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Mouse Monoclonal alpha Lactalbumin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-alpha Lactalbumin antibody [F20.16] (AB20212), expandable thumbnail

Publications

Key facts

Isotype
IgM
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Associated Products

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Target data

Function

Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.

Additional Targets

alpha Lactalbumin

Alternative names

LYZL7, LALBA, Alpha-lactalbumin, Lactose synthase B protein, Lysozyme-like protein 7

Recommended products

Mouse Monoclonal alpha Lactalbumin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgM
Form
Liquid
Clonality
Monoclonal
Clone number
F20.16
Purity
IgM fraction
Light chain type
unknown
Concentration
Loading...
Purification notes

This antibody was purified using affinity chromatography. The sample was eluted using a commercially bought Neutral Elution Buffer and exchanged into PBS using a G25 column.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes


This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha-lactalbumin commonly known as lactalbumin or alpha-lactoglobulin has a mass of approximately 14.2 kDa. This protein is mainly expressed in the mammary glands of mammals where it serves an important function in lactose synthesis. It modifies the action of galactosyltransferase an enzyme to enable the transfer of sugar units resulting in the production of lactose. The biochemical role of this lactalbumin protein including its unique structure is important in adapting the lactation system to meet the nutritional needs of offspring.

Biological function summary

Alpha-lactalbumin's role is closely linked to the synthesis and secretion of milk. It functions as part of the lactose synthase complex which is essential for converting glucose to lactose. This conversion is important because lactose dictates milk osmotic pressure affecting milk volume and overall composition. In addition to its primary role in milk production this protein structure supports various cellular processes due to its ability to bind calcium and other ions.

Pathways

Alpha-lactalbumin participates mainly in the lactose synthesis pathway. In this metabolic map it interacts extensively with other proteins involved in milk production particularly beta-14-galactosyltransferase. This interaction enhances the efficiency of lactose production. Its presence in these pathways epitomizes the close relationship and cellular coordination needed for effective lactose synthesis during lactation.

Associated diseases and disorders

Researchers have identified connections between alpha-lactalbumin and breast cancer as well as lactose intolerance. Changes in the expression levels of this protein can impact the development and regulation of tumors in breast tissue linked to interactions with proteins such as casein. Furthermore variants of alpha-lactalbumin can affect individuals with lactose intolerance where the protein's role in regulating lactose production must be managed to alleviate symptoms.

Product promise

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Lactalbumin antibody [F20.16] (ab20212), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-alpha Lactalbumin antibody [F20.16] (ab20212)

    ICC/IF image of ab20212 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20212, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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