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AB40758

Anti-alpha Internexin antibody [EP676Y]

4

(7 Reviews)

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(9 Publications)

Rabbit Recombinant Monoclonal alpha Internexin antibody. Suitable for WB, IHC-P, ICC/IF, IP, Flow Cyt, IHC-FoFr and reacts with Human, Rat, Mouse samples. Cited in 9 publications.

View Alternative Names

NEF5, INA, Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, Neurofilament 5, NF-66, Neurofilament-66

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EP676Y] (AB40758)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EP676Y] (AB40758)

Positive staining of alpha Internexin on paraffin-embedded human brain tissue (A) using ab40758 diluited 1 : 250. Negative staining of paraffin-embedded human colon (B), Kidney (C) and uterus (D) tissues using ab40758 under the same conditions.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody [EP676Y] (AB40758)
  • ICC/IF

AbReview43441****

Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody [EP676Y] (AB40758)

ab40758 staining alpha Internexin in rat glial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 10 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody [EP676Y] (AB40758)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody [EP676Y] (AB40758)

Immunofluorescent staining of alpha Internexin on PC 12 cells using ab40758 diluted 1 : 250.

Western blot - Anti-alpha Internexin antibody [EP676Y] (AB40758)
  • WB

Lab

Western blot - Anti-alpha Internexin antibody [EP676Y] (AB40758)

Lanes 1-4 : Merged signal (red and green). Green - ab40758 observed at 66 kDa. Red - loading control ab8245 observed at 36 kDa.

ab40758 Anti-alpha Internexin antibody [EP676Y] was shown to specifically react with alpha Internexin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265264 (knockout cell lysate ab257482) was used. Wild-type and alpha Internexin knockout samples were subjected to SDS-PAGE. ab40758 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-alpha Internexin antibody [EP676Y] (ab40758) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

INA knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human INA (alpha Internexin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ina-alpha-internexin-knockout-hela-cell-line-ab265264'>ab265264</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 52 kDa,55 kDa

Observed band size: 60 kDa,66 kDa

false

Western blot - Anti-alpha Internexin antibody [EP676Y] (AB40758)
  • WB

Unknown

Western blot - Anti-alpha Internexin antibody [EP676Y] (AB40758)

All lanes:

Western blot - Anti-alpha Internexin antibody [EP676Y] (ab40758) at 1/10000 dilution

All lanes:

SHSY5Y cell lysate at 10 µg

Predicted band size: 55 kDa

Observed band size: 60 kDa

false

  • Carrier free

    Anti-alpha Internexin antibody [EP676Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP676Y

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

IHC-FoFr, ICC/IF, IP, WB, IHC-P, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Internexin also known as alpha-Int or INA is a neuronal intermediate filament protein with a molecular mass of approximately 66 kDa. It plays a significant role in the formation of the neuronal cytoskeleton providing structural support to axons. Alpha Internexin is expressed mainly in the central nervous system and is found in neurons throughout the brain and spinal cord. Despite sharing functions with other intermediate filament proteins like NF-L alpha Internexin is unique in its distribution and timing of expression during neural development.
Biological function summary

Alpha Internexin contributes to the composition of the neurofilament complex which includes proteins like NF-M NF-L and NF-H. This complex stabilizes neuronal architecture and ensures the integrity of the axonal structure. Presence of alpha Internexin is important for maintaining the dynamic processes of axonal transport and neuron stability. It acts as a scaffolding that supports cellular transport mechanisms important for neuron function and survival especially in developing neurons where it leads the polymerization of other neurofilaments.

Pathways

Alpha Internexin interacts within the neurofilament network a major scaffolding component of neurons. It associates with microtubule-associated proteins influencing axonal growth and stabilization pathways. Moreover it plays a part in the MAPK (mitogen-activated protein kinases) signaling pathways which regulate diverse cellular activities such as proliferation and differentiation. Its interactions with related proteins such as plectin and dynein highlight its role in crosslinking cytoskeletal components and facilitating intracellular transport.

