Rabbit Polyclonal alpha Internexin antibody. Suitable for WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Mouse, Rat, Cow, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Ina.
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- Neuron 106:607-623.e52020Oxygen Tension and the VHL-Hif1α Pathway Determine Onset of Neuronal Polarization and Cerebellar Germinal Zone Exit.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJan A Kullmann et. al.PubMed 32183943
- The Journal of comparative neurology 523:2111-262015Postnatal accumulation of intermediate filaments in the cat and human primary visual cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeoho Song,Donald E Mitchell,Nathan A Crowder,Kevin R DuffyPubMed 25823892
- Acta neuropathologica 130:145-572015A truncating SOD1 mutation, p.Gly141X, is associated with clinical and pathologic heterogeneity, including frontotemporal lobar degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasataka Nakamura,Kevin F Bieniek,Wen-Lang Lin,Neill R Graff-Radford,Melissa E Murray,Monica Castanedes-Casey,Pamela Desaro,Matthew C Baker,Nicola J Rutherford,Janice Robertson,Rosa Rademakers,Dennis W Dickson,Kevin B BoylanPubMed 25917047
- Cell 152:1119-332013Encoding and transducing the synaptic or extrasynaptic origin of NMDA receptor signals to the nucleus.Applications:ICC/IF, WBReactive species:RabbitAnna Karpova,Marina Mikhaylova,Sujoy Bera,Julia Bär,Pasham Parameshwar Reddy,Thomas Behnisch,Vladan Rankovic,Christina Spilker,Philipp Bethge,Jale Sahin,Rahul Kaushik,Werner Zuschratter,Thilo Kähne,Michael Naumann,Eckart D Gundelfinger,Michael R KreutzPubMed 23452857
- Nature medicine 18:1052-92012Profiling of residual breast cancers after neoadjuvant chemotherapy identifies DUSP4 deficiency as a mechanism of drug resistance.Applications:WBReactive species:Unspecified reactive speciesJustin M Balko,Rebecca S Cook,David B Vaught,María G Kuba,Todd W Miller,Neil E Bhola,Melinda E Sanders,Nara M Granja-Ingram,J Joshua Smith,Ingrid M Meszoely,Janine Salter,Mitch Dowsett,Katherine Stemke-Hale,Ana M González-Angulo,Gordon B Mills,Joseph A Pinto,Henry L Gómez,Carlos L ArteagaPubMed 22683778
- Brain : a journal of neurology 134:2478-922011The clinical diagnosis of early-onset dementias: diagnostic accuracy and clinicopathological relationships.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulie S Snowden,Jennifer C Thompson,Cheryl L Stopford,Anna M T Richardson,Alex Gerhard,David Neary,David M A MannPubMed 21840888
- Hippocampus 21:108-192009Proteomics reveal rat hippocampal lateral asymmetry.Applications:WBReactive species:RatA Samara,K Vougas,A Papadopoulou,E Anastasiadou,N Baloyanni,E Paronis,G P Chrousos,G Th TsangarisPubMed 20020437
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.05% Sodium azide, 0.01% Thimerosal (merthiolate)
Constituents: 1.5% BSA, 1.45% Sodium chloride, 0.164% Sodium phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Rat Ina. Database link P23565
Reactivity data
Select an application| WB | IHC-FoFr | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Expected | Tested | Expected |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Tested | Tested | Expected | Tested |
| Cow | Tested | Expected | Expected | Expected |
| Mammals | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000.00000 - 1/20000.00000 | Notes - |
Species Rat | Dilution info 1/10000.00000 - 1/20000.00000 | Notes - |
Species Cow | Dilution info 1/10000.00000 - 1/20000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IHC-FoFr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
Associated Products
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Target data
Function
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges (By similarity). May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks (By similarity).
Alternative names
Inexa, Ina, Alpha-internexin, Alpha-Inx
Recommended products
Rabbit Polyclonal alpha Internexin antibody. Suitable for WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Mouse, Rat, Cow, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Ina.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.05% Sodium azide, 0.01% Thimerosal (merthiolate)
Constituents: 1.5% BSA, 1.45% Sodium chloride, 0.164% Sodium phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Rat Ina. Database link P23565
- Purity
- Whole antiserum
- Specificity
Specifically recognizes a-internexin.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha Internexin also known as alpha-Int or INA is a neuronal intermediate filament protein with a molecular mass of approximately 66 kDa. It plays a significant role in the formation of the neuronal cytoskeleton providing structural support to axons. Alpha Internexin is expressed mainly in the central nervous system and is found in neurons throughout the brain and spinal cord. Despite sharing functions with other intermediate filament proteins like NF-L alpha Internexin is unique in its distribution and timing of expression during neural development.
- Biological function summary
Alpha Internexin contributes to the composition of the neurofilament complex which includes proteins like NF-M NF-L and NF-H. This complex stabilizes neuronal architecture and ensures the integrity of the axonal structure. Presence of alpha Internexin is important for maintaining the dynamic processes of axonal transport and neuron stability. It acts as a scaffolding that supports cellular transport mechanisms important for neuron function and survival especially in developing neurons where it leads the polymerization of other neurofilaments.
- Pathways
Alpha Internexin interacts within the neurofilament network a major scaffolding component of neurons. It associates with microtubule-associated proteins influencing axonal growth and stabilization pathways. Moreover it plays a part in the MAPK (mitogen-activated protein kinases) signaling pathways which regulate diverse cellular activities such as proliferation and differentiation. Its interactions with related proteins such as plectin and dynein highlight its role in crosslinking cytoskeletal components and facilitating intracellular transport.
- Associated diseases and disorders
Alpha Internexin has been implicated in neurodegenerative conditions such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. Misregulation or mutation in alpha Internexin can contribute to the pathological hallmarks of these diseases including protein aggregation and neuronal death. In ALS the presence of alpha Internexin inclusions correlates with disease pathology alongside other neurofilament proteins like NF-L. In Alzheimer's disease abnormal phosphorylation and aggregation of alpha Internexin are associated with neurofibrillary tangles which are indicative of neuronal injury and loss.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-alpha Internexin antibody (ab7259)
All lanes: Western blot - Anti-alpha Internexin antibody (ab7259) at 1/10000 dilution
Lane 1: Protein standard
Lane 2: Mouse spinal cord lysate
Lane 3: Rat spinal cord lysate
Lane 4: Cow spinal cord lysate
Predicted band size: 55 kDa
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-alpha Internexin antibody (ab7259)
Immunofluorescence analysis of rat cerebellum section stained for alpha Internexin using ab7259 at a 1/2000 dilution (green) co-strained with chicken polyclonal antibody to GFAP at a 1/5000 dilution (red). DAPI was used to stain nuclear DNA (blue).
Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody (ab7259)
ab7259 shows red, neuronal progenitor cells, plectin (not one of our antibodies) shows fibroblast marker. Photo courtesy of: Dr. Gerry Shaw University of Florida
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody (ab7259)
ab7259 staining Human normal temporal cortex. Staining is localised to intracellualr compartment.
Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody (ab7259)
ICC/IF image of ab7259 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7259, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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