AB247623

Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal alpha Internexin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 2 publications.

View Alternative Names

NEF5, INA, Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, Neurofilament 5, NF-66, Neurofilament-66

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

This data was developed using ab108302, the same antibody clone in a different buffer formulation.

ab108302 showing negative staining in Normal human colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

This data was developed using ab108302, the same antibody clone in a different buffer formulation.

ab108302, at 1/250, staining alpha Internexin in human brain tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

This data was developed using ab108302, the same antibody clone in a different buffer formulation.

ab108302 showing negative staining in Normal human tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

This data was developed using ab108302, the same antibody clone in a different buffer formulation.

ab108302 showing negative staining in Normal human liver tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

This data was developed using ab108302, the same antibody clone in a different buffer formulation.

ab108302 showing negative staining in Normal human breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

This data was developed using ab108302, the same antibody clone in a different buffer formulation.

Secondary antibody - goat anti-rabbit HRP (ab6721).

All lanes:

Western blot - Anti-alpha Internexin antibody [EPR1529] (<a href='/en-us/products/primary-antibodies/alpha-internexin-antibody-epr1529-ab108302'>ab108302</a>) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

Y79 cell lysate at 10 µg

Lane 3:

Mouse brain lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1529

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab247623 is the carrier-free version of ab108302.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Internexin also known as alpha-Int or INA is a neuronal intermediate filament protein with a molecular mass of approximately 66 kDa. It plays a significant role in the formation of the neuronal cytoskeleton providing structural support to axons. Alpha Internexin is expressed mainly in the central nervous system and is found in neurons throughout the brain and spinal cord. Despite sharing functions with other intermediate filament proteins like NF-L alpha Internexin is unique in its distribution and timing of expression during neural development.

Biological function summary

Alpha Internexin contributes to the composition of the neurofilament complex which includes proteins like NF-M NF-L and NF-H. This complex stabilizes neuronal architecture and ensures the integrity of the axonal structure. Presence of alpha Internexin is important for maintaining the dynamic processes of axonal transport and neuron stability. It acts as a scaffolding that supports cellular transport mechanisms important for neuron function and survival especially in developing neurons where it leads the polymerization of other neurofilaments.

Pathways

Alpha Internexin interacts within the neurofilament network a major scaffolding component of neurons. It associates with microtubule-associated proteins influencing axonal growth and stabilization pathways. Moreover it plays a part in the MAPK (mitogen-activated protein kinases) signaling pathways which regulate diverse cellular activities such as proliferation and differentiation. Its interactions with related proteins such as plectin and dynein highlight its role in crosslinking cytoskeletal components and facilitating intracellular transport.

Alpha Internexin has been implicated in neurodegenerative conditions such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. Misregulation or mutation in alpha Internexin can contribute to the pathological hallmarks of these diseases including protein aggregation and neuronal death. In ALS the presence of alpha Internexin inclusions correlates with disease pathology alongside other neurofilament proteins like NF-L. In Alzheimer's disease abnormal phosphorylation and aggregation of alpha Internexin are associated with neurofibrillary tangles which are indicative of neuronal injury and loss.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges. May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks (By similarity).

See full target information INA

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

TH open : companion journal to thrombosis and haemostasis 7:e217-e225 PubMed37501780

2023

Levels of Fibrinogen Variants Are Altered in Severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Judith J de Vries,Chantal Visser,Maureen van Ommen,Casper Rokx,Els van Nood,Eric C M van Gorp,Marco Goeijenbier,Johannes P C van den Akker,Henrik Endeman,Dingeman C Rijken,Marieke J H A Kruip,Miranda Weggeman,Jaap Koopman,Moniek P M de Maat

Frontiers in cellular neuroscience 12:161 PubMed29967576

2018

Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human Stroke Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca Mages,Susanne Aleithe,Stephan Altmann,Alexandra Blietz,Björn Nitzsche,Henryk Barthel,Anja K E Horn,Constance Hobusch,Wolfgang Härtig,Martin Krueger,Dominik Michalski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

Western blot - Anti-alpha Internexin antibody [EPR1529] - BSA and Azide free (AB247623)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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