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Rabbit Recombinant Monoclonal Casein antibody. Suitable for WB, IHC-P, IP and reacts with Transfected cell lysate - Cow, Cow, Transfected cell line - Cow samples.

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Images

Immunoprecipitation - Anti-Alpha-S1-casein antibody [EPR29093-719] (AB323188), expandable thumbnail
  • Western blot - Anti-Alpha-S1-casein antibody [EPR29093-719] (AB323188), expandable thumbnail
  • Western blot - Anti-Alpha-S1-casein antibody [EPR29093-719] (AB323188), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-S1-casein antibody [EPR29093-719] (AB323188), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIP
Cow
Tested
Expected
Expected
Transfected cell line - Cow
Not recommended
Tested
Not recommended
Transfected cell lysate - Cow
Tested
Not recommended
Tested

Tested
Tested

Species
Transfected cell lysate - Cow
Dilution info
1/1000
Notes

-

Species
Cow
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Cow
Dilution info
1/2000
Notes

-

Expected
Expected

Species
Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Cow
Dilution info
1/30
Notes

-

Expected
Expected

Species
Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Cow
Dilution info
-
Notes

-

Target data

Function

Important role in the capacity of milk to transport calcium phosphate. Antioxidant peptide has 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity.

Alternative names

Alpha-S1-casein

Rabbit Recombinant Monoclonal Casein antibody. Suitable for WB, IHC-P, IP and reacts with Transfected cell lysate - Cow, Cow, Transfected cell line - Cow samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29093-719
Purification technique
Affinity purification Protein A
Specificity

Please avoid using buffer with milk and BSA for blocking and dilution.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-Alpha-S1-casein antibody [EPR29093-719] (ab323188), expandable thumbnail

    Immunoprecipitation - Anti-Alpha-S1-casein antibody [EPR29093-719] (ab323188)

    Alpha-S1-casein was immunoprecipitated from 0.35 mg 293T cells transfected with a Bovine Alpha-S1-casein expression vector containing a myc-His-tag®, whole cell lysate with ab323188 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323188 at 1/1000 dilution.

    Blocking and dilution buffer and concentration: Blocking Buffer diluted with an equal volume of TBS

    Anti-Alpha-S1-casein antibody (ab323188) staining at 1/1000 dilution, shown in green(down); Mouse anti-GAPDH antibody 6C5 loading control staining at 1/20000 dilution, shown in magenta;IgG heavy chain show in green(up).

    Avoid using milk-based buffers for blocking and dilution, as they might cause interference.

    All lanes: Immunoprecipitation - Anti-Alpha-S1-casein antibody [EPR29093-719] (ab323188) at 1/1000 dilution

    Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

    Lane 2: ab323188 at 1/30 IP in HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab323188 in 293T cellstransfected with a Bovine Alpha-S1-casein expression vectorcontaining a myc-His-tag®, whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 34 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Alpha-S1-casein antibody [EPR29093-719] (ab323188), expandable thumbnail

    Western blot - Anti-Alpha-S1-casein antibody [EPR29093-719] (ab323188)

    Alpha-S1-casein Western blot staining using rabbit Anti-Alpha-S1-casein antibody

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS.

    The samples were run on a Bis-Tris gel under reducing conditions.

    Western blot: Anti-Alpha-S1-casein antibody (ab323188) staining at 1/1000 dilution, shown in green(up); Mouse anti-GAPDH antibody 6C5 loading control staining at 1/20000 dilution, shown in magenta; Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) at 1/5000 dilution, shown in green(down).

    Milk Powder Trim was diluted using 1%TBS buffer.

    Avoid using milk-based buffers for blocking and dilution, as they might cause interference.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Alpha-S1-casein antibody [EPR29093-719] (ab323188) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a Bovine Alpha-S1-casein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    Lanes 1 - 2: Goat Anti-Rabbit IgG H&L (800CW) at 1/100000 dilution

    Lanes 1 - 2: Goat Anti-Mouse IgG H&L (680RD) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 34 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Alpha-S1-casein antibody [EPR29093-719] (ab323188), expandable thumbnail

    Western blot - Anti-Alpha-S1-casein antibody [EPR29093-719] (ab323188)

    Alpha-S1-casein Western blot staining using rabbit Anti-Alpha-S1-casein antibody

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS.

    The samples were run on a Bis-Tris gel under reducing conditions.

    Milk Powder Trim was diluted using 1%TBS buffer.

    There is an empty lane between lane1 and lane2.

    Western blot: Anti-Alpha-S1-casein antibody (ab323188) staining at 1/1000 dilution, shown in green.

    Avoid using milk-based buffers for blocking and dilution, as they might cause interference.

    All lanes: Western blot - Anti-Alpha-S1-casein antibody [EPR29093-719] (ab323188) at 1/1000 dilution

    Lane 1: Milk whole lysate at 20 µg

    Lane 2: Milk whole lysate at 10 µg

    Secondary

    Lanes 1 - 2: Goat Anti-Rabbit IgG H&L (800CW) at 1/100000 dilution

    Lanes 1 - 2: Goat Anti-Mouse IgG H&L (680RD) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 34 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-S1-casein antibody [EPR29093-719] (ab323188), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-S1-casein antibody [EPR29093-719] (ab323188)

    Alpha-S1-casein Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Alpha-S1-casein antibody

    Immunohistochemical analysis of paraffin-embedded Panel A 293T (human embryonic kidney epithelial cell) cells transfected with a Bovine Alpha-S1-casein expression vector containing a his tag. Panel B 293T cells transfected with empty vector containing a his tag. tissue labeling Alpha-S1-casein with ab323188 at 1/2000 (0.25ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) 293T transfected with a Bovine Alpha-S1-casein expression vector, no staining on (B) 293T transfected with empty vector.

    The section was incubated with ab323188 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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