AB7654

Anti-alpha Internexin antibody [2E3]

Name: Anti-alpha Internexin antibody [2E3] (ab7654) | Abcam
Brand: Abcam Limited
SKU: AB7654
Price: 565 USD
Availability: InStock
Rating: 2 (3 reviews)

(3 Reviews)

(3 Publications)

Mouse Monoclonal alpha Internexin antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Rat, Mouse, Cow samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Ina.

View Alternative Names

NEF5, INA, Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, Neurofilament 5, NF-66, Neurofilament-66

3 Images

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Western blot - Anti-alpha Internexin antibody [2E3] (AB7654)

All lanes:

Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)

Lanes 1 - 2:

Crude homogenate of rat facial nucleus at 3 days after experimental injury

Lanes 3 - 4:

Crude homogenate of rat facial nucleus at 5 days after experimental injury

Lanes 5 - 6:

Crude homogenate of rat facial nucleus at 7 days after experimental injury

Lanes 7 - 8:

Crude homogenate of rat facial nucleus at 14 days after experimental injury

Lanes 9 - 10:

Crude homogenate of rat facial nucleus at 28 days after experimental injury

Predicted band size: 55 kDa

Observed band size: 66 kDa

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Supplier Data

Western blot - Anti-alpha Internexin antibody [2E3] (AB7654)

All lanes:

Western blot - Anti-alpha Internexin antibody [2E3] (ab7654) at 1/10000 dilution

Lane 1:

Rat brain lysate

Lane 2:

Rat spinal cord lysate

Lane 3:

Mouse brain lysate

Lane 4:

Mouse spinal cord lysate

Lane 5:

Cow spinal cord lysate

Predicted band size: 55 kDa

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Flow Cytometry - Anti-alpha Internexin antibody [2E3] (AB7654)

Flow Cyt

Unknown

Flow Cytometry - Anti-alpha Internexin antibody [2E3] (AB7654)

Overlay histogram showing SH-SY5Y cells stained with ab7654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7654, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2E3

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Cow, Human

Applications

ICC/IF, Flow Cyt, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Rat Ina.

P23565

Epitope

C-terminal non-helical extension of the protein.

Specificity

specifically recognizes a-internexin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Mammals": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Internexin also known as alpha-Int or INA is a neuronal intermediate filament protein with a molecular mass of approximately 66 kDa. It plays a significant role in the formation of the neuronal cytoskeleton providing structural support to axons. Alpha Internexin is expressed mainly in the central nervous system and is found in neurons throughout the brain and spinal cord. Despite sharing functions with other intermediate filament proteins like NF-L alpha Internexin is unique in its distribution and timing of expression during neural development.

Biological function summary

Alpha Internexin contributes to the composition of the neurofilament complex which includes proteins like NF-M NF-L and NF-H. This complex stabilizes neuronal architecture and ensures the integrity of the axonal structure. Presence of alpha Internexin is important for maintaining the dynamic processes of axonal transport and neuron stability. It acts as a scaffolding that supports cellular transport mechanisms important for neuron function and survival especially in developing neurons where it leads the polymerization of other neurofilaments.

Pathways

Alpha Internexin interacts within the neurofilament network a major scaffolding component of neurons. It associates with microtubule-associated proteins influencing axonal growth and stabilization pathways. Moreover it plays a part in the MAPK (mitogen-activated protein kinases) signaling pathways which regulate diverse cellular activities such as proliferation and differentiation. Its interactions with related proteins such as plectin and dynein highlight its role in crosslinking cytoskeletal components and facilitating intracellular transport.

Alpha Internexin has been implicated in neurodegenerative conditions such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. Misregulation or mutation in alpha Internexin can contribute to the pathological hallmarks of these diseases including protein aggregation and neuronal death. In ALS the presence of alpha Internexin inclusions correlates with disease pathology alongside other neurofilament proteins like NF-L. In Alzheimer's disease abnormal phosphorylation and aggregation of alpha Internexin are associated with neurofibrillary tangles which are indicative of neuronal injury and loss.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges. May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks (By similarity).

See full target information INA

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 12:777-89 PubMed16132113

2005

The expression of alpha-internexin and peripherin in the developing mouse pineal gland.

Applications

Unspecified application

Species

Unspecified reactive species

Tsui-Ling Ko,Chung-Liang Chien,Kuo-Shyan Lu

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54:39-46 PubMed16087706

2005

Expression of intermediate filament proteins and neuronal markers in the human fetal gut.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrich Rauch,Markus Klotz,Silke Maas-Omlor,Elvira Wink,Andrea Hänsgen,Cornelia Hagl,Stefan Holland-Cunz,Karl-Herbert Schäfer

Neurology 64:1658-9 PubMed15883343

2005

Screening for neurofilament inclusion disease using alpha-internexin immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Hirotake Uchikado,Gerry Shaw,Deng-Shun Wang,Dennis W Dickson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Internexin antibody [2E3]

Western blot - Anti-alpha Internexin antibody [2E3] (AB7654)

Western blot - Anti-alpha Internexin antibody [2E3] (AB7654)

Flow Cytometry - Anti-alpha Internexin antibody [2E3] (AB7654)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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