor from B.anthracis, and neutralizes its enzymatic activity (PubMed : 22573326).. (Microbial infection) Acts as a target for S.flexneri infection by binding to the bacterium, possibly via bacterial surface proteins, and thereby augmenting infectivity via enhanced bacterial adhesion and invasion of epithelial cells and tissues.
See full target information DEFA5
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Mucosal immunology 12:491-502 PubMed30542108

2018

Age-of-diagnosis dependent ileal immune intensification and reduced alpha-defensin in older versus younger pediatric Crohn Disease patients despite already established dysbiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yael Haberman,Melanie Schirmer,Phillip J Dexheimer,Rebekah Karns,Tzipi Braun,Mi-Ok Kim,Thomas D Walters,Robert N Baldassano,Joshua D Noe,Joel Rosh,James Markowitz,Wallace V Crandall,David R Mack,Anne M Griffiths,Melvin B Heyman,Susan S Baker,Richard Kellermayer,Dedrick Moulton,Ashish S Patel,Ajay S Gulati,Steven J Steiner,Neal LeLeiko,Anthony Otley,Maria Oliva-Hemker,David Ziring,Barbara S Kirschner,David J Keljo,Stephen L Guthery,Stanley A Cohen,Scott Snapper,Jonathan Evans,Marla Dubinsky,Bruce Aronow,Jeffrey S Hyams,Subra Kugathasan,Curtis Huttenhower,Ramnik J Xavier,Lee A Denson

Digestive and liver disease : official journal of 42:848-53 PubMed20452301

2010

Altered expression of innate immunity genes in different intestinal sites of children with ulcerative colitis.

Applications

WB

Species

Human

Laura Stronati,Anna Negroni,Maria Pierdomenico,Chiara D'Ottavio,Donatella Tirindelli,Giovanni Di Nardo,Salvatore Oliva,Franca Viola,Salvatore Cucchiara

PloS one 4:e7984 PubMed19956723

2009

Mucosal gene expression of antimicrobial peptides in inflammatory bowel disease before and after first infliximab treatment.

Applications

IHC-P

Species

Human

Ingrid Arijs,Gert De Hertogh,Katleen Lemaire,Roel Quintens,Leentje Van Lommel,Kristel Van Steen,Peter Leemans,Isabelle Cleynen,Gert Van Assche,Séverine Vermeire,Karel Geboes,Frans Schuit,Paul Rutgeerts

FEBS letters 580:5344-50 PubMed16989824

2006

Paneth cell antimicrobial peptides: topographical distribution and quantification in human gastrointestinal tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Wehkamp,Hiutung Chu,Bo Shen,Ryan W Feathers,Robert J Kays,Sarah K Lee,Charles L Bevins

Proceedings of the National Academy of Sciences of 102:18129-34 PubMed16330776

2005

Reduced Paneth cell alpha-defensins in ileal Crohn's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Wehkamp,Nita H Salzman,Edith Porter,Sabine Nuding,Michael Weichenthal,Robert E Petras,Bo Shen,Elke Schaeffeler,Matthias Schwab,Rose Linzmeier,Ryan W Feathers,Hiutung Chu,Heriberto Lima,Klaus Fellermann,Tomas Ganz,Eduard F Stange,Charles L Bevins
View all publications
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Product promise

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