Alpha Internexin has been implicated in neurodegenerative conditions such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. Misregulation or mutation in alpha Internexin can contribute to the pathological hallmarks of these diseases including protein aggregation and neuronal death. In ALS the presence of alpha Internexin inclusions correlates with disease pathology alongside other neurofilament proteins like NF-L. In Alzheimer's disease abnormal phosphorylation and aggregation of alpha Internexin are associated with neurofibrillary tangles which are indicative of neuronal injury and loss.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges. May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks (By similarity).
See full target information INA
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 21:e13541 PubMed34953016

2021

Chemically induced senescence in human stem cell-derived neurons promotes phenotypic presentation of neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Fathi,Sakthikumar Mathivanan,Linghai Kong,Andrew J Petersen,Cole R K Harder,Jasper Block,Julia Marie Miller,Anita Bhattacharyya,Daifeng Wang,Su-Chun Zhang

Scientific reports 11:14001 PubMed34234233

2021

The UL16 protein of HSV-1 promotes the metabolism of cell mitochondria by binding to ANT2 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyu Li,Shuting Liu,Zhenning Dai,Qian Zhang,Yichao Xu,Youyu Chen,Zhenyou Jiang,Wenhua Huang,Hanxiao Sun

Nature communications 12:3058 PubMed34031387

2021

Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmin Morandell,Lena A Schwarz,Bernadette Basilico,Saren Tasciyan,Georgi Dimchev,Armel Nicolas,Christoph Sommer,Caroline Kreuzinger,Christoph P Dotter,Lisa S Knaus,Zoe Dobler,Emanuele Cacci,Florian K M Schur,Johann G Danzl,Gaia Novarino

Journal of neuroscience research 96:1798-1815 PubMed30054946

2018

Selenium positively affects the proteome of 3 × Tg-AD mice cortex by altering the expression of various key proteins: unveiling the mechanistic role of selenium in AD prevention.

Applications

Unspecified application

Species

Unspecified reactive species

Javed Iqbal,Kaoyuan Zhang,Na Jin,Yuxi Zhao,Qiong Liu,Jiazuan Ni,Liming Shen

Aging cell 15:924-39 PubMed27448508

2016

Proteins that mediate protein aggregation and cytotoxicity distinguish Alzheimer's hippocampus from normal controls.

Applications

IHC-P

Species

Human

Srinivas Ayyadevara,Meenakshisundaram Balasubramaniam,Paul A Parcon,Steven W Barger,W Sue T Griffin,Ramani Alla,Alan J Tackett,Samuel G Mackintosh,Emanuel Petricoin,Weidong Zhou,Robert J Shmookler Reis

Clinical autonomic research : official journal of 26:307-13 PubMed27369358

2016

Autonomic nervous system involvement in the giant axonal neuropathy (GAN) KO mouse: implications for human disease.

Applications

Unspecified application

Species

Unspecified reactive species

Diane Armao,Rachel M Bailey,Thomas W Bouldin,Yongbaek Kim,Steven J Gray

Oncotarget 7:27855-73 PubMed27034165

2016

Spatial memory impairment by TRPC1 depletion is ameliorated by environmental enrichment.

Applications

WB

Species

Unspecified reactive species

Renzhong Xing,Yanling Zhang,Hua Xu,Xiaobin Luo,Raymond Chuen-Chung Chang,Jianjun Liu,Xifei Yang

The Journal of biological chemistry 291:5221-33 PubMed26740621

2016

Ubiquilin-mediated Small Molecule Inhibition of Mammalian Target of Rapamycin Complex 1 (mTORC1) Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Rory T Coffey,Yuntao Shi,Marcus J C Long,Michael T Marr,Lizbeth Hedstrom

Neuroscience letters 518:49-54 PubMed22561033

2012

Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Elan D Louis,Karen Ma,Rachel Babij,Etty Cortés,Ronald K Liem,Jean-Paul G Vonsattel,Phyllis L Faust
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